Antioxidant capacity of plant raw material of Scutellaria baicalensis Georgi

Abstract

The aim of this study was to evaluate antioxidant capacity of Scutellaria baicalensis Georgi from two regions of Ukraine: Kyiv city (M. M. Gryshko National Botanical Garden of NAS of Ukraine (NBG)) and Kherson region (Experimental Facility “Novokakhovska” of Rice Research Institute of Ukrainian Academy of Agrarian Sciences (EFN of RRI)). Observation of plants and biochemical analyses conducted with plants collected in the stage of flowering. In study investigated and compared above-ground part of plants and separated organs: inflorescences, stems, leaves. Measured morphometric parameters (height of plants, length, and width of leaves, length, and diameter of inflorescence, the diameter of the stem) showed that the most variable was the length of inflorescence (12.79%) for NBG sample and diameter of the stem (33.33%) for EFN of RRI sample. Ethanolic extracts were screened for the antioxidant capacity. As standards were used gallic acid for polyphenol content (GAE), quercetin for flavonoids (QE), caffeic acid for phenolic acids (CAE), Trolox for antioxidant capacity (TE). The total content of polyphenol compounds was 42.43 – 86.13 mg GAE.g-1 DW (dry weight) (NBG sample) and 28.06 – 96.76 mg GAE.g-1 DW (EFN of RRI sample). The content of flavonoids was 9.39 – 62.97 mg QE.g-1 DW (NBG sample) and 10.64 – 66.07 mg QE.g-1 DW (EFN of RRI sample). The concentration of phenolic acids was 2.60 – 16.13 mg CA.g-1 DW (NBG sample) and 12.02 – 30.12 CA.g-1 DW (EFN of RRI sample). Antioxidant activity of plant extracts was measured by DPPH assay and reducing power method. The first method indicated an antioxidant ability 8.24 – 8.56 mg TE.g-1 DW (NBG sample) and 7.63 – 8.83 mg TE.g-1 DW (EFN of RRI sample). Reducing power of extracts was 51.48 – 306.09 mg TE.g-1 DW (NBG sample) and 63.33 – 260.24 mg TE.g-1 DW (EFN of RRI sample). Very strong positive correlation identified between total polyphenol content, total flavonoid content and reducing power. Scutellaria baicalensis is a rich source of antioxidants and potential raw of further pharmacological study in Ukraine as well as in other regions for improving and enrichment of relevant production.

Biomedical Applications of Scutellaria edelbergii Rech. f.: In Vitro and In Vivo Approach

“…Among the investigated fractions against DPPH and ABTS, chloroform fraction with IC 50 value of 175 and 155 µg/mL was the most potent, followed by EtOAc with IC 50 values of 205 and 160 µg/mL. Our results match with reported data of Vergun et al [ 31 ], who studied Scutellaria baicalensis in the same fractions and concentrations against DPPH radical. Our study is also in agreement with the study of Grzegorczyk-Karolak et al [ 32 ], Delazar et al [ 33 ] and Zengin et al, [ 34 ] who have performed the same tests for S. altissima and S. pinnatifida , S. Orientalis L .…”

Antioxidant Activity of Extracts of Wild Humulus lupulus L.

“…It should be noted that the least content of phenolic acids found in the stems and the most in the flowers. The same results were found in different organ extracts of Scutellaria baicalensis and Galega spp., where the lowest content of phenolic acids found in stems at the flowering (Vergun et al, 2019(Vergun et al, , 2020.…”

Scutellaria baicalensis – a small plant with large pro-health biological activities

“…Similar activity was observed for baicalein, baicalin and scutellarin when analyzed via DPPH, ABTS and CAA assays. The three flavonoids made contributions of 58.33%, 60.36% and 51.41% (respectively) to the overall antioxidant activity of a flavonoid-rich extract [40]. Of note, a study of the comparison of the antioxidant activity of extracts obtained from root, leaf, stem and flower performed by Seo et al revealed high antioxidant activity for all used methods (DPPH, ABTS and RP), nevertheless, the highest activity was observed for root, followed by leaf, stem and flower [41].…”