Antioxidant capacity of Dillenia sp. leaf extract against DPPH (1,1-Diphenyl-2picryl Hidrazil) radical

Abstract

Some plants have capacity as exogenous antioxidant. Simpor (Dillenia sp.) is plant of the Dilleniaceae family which may have the property of antioxidant. The purpose of this study was to measure the antioxidant capacity of the 96% ethanolic simpor leaf extract. Factorial design was applied with 2x3 factors of maseration time (24 and 48 hours) and concentration series of (10, 15, 20%). Normality was done by Kolmogorov-Smirnov and homogeneity by Levine. Data was analysed by ANOVA (ᾳ = 0.05). Post Hoc was analysed by Duncan. Antioxidant property was analysed qualitatively and quantitatively on flavonoid and also leaves water content. The antioxidant capacity assay was measured using DPPH (1,1-Diphenyl-2picryl Hidrazil) scavenging ability at 517 nm with ascorbic acid as control. The result showed that water content of the plant was 73.65%. Simpor contain flavonoids with highest flavonoid content (2.953 mg QE / mg) in 24 hours maceration time at 15% concentration. Furthermore, it also has ability to scavenge DPPH (1,1-Diphenyl-2picryl Hidrazil) radical with the highest value (97,10 ± 0,01%) in 24 and 48 hours maceration at 20% and 10% extract concentration. Statistical analysis on flavonoid content showed that time and concentration gave significant effect on flavonoid content (ᾳ = 0.003). Duncan result revealed that almost all groups didn’t significantly different only the 24 hours x 15% concentration was. DPPH inhibition based on statistic proved that both factors didn’t affect scavenging ability of Dillenia (ᾳ = 0.079). It can be concluded that simpor leaves has antioxidant capacity against DPPH radical due to its flavonoid content. Furthermore, concentration and maceration time significantly affect flavonoid content and didn’t affect DPPH radical scavenging ability of Dillenia plant.

Antioxidant and Anticancer Activity of Dillenia serrata Thunb Ethanol Extract Against MCF-7 Breast Cancer Cell Line

“…The result showed that the ethanol extract of D. serrata leaves had better antioxidant activity than other extracts with a strong level of antioxidant activity. This is in accordance with what was stated by Rahayu, et al (2019), most of the flavonoids and phenols were found in the leaves and have the potential as a source of antioxidants, and are also supported by a study from Sabandar, et al (2017), quercetin compounds are found in the leaves of all types of Dillenia sp. Quercetin is a type of flavonoid antioxidant compound contained in plant foods.…”

Phytochemical screening and antioxidant activity of methanol extract of Dillenia excelsa leaf

“…In comparison, at the lowest concentration of 6.25 ppm, the percentage inhibition was 71.461%, radical inhibitory activity was characterized by increasing sample concentration, and the high concentration of D. excelsa leaf methanol extract, which is 100 ppm, indicates that the content of active antioxidant compounds is more so that the ability to inhibit free radicals will increase. Ethanol extract of D. indica has an inhibition percentage of 97.10±0.01% (Saha et al 2009;Rahayu et al 2019). Ethanolic extract of D. bracteata leaves inhibited 74% with DPPH (Shama et al 2019).…”

“…The solution of D. excelsa leaf methanol extract was prepared by weighing 5 mg and diluting with 10 mL of ethanol so that a concentration of 500 µg/mL was formed; variations in concentration were made, namely 200, 100, 50, 25, 12.5, and 6.25 ppm, each concentration was pipetted. 33 µL, added 467 µL of ethanol and 500 mL of 27% DPPH solution, incubated for 20 minutes in a dark room, and measured absorbance using a UV-Vis spectrophotometer with a wavelength of 517 nm (Rahayu et al 2019) The percentage of antioxidant activity was calculated using the following equation:…”

“…Light, temperature, drought, and salinity affect plants' secondary metabolite content. Rahayu et al (2019) reported that ethanol extract of D. indica leaves contains a flavonoid content of 2.953 mg (QE/mg) in macerated 24 hours at 15% concentration. Ethanolic extract of D. bracteata leaves has 61.67 mg quercetin equivalents per g of dry extract (Shama et al 2019).…”

“…Study using Dillenia indica samples with positive phytochemical test results containing alkaloids, phenols, tannins, flavonoids, steroids, and triterpenoids, as well as saponins (Shipra et al 2018;Bordoloi et al 2019;Prananda et al 2019;Nkop et al 2020), antimicrobial, cytotoxicity, and sedative