Antioxidant, Cytotoxic, and Antimicrobial Potential of Silver Nanoparticles Synthesized using Tradescantia pallida Extract

Abstract

Silver nanoparticles have received much attention, due to their wide range of biological applications as an alternative therapy for disease conditions utilizing the nanobiotechnology domain for synthesis. The current study was performed to examine the antioxidant, anticancer, antibacterial, and antifungal potential of biosynthesized silver nanoparticles (TpAgNPs) using plant extract. The TpAgNPs were produced by reacting the Tradescantia pallida extract and AgNO3 solution in nine various concentration ratios subjected to bioactivities profiling. According to the current findings, plant extract comprising phenolics, flavonoids, and especially anthocyanins played a critical role in the production of TpAgNPs. UV–visible spectroscopy also validated the TpAgNP formation in the peak range of 401–441 nm. Further, the silver ion stabilization by phytochemicals, face-centered cubic structure, crystal size, and spherical morphology of TpAgNPs were analyzed by FTIR, XRD, and SEM. Among all TpAgNPs, the biosynthesized TpAgNP6 with a medium concentration ratio (5:10) and the plant extract had effective antioxidant potentials of 77.2 ± 1.0% and 45.1 ± 0.5% free radical scavenging activity, respectively. The cytotoxic activity of TpAgNP6 in comparison to plant extract for the rhabdomyosarcoma cell line was significantly the lowest with IC50 values of 81.5 ± 1.9 and 90.59 ± 1.6 μg/ml and cell viability % of 24.3 ± 1.62 and 27.4 ± 1.05, respectively. The antibacterial and antifungal results of TpAgNPs revealed significant improvement in comparison to plant extract, i.e., minimum inhibition concentration (MIC) 64 μg/ml against Gram-negative Pseudomonas aeruginosa while, in the case of antifungal assay, TpAgNP6 was active against Candida parapsilosis. These TpAgNPs play a crucial role in determining the therapeutic potential of T. pallida due to their biological efficacy.

First identification of potato tuber rot caused by Penicillium solitum, its silver nanoparticles synthesis, characterization and use against harmful pathogens

“…Biogenic Ag NPs penetrate fungus and bacteria cell wall and adhere to cell membrane. These Ag NPs move inside the cell and disrupt metabolic and cellular processes including DNA degradation ( Shahzadi et al., 2022 ). The key mechanism of Ag NPs against bacteria and fungus includes production of reactive oxygen species (ROS) such as hydroxyl radical, superoxide and hydrogen peroxide.…”

“…at 37°C and fungal cultures were incubated for 5 days at 28°C. Antimicrobial activity was determined by measuring the diameter of the zone of inhibition for Ag NPs activity ( Shahzadi et al., 2022 ; Yousaf et al., 2022 ).…”

Bio‐fabrication of silver nanoparticles using an aqueous extract of Quercus baloot: Preparation, characterization and in vitro antimicrobial evaluation

“…Furthermore, the AgNPs may owe their antifungal properties to the presence of bioactive substances, such as anthraquinones, coumarin derivatives and flavonoid glycosides. These substances are known for their antimicrobial activity and may work in combination or synergistically to affect fungi [51].…”

Endophytic fungi of Tradescantia pallida mediated targeting of Multi-Drug resistant human pathogens

“…TpAgNPs revealed significant antibacterial activity against Pseudomonas aeruginosa with MIC of 64 μg/mL and antifungal activity against Candida parapsilosis . Further, the plant extract had effective antioxidant potentials of 77.2 ± 1.0 % and 45.1 ± 0.5 % free radical scavenging activity ( Shahzadi et al, 2022 ). In Malaysia, the Ayta communities of Porac Pampanga utilize this plant to treat sore eyes ( Ragragio et al, 2013 ).…”