Antioxidant Constituents in the Fruits of Luffa cylindrica (L.) Roem

Abstract

Hydrophilic antioxidant constituents in the fruits of the vegetable Luffa cylindrica (L.) Roem (sponge gourds) were separated by an antioxidant-guided assay to yield eight compounds: p-coumaric acid (1), 1-O-feruloyl-beta-D-glucose (2), 1-O-p-coumaroyl-beta-D-glucose (3), 1-O-caffeoyl-beta-D-glucose (4), 1-O-(4-hydroxybenzoyl)glucose (5), diosmetin-7-O-beta-D-glucuronide methyl ester (6), apigenin-7-O-beta-D-glucuronide methyl ester (7), and luteolin-7-O-beta-D-glucuronide methyl ester (8). The eight compounds were isolated by high-speed countercurrent chromatography and identified by electrospray ionization-mass spectrometry and NMR analysis, and the antioxidant activity was evaluated by the radical scavenging effect on the 1,1-diphenyl-2-picrylhydrazyl radical. High-performance liquid chromatography analysis showed that a total amount of the eight compounds in the dried gourds without skin was about 1%. The results demonstrate that the consumption of sponge gourds can supply some antioxidant constituents to human body.

Protective effects of luteolin-7-O-β-D-glucuronide methyl ester from the ethyl acetate fraction of Lycopi Herba against pro-oxidant reactive species and low-density lipoprotein peroxidation

“…The spectral data for compound 1 was in good agreement with those previously reported in the literature [16]. The antioxidant activities of LGME showed significant differences (p < 0.05) and their TEAC value was 1.990 mmol Trolox equivalent units.…”

Preparative Separation of Phenolic Constituents in the Fruits of Luffa cylindrica (L.) Roem using Slow Rotary Countercurrent Chromatography

“…The solvent systems were the same as those for HSCCC separation. [2] The solvent systems of chloroform-methanolisopropanol-water (5:6:1:4. v/v) and chloroform-methanol-water (13:7:8, v/v) were used for the separation of Fr30 and Fr50, respectively, with aqueous upper phase as the stationary phase. The sample solutions were prepared by dissolving 3.0 g each of Fr30 and Fr50 with 300 mL of corresponding mobile phases, respectively.…”

“…It is also covered in the traditional Chinese medicine (TCM) as an anthelmintic, stomachic, and antipyretic phytomedicinal drug. [1] By employing high speed countercurrent chromatography (HSCCC), phenolic compounds including flavonoid glycosides were separated from sponge gourd extract pre-purified by using macro-porous resin AB-8 [2] for structure identification of the compounds. A larger amount of the compounds are expected for advanced bioactive studies.…”

Excellent combination of HPLC-RSD-DAD-ESI/MS and HSCCC experiments to screen and identify radical scavengers from Neo-Taraxacum siphonanthun

“…This method has been successfully applied to separate and isolate active components from natural products. 14,15 500 mg of the active extract from F 3 fraction of Neo-T. siphonanthun extract was performed by HSCCC with a biphasic solvent system composed of n-hexane-n-butanolwater (1:1:2, v/v) using the upper phase as the stationary phase. Three fractions (1-3) were obtained in one-step elution less then 6 h as shown in Figure 4a, which is 26.3 mg of fraction 1 (collected during 145-180 min), 7.8 mg of fraction 2 (collected during 200-225 min) and 49.5 mg …”