Antioxidant compounds in the seaweedGelidiella acerosaprotects human Peripheral Blood Mononuclear Cells against TCDD induced toxicity

Abstract

2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is a persistent environmental toxin formed as an unintentional by-product of incomplete combustion. Several therapeutic approaches have evolved to combat its toxicity since it elicits immunotoxicity, neurotoxicity, hepatotoxicity, carcinogenicity and lethality. Search for drugs from natural resources especially from seaweeds has become intense due to their enormous pharmacological potential. Hence, the present study aims at revealing the protective effect of methanolic extract of G. acerosa (MEGA) in Peripheral Blood Mononuclear Cells (PBMC) against TCDD induced toxicity, by assessing the antioxidant, anti-apoptotic and cytoprotective activities. The results of antioxidant assays suggests that MEGA reverted TCDD induced toxicity by causing an alteration in the levels of antioxidant enzymes (Catalase [CAT], Superoxide dismutase [SOD], Glutathione peroxidase [GPx], Glutathione-S-transferase [GST]) and Glutathione [GSH]. The results of lipid peroxidation assay and protein carbonyl content reveal that MEGA protects PBMC from TCDD induced macromolecular damage. MEGA was found to exhibit significant (p < 0.05) anti-apoptotic activity as verified by evaluation of mitochondrial membrane potential and AO-EtBr dual staining. In addition, PBMC co-treated with MEGA prevented TCDD induced oxidative DNA damage. Levels of phase-I detoxification enzymes determined by EROD assay and semi-quantitative RT-PCR showed that TCDD up-regulates the expression of CYP1A1 and upon co-treatment with MEGA, the expression got slightly decreased suggesting its protective role. Preliminary phytochemical analysis demonstrates that the extract is rich in cardiac glycosides and terpenoids. LC-MS analysis revealed the presence of antioxidants including caffeic acid, phytol and mannoheptulose in MEGA, which could be attributed for the observed protective effect against TCDD induced toxicity.

Gelidiella Acerosacompounds Target NFKB Cascade in Lung Adenocarcinoma

“…The outcomes of the current study are in line with earlier studies where G.acerosawas shown to regulate SOD activity in Alzheimer's disease [28] and to protect human peripheral mononuclear cells from TDCCinduced toxicity [29]. As excess ROS are associated with cellular damage, in ammation and cancer progression, the enhanced activity of antioxidant enzymes may confer protection against free radical damage to cells [30].…”

Catalpol protects against 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin‐induced cytotoxicity in osteoblastic MC3T3‐E1 cells

“…Compared with the TCDD-treated group, the gene expression levels of ALP and osterix were enhanced by 1 μM catalpol.4 | DISCUSSIONExposure to TCDD, a persistent organic pollutant, causes the formation of reactive oxygen species (ROS) and induces oxidative stress that results in toxicity(Mates, Segura, Alonso, & Marquez, 2010). Previous studies have emphasized the roles that natural antioxidants, particularly those derived from medicinal plants, play in the prevention of TCDD toxicities(Du et al, 2017;Ilavarasi et al, 2015). For example, recent studies by our research group have shown that various flavonoids, including actein(Choi et al, 2017), 27-deoxyactein(Suh, Choi, Jung, et al, 2018) and xanthohumol(Suh, Choi, Kim, et al, 2018), inhibit TCDD-induced toxicity in MC3T3-E1 osteoblastic cells.…”

Chemopreventive effect of natural dietary compounds on xenobiotic-induced toxicity

“…Sandur et al [ 81 ] found that natural curcuminoid extracts from Curcuma longa exhibited more anti-inflammatory and antiproliferative activities than pure curcumin, curcumin derivatives and reconstituted curcuminoids. Recently, Suwannakul et al [ 82 ] and Ilavarasi et al [ 83 ] reported that extracts of purple rice bran and seaweed had the ability to attenuate the ABF1-induced initiation stage of hepatocarcinogenesis through alteration of XMEs and blockage of TCDD-induced toxicity, respectively.…”