Antioxidant effect of crocin on bovine sperm quality and in vitro fertilization

Sapanidou, V.; Taitzoglou, I.; Tsakmakidis, Ιoannis; Kourtzelis, Ioannis; Fletouris, Dimitrios; Theodoridis, Alexandros; Zervos, Ioannis; Tsantarliotou, Maria

doi:10.1016/j.theriogenology.2015.07.005

Cited by 69 publications

(79 citation statements)

References 49 publications

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“…As described in our previous study [23], ROS was major generated in sperm mitochondria, and in the literature, elevated ROS level has been reported during cooling, freezing and thawing in human [3], bovine [43] and ram [44] spermatozoa. ROS was produced during post-thaw incubation in bovine [45], ram [46] and red deer [47] spermatozoa. In the present study, we observed that rabbit sperm accumulated ROS and H 2 O 2 during cooling, equilibration, freeze-thaw, and post-thaw incubation, which was in agreement with the aforementioned observation in the other animal spermatozoa.…”

Section: Discussionmentioning

confidence: 99%

Cysteine protects rabbit spermatozoa against reactive oxygen species-induced damages

et al. 2017

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The process of cryopreservation results in over-production of reactive oxygen species, which is extremely detrimental to spermatozoa. The aim of this study was to investigate whether addition of cysteine to freezing extender would facilitate the cryosurvival of rabbit spermatozoa, and if so, how cysteine protects spermatozoa from cryodamages. Freshly ejaculated semen was diluted with Tris-citrate-glucose extender supplemented with different concentrations of cysteine. The motility, intact acrosomes, membrane integrity, mitochondrial potentials, 8-hydroxyguanosine level and sperm-zona pellucida binding capacity were examined. Furthermore, glutathione peroxidase (GPx) activity, glutathione content (GSH), and level of reactive oxygen species (ROS) and hydrogen peroxide of spermatozoa were analyzed. The values of motility, intact acrosomes, membrane integrity, mitochondrial potentials and sperm-zona pellucida binding capacity of the frozen-thawed spermatozoa in the treatment of cysteine were significantly higher than those of the control. Addition of cysteine to extenders improved the GPx activity and GSH content of spermatozoa, while lowered the ROS, DNA oxidative alterations and lipid peroxidation level, which makes spermatozoa avoid ROS to attack DNA, the plasma membrane and mitochondria. In conclusion, cysteine protects spermatozoa against ROS-induced damages during cryopreservation and post-thaw incubation. Addition of cysteine is recommended to facilitate the improvement of semen preservation for the rabbit breeding industry.

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Section: Discussionmentioning

confidence: 99%

Cysteine protects rabbit spermatozoa against reactive oxygen species-induced damages

et al. 2017

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“…More recent studies using flow cytometry associated dyes such as YoPro-1 (SAPANIDOU et al, 2015), which assesses PM stability with the Merocyanine 540, which evaluates PM permeability. The combination of Merocyanine 540 with YoPro-1 and Hoechst 33342 was also used to assesses the stability, permeability and integrity of PM as described by Hallap et al (2006).…”

Section: Association Of Fluorescent Probesmentioning

confidence: 99%

“…Therefore, many researchers investigated the development of laboratory tests that evaluate sperm cells with greater accuracy, higher objectivity and repeatability (CELEGHINI et al, 2007;CARVALHO et al, 2010;HOSSAIN et al, 2011;STANDERHOLEN et al, 2014;SAPANIDOU et al, 2015). No single evaluation technique is sufficiently sensitive to determine fertility, but combinations of various parameters more accurately estimate the fertilization potential of a sperm sample PEÑA, 2015).…”

