Antioxidant capacity of the striped sunflower (Helianthus annuusL.) seed extracts evaluated by threein vitromethods

Abstract

The antioxidant capacity of the striped sunflower seed cotyledon extracts, obtained by sequential extraction with different polarities of solvents, was evaluated by three different in vitro methods: ferric reducing/antioxidant power (FRAP), 2.2-diphenyl-1-picrylhydrazyl radical (DPPH) and oxygen radical absorbance capacity (ORAC) assays. In the three methods, the aqueous extract at 30 µg/ml showed a higher antioxidant capacity value (FRAP, 45.27 µmol; DPPH, 50.18%; ORAC, 1.5 Trolox equivalents) than the ethanolic extract (FRAP, 32.17 µmol; DPPH, 15.21%;ORAC, 0.50 Trolox equivalents). When compared with the synthetic antioxidant butylated hydroxyl toluene, the antioxidant capacity of the aqueous extract varied from 45% to 66%, according to the used method. The high antioxidant capacity observed for the aqueous extract of the studied sunflower seed suggests that the intake of this seed may prevent in vivo oxidative reactions responsible for the development of several diseases, such as cancer.

Food Phenolic Compounds: Main Classes, Sources and Their Antioxidant Power

“…It is also know that tamarind, canola, sesame, linseed and sunflower seeds are other possible sources of phenolic compounds [73] and have high antioxidant capacity. The antioxidant capacity of the striped sunflower seed cotyledon extracts, obtained by sequential extraction with different polarities of solvents, was determined by three in vitro methods: FRAP, DPPH and ORAC [74]. In the three methods applied, the aqueous extract showed higher antioxidant capacity than the ethanolic.…”

Uma Abordagem Sobre a Capacidade Antioxidante in Vitro De Alimentos Vegetais E Bebidas

“…15 Assim, em qualquer ensaio de capacidade antioxidante, os resultados devem ser medidos em extratos ou soluções, bem como relacionados a uma curva de calibração, no mesmo solvente. Em nossos estudos, em função da característica das amostras e antioxidantes naturais estudados, adotamos a extração sequencial com solventes de diferentes polaridades 16 (figura 3).…”

Ação antioxidante de extratos de especiarias e suas misturas binárias e ternárias sobre a estabilidade oxidativa de óleo de soja

“…Os resultados do poder antioxidante de redução do ferro podem ser expressos como equivalente em micromoles de Trolox/grama de amostra (em base seca) ou equivalente em milimols de Fe 2+ /Kg. É um método rápido, simples, com resultados reprodutíveis, sua única desvantagem é que o sistema deve ser aquoso(MOON;SHIBAMOTO, 2009; Mancini-Filho (2009). compararam os métodos FRAP, DPPH e ORAC e observaram que eles se correlacionaram de forma positiva.…”