Antioxidant capacity of flavonoids from Folium Artemisiae Argyi and the molecular mechanism in Caenorhabditis elegans

Hu, Qian; Liu, Zegan; Guo, Yujie; Lu, Shan; Cao, Yanjun

doi:10.1016/j.jep.2021.114398

Cited by 59 publications

(48 citation statements)

References 21 publications

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“…SOD and CAT are considered to be the main antioxidant enzymes that maintain the balance of oxidation and antioxidant in vivo [ 26 ]. Most flavonoids reduce free radicals as hydrogen donors, thereby attenuating peroxidative damage and enhancing antioxidant capacity, due to their varying content of phenolic hydroxyl groups [ 27 ]. A previous study reported that PT obviously alleviated S. Typhimurium -induced oxidative stress in the colon by suppressing the level of MDA and increasing the vitality of GSH-PX and SOD [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

Phloretin Protects Bovine Rumen Epithelial Cells from LPS-Induced Injury

Wang

Lei

et al. 2022

Toxins

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Lipopolysaccharide (LPS) is an endotoxin that induces immune and inflammatory responses in the rumen epithelium of dairy cows. It is well-known that flavonoid phloretin (PT) exhibits anti-oxidative, anti-inflammatory and antibacterial activity. The aim of this research was to explore whether PT could decrease LPS-induced damage to bovine rumen epithelial cells (BRECs) and its molecular mechanisms of potential protective efficacy. BRECs were pretreated with PT for 2 h and then stimulated with LPS for the assessment of various response indicators. The results showed that 100 µM PT had no significant effect on the viability of 10 µg/mL LPS-induced BRECs, and this dose was used in follow-up studies. The results showed that PT pre-relieved the decline in LPS-induced antioxidant indicators (T-AOC and GSH-PX). PT pretreatment resulted in decreased interleukin-1β (IL-1β), IL-6, IL-8, tumor necrosis factor-α (TNF-α) and chemokines (CCL2, CCL5, CCL20) expression. The underlying mechanisms explored reveal that PT may contribute to inflammatory responses by regulating Toll-like receptor 4 (TLR4), nuclear transcription factor-κB p65 (NF-κB p65), and ERK1/2 (p42/44) signaling pathways. Moreover, further studies found that LPS-induced BRECs showed decreased expression of claudin-related genes (ZO-1, Occludin); these were attenuated by pretreatment with PT. These results suggest that PT enhances the antioxidant properties of BRECs during inflammation, reduces gene expression of pro-inflammatory cytokines and chemokines, and enhances barrier function. Overall, the results suggest that PT (at least in vitro) offers some protective effect against LPS-induced ruminal epithelial inflammation. Further in vivo studies should be conducted to identify strategies for the prevention and amelioration of short acute rumen acidosis (SARA) in dairy cows using PT.

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Section: Discussionmentioning

confidence: 99%

Phloretin Protects Bovine Rumen Epithelial Cells from LPS-Induced Injury

Wang

Lei

et al. 2022

Toxins

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“…The CAT can decompose harmful hydrogen peroxide. Hu et al ( 41 ) demonstrated that flavonoids could improve antioxidant capacity via the increase of gene expression levels related to antioxidation such as SOD-3.…”

Section: Discussionmentioning

confidence: 99%

Flavonoids from Lycium barbarum leaves attenuate obesity through modulating glycolipid levels, oxidative stress, and gut bacterial composition in high-fat diet-fed mice

et al. 2022

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Traditional herbal therapy made from Lycium barbarum leaves has been said to be effective in treating metabolic diseases, while its exact processes are yet unknown. Natural flavonoids are considered as a secure and reliable method for treating obesity. We thus made an effort to investigate the processes by which flavonoids from L. barbarum leaves (LBLF) reduce obesity. To assess the effectiveness of the intervention following intragastric injection of various dosages of LBLF (50, 100, and 200 mg/kg⋅bw), obese model mice developed via a high-fat diet were utilized. Treatment for LBLF may decrease body weight gain, Lee’s index, serum lipids levels, oxidative stress levels, and hepatic lipids levels. It may also enhance fecal lipids excretion and improve glucose tolerance. Additionally, LBLF therapy significantly restored gut dysfunction brought on by a high-fat diet by boosting gut bacterial diversities and altering the composition of the gut bacterial community by elevating probiotics and reducing harmful bacteria.

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“…According to the methods in recent literature [ 20 , 21 ], the hydroxyl radical scavenging test steps are as follows: 1 mL of cotton flowers flavonoid solution of different concentrations was put into a 10 mL test tube, 1 mL of 6 mmol/L FeSO 4 was added then, followed by 1 mL of 6 mmol/L H 2 O 2 . After 10 min, 1 mL of 6 mmol/L ethanol-salicylic acid solution was mixed with the previous mixed solution at 37 °C for 30 min.…”

Section: Methodsmentioning

confidence: 99%

Effects of Extraction Technique on the Content and Antioxidant Activity of Flavonoids from Gossypium Hirsutum linn. Flowers

Dong

Zhou

et al. 2022

Molecules

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Cotton is one of the Uyghur medical materials in China and is rich in flavonoids. Flavonoids have important pharmacological effects. The yield of flavonoids in traditional extraction methods is low, which affects the development of flavonoids. Therefore, it is urgent to optimize the extraction techniques. The yield of flavonoids in cotton flowers was effectively improved by response surface methodology, and the highest yield of flavonoids reached 5.66%, and the optimal extraction process conditions were obtained. The DPPH free radical scavenging rate, hydroxyl free radical scavenging rate, superoxide anion free radical scavenging rate, and reducing ability were tested to reflect the antioxidant capacity of flavonoids. The flavonoids had an excellent antioxidant effect. Cell experiments suggested that the flavonoids had the effect of protecting glutamate-induced damage to HT-22 cells. The results of this study provide a theoretical basis for the extraction of cotton flowers flavonoids and the comprehensive evaluation of antioxidant products, as well as the extraction of other plant flavonoids.

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Antioxidant capacity of flavonoids from Folium Artemisiae Argyi and the molecular mechanism in Caenorhabditis elegans

Cited by 59 publications

References 21 publications

Phloretin Protects Bovine Rumen Epithelial Cells from LPS-Induced Injury

Phloretin Protects Bovine Rumen Epithelial Cells from LPS-Induced Injury

Flavonoids from Lycium barbarum leaves attenuate obesity through modulating glycolipid levels, oxidative stress, and gut bacterial composition in high-fat diet-fed mice

Effects of Extraction Technique on the Content and Antioxidant Activity of Flavonoids from Gossypium Hirsutum linn. Flowers

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