Section: Introductionmentioning

confidence: 99%

Techniques for sperm evaluation using fluorescent probes

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2015

SCA

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A variety of laboratory tests were developed to obtain more reliable results of sperm evaluation and increase the accuracy of sperm fertility predictions. These tests detected damage of sperm specific compartments or organelles, which cannot be detected in routine sperm analysis. The use of fluorescent probes and detection using fluorescent microscopy or flow cytometry is an important tool but a more precise and accurate laboratory test is needed. Propidium iodide and 6-carboxyfluorescein diacetate are used for evaluations of plasmatic membrane integrity. Fluorescein isothiocyanate, associated with conjugated lecithin Psium sativum or Arachis hypogaea, are used for evaluations of acrosome integrity. Two probes, MitoTracker or Rhodamine123, are generally used to measure the absence or presence of mitochondrial potential. However, a better option is 5,5'; 6,6' -tetrachloro -1,1'; 3,3' -tetraetilbenzimidazolil-carbocyanine (JC-1) dye, which assesses not only the presence of mitochondrial potential and distinguished spermatozoa with poorly and highly functional mitochondria. Two techniques, TUNEL or COMETA, and the Acridine Orange Test (AOT) dye are used to evaluate chromatin integrity. A fluorescence technique based on chlortetracycline (CTC) or Merocyanine 540 is used to estimate whether sperm pass by or through the capacitation process. This review focuses on the fluorescent probes that are most widely used to evaluate plasma membrane integrity, capacitation, acrosome integrity, chromatin integrity and mitochondrial potential. Key words: Fluorescence microscopy, sperm, cryopreservation ResumoDiversos testes laboratoriais têm sido desenvolvidos com o intuito de obter resultados confiáveis nas avaliações espermáticas, aumentando a qualidade e a confiabilidade do sêmen utilizado em técnicas de reprodução assistida. Esses testes têm permitido detectar danos em compartimentos e organelas específicas da célula espermática, que não são detectados nas análises de rotina. Dentre esses testes, o uso de sondas fluorescentes e sua detecção em microscópio de epifluorescência ou citometria de fluxo se tornaram ferramenta importante quando uma avaliação mais acurada é necessária. Para a avaliação da integridade de membrana plasmática, pode ser utilizado o iodeto de propídio, associado ao diacetato de 6-carboxifluoresceína. As avaliações de integridade acrossomal podem ser feitas através do isotiocianato de fluoresceína, associado às lecitinas conjugadas, como a Psium sativum ou Arachis hypogaea. O potencial mitocondrial pode ser avaliado quanto à ausência ou presença através da sonda Mitotracker ou Rodamina 123. Outra opção ainda melhor pode ser observada utilizando o corante iodeto de 5,5'; 6,6' -tetracloro -1,1'; 3,3' -tetraetilbenzimidazolil-carbocianina (JC-1), este corante avalia a presença do potencial mitocondrial e avalia quanto à classificação do grau. Para avaliar a integridade

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“…Similar to our study, Sapanidou et al . () detected a significant improvement in bovine sperm motility and viability with crocin added post‐thawing at concentrations in the mmol l −1 range after 2 h of incubation.…”

Section: Discussionmentioning

confidence: 98%

“…Crocin applied to semen after thawing was an effective ROS scavenger and improved motility in bovine (Sapanidou et al . ) and red deer sperm (Domínguez‐Rebolledo et al . ).…”

Section: Introductionmentioning

confidence: 99%

Bacterioruberin extracts from a genetically modified hyperpigmented Haloferax volcanii strain: antioxidant activity and bioactive properties on sperm cells

et al. 2019

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Aims To examine the antioxidant activity of Bacterioruberin (Bctr)‐rich extracts isolated from a hyperpigmented, genetically modified Haloferax volcanii strain (HVLON3) and to investigate the effect on cold‐sensitive ram sperm cells. Methods and Results The strain HVLON3 produces higher Bctr amounts than most haloarchaea (220 ± 13 mg g−1 DW). HVLON3‐Bctr extract has higher antioxidant activity than β‐carotene (threefold) as evaluated using 2,2 diphenyl‐1‐picrylhydrazyl combined with Electron Paramagnetic Resonance analysis (EC50 4·5 × 10−5 mol l−1 vs 13·9 × 10−5 mol l−1 respectively). Different concentrations of HVLON3‐Bctr extracts were assayed on ram sperm after freezing/thawing and physiologically relevant parameters were examined. Extracts containing 7 and 20 μmol l−1 Bctr significantly improved cell viability (P < 0·0001), total and progressive motility (P < 0·0001) and sperm velocities (P = 0·0172 for curvilinear velocity VCL, P = 0·0268 for average path velocity VAP and P = 0·0181 for straight line velocity VSL) and did not affect other parameters evaluated. Conclusions HVLON3 is an excellent source of natural microbial C50 carotenoids with applicability in Biotechnology, Biomedical and Veterinary fields. HVLON3 Bctr extract improves the quality of cryopreserved ram sperm cells and could be applied to increase insemination yields. Significance and Impact of the Study This study provides an insight on the bioactive properties of a bioproduct derived from haloarchaea (carotenoids) which are so far underexploited.

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Antioxidant effect of crocin on bovine sperm quality and in vitro fertilization

Cited by 69 publications

References 49 publications

Cysteine protects rabbit spermatozoa against reactive oxygen species-induced damages

Cysteine protects rabbit spermatozoa against reactive oxygen species-induced damages

Techniques for sperm evaluation using fluorescent probes

Bacterioruberin extracts from a genetically modified hyperpigmented Haloferax volcanii strain: antioxidant activity and bioactive properties on sperm cells

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