Antioxidant Defense Mechanisms in Murine Epidermis and Dermis and Their Responses to Ultraviolet Light

Shindo, Yasuko; Witt, E; Packer, Lester

doi:10.1111/1523-1747.ep12469048

Cited by 342 publications

(238 citation statements)

References 32 publications

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“…Antioxidant enzymes function cooperatively, and a change in any one of them may affect the equilibrium state of oxidative stress or the levels of reactive oxygen species (ROS). If the ROS remain, without being scavenged in the biological system, they can induce biochemical alterations, including inflammation, oxidation of lipid and protein, DNA damage and activation or inactivation of certain enzymes [38,39,[40][41][42][43]. Thus, the generation of chronic and more intense oxidative stress and inflammatory responses in the obese mice on repeated exposure to UVB irradiation would be anticipated to render them more susceptible to oxidative stress-mediated skin diseases than wild-type mice.…”

Section: Discussionmentioning

confidence: 99%

Obesity increases the risk of UV radiation-induced oxidative stress and activation of MAPK and NF-κB signaling

Katiyar

Meeran

2007

Free Radical Biology and Medicine

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Obesity has been implicated in several diseases including cancer, however, the relationship of obesity and susceptibility to ultraviolet (UV) radiation-caused skin diseases has not been investigated. As UV-induced oxidative stress has been implicated in several skin diseases, we assessed the role of obesity on UVB-induced oxidative stress in genetically obese Lep ob /Lep ob (leptin-deficient) mice. Here, we report that chronic exposure to UVB (120 mJ/cm 2 ) resulted in greater oxidative stress in the skin of obese mice in terms of higher levels of H 2 O 2 and NO production, photo-oxidative damage of lipids and proteins, and greater depletion of antioxidant defense enzymes, like, glutathione, glutathione peroxidase and catalase. As UV-induced oxidative stress mediates activation of MAPK and NF-κB signaling pathways, we determined the effect of UVB on these pathways in obese mice. Exposure of obese mice to UVB resulted in phosphorylation of ERK1/2, JNK and p38 proteins of the MAPK family. Compared to wild-type mice, the obese mice exhibited higher levels of phosphorylation of these proteins, greater activation of NF-κB/p65, and higher levels of circulating proinflammatory cytokines, including TNF-α, IL-1-β and IL-6, on UVB irradiation. Together, our study suggests for the first time that obesity in mice is associated with greater susceptibility to UVBinduced oxidative stress, and therefore may be a risk factor for skin diseases associated with UVBinduced oxidative stress.

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Section: Discussionmentioning

confidence: 99%

Obesity increases the risk of UV radiation-induced oxidative stress and activation of MAPK and NF-κB signaling

Katiyar

Meeran

2007

Free Radical Biology and Medicine

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“…Catalase, SOD, glutathione-peroxidase, glutathione-reductase and intracellular-reduced glutathione work corporately, so that changes in one component can affect the state of balance of ROS. Over-generation of ROS in the biological system can cause cellular damage and biochemical alterations, such as oxidation of proteins and lipids, inflammation, DNA damages, and activation and inactivation of enzymes (Shindo et al 1993). Therefore, the prevention of the production of ROS after UVB irradiation in HaCaT cells (Fig.…”

Section: Discussionmentioning

confidence: 99%

Crude polysaccharide from an anti-UVB cell clone of Bupleurum scorzonerifolium protect HaCaT cells against UVB-induced oxidative stress

et al. 2011

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Bupleurum scorzonerifolium Willd has been found to have a wide range of immunopharmacologic functions. We isolated an anti-UVB B. scorzonerifolium cell clone and found elevated level of polysaccharides. In this study, we investigated the ability of crude polysaccharide (CP) from the anti-UVB B. scorzonerifolium cell clone to inhibit UVBinduced photodamage using a human skin keratinocyte cell line, HaCaT. Cells were UVB irradiated and then incubated in presence of different concentrations of CP. MTT assay showed that the CP did not induce cytotoxic effect under 10 mg/mL and after UVB irradiation, CP can inhibit UVB-induced HaCaT cell death. Decreased reactive oxygen species and lipid peroxidation and increased superoxide dismutase activity showed that CP can act as a free radical scavenger. Furthermore, CP had a strong protective ability against UVB-induced DNA damage. These effects were compared to the crude polysaccharide (CP 0 ) from normal B. scorzonerifolium callus at concentration of 20 mg/mL. The portion of crude polysaccharide (CP) from the anti-UVB B. scorzonerifolium cell clone was more than 2.5-fold higher than crude polysaccharide (CP 0 ) from normal B. scorzonerifolium callus. Taken together, the protective mechanisms of crude polysaccharide from the anti-UVB B. scorzonerifolium cell clone against UVB-induced photodamage occur by the inhibition of UVB-induced reactive oxygen species production, lipid peroxidation and DNA damage.Keywords Anti-UVB Bupleurum scorzonerifolium cell clone Á Crude polysaccharide Á Oxidative stress Á Photodamage Á UVB

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“…The epidermis could be exposed to more foreign oxidant stress than the dermis, because the concentration of antioxidant molecules in the epidermis is higher than in the dermis, and the capacity of antioxidants in the epidermis is greater than that of the dermis [8,37]. VC is an antioxidant molecule [38] and reduces antioxidant stress.…”

Section: Reduce Oxidative Stressmentioning

confidence: 99%

Vitamin C and Skin

Sugiura¹,

Sugiura²

2018

J Clin Exp Dermatol Res

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Vitamin C (VC) is one of nine water-soluble vitamins. VC is known as ascorbic acid (AA), which is a reduced form of VC. AA plays some essential roles in the human body: (1) protecting skin against UV damage; (2) preventing hyperpigmentation of the skin; (3) improving skin inflammation and reducing photocarcinogenesis; (4) increasing collagen fiber; (5) reducing oxidative stress; and (6) immuno-modulating effects. AA deficiency affects collagen and vessels structure, wound healing and hemostasis, and scurvy. VC and AA are essential for life. We hope development of new medicines using VC or AA because of few adverse side effects of VC and AA.

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Antioxidant Defense Mechanisms in Murine Epidermis and Dermis and Their Responses to Ultraviolet Light

Cited by 342 publications

References 32 publications

Obesity increases the risk of UV radiation-induced oxidative stress and activation of MAPK and NF-κB signaling

Obesity increases the risk of UV radiation-induced oxidative stress and activation of MAPK and NF-κB signaling

Crude polysaccharide from an anti-UVB cell clone of Bupleurum scorzonerifolium protect HaCaT cells against UVB-induced oxidative stress

Vitamin C and Skin

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