Antioxidant Effect of Dayak Onion Extract (Eleutherine Americana Merr.) on Serum MDA Levels in Mice (Mus Musculus) Exposed by Lead Acetate

Abstract

Phenols and flavonoids are the antioxidants contained in Dayak bulb extract that can eliminate the exposure of free radicals. Lead is one of the heavy metals toxic used by industries. It can increase ROS in the body due to pollution that occurs in the environment. The lead that enters the body is excreted throughout the body tissues. The lead accumulated in the blood can inhibit δ-ALAD enzyme function in the synthesis of heme to form a complex bond between the δ-ALAD enzyme and oxyhemoglobin that results in reactive bonds such as hydrogen peroxide, superoxide radicals and also produces ROS in the body. The formation of ROS can damage lipid peroxide from cell membranes by spreading a chain reaction. MDA is an indicator of free radicals emerging in the body. This study aims to find out whether the extract of Dayak onion can prevent the increase of serum MDA levels in mice exposed by lead acetate. Dayak fresh bulbs extracted with 96% ethanol solvent were administrated orally at 65 mg/kgBW, 130 mg/kgBW, and 260 mg/kgBW for 21 days, along with the administration of lead acetate for 14 days on the 8 th -21 st day. The serum MDA levels were examined using the TBA test method. The expected results showed that the serum level tended to experience a decrease in the treatment group rather than in the control group (p<0.05). It can be concluded that the extract of Dayak onion can prevent the increased serum MDA levels in mice exposed by lead acetate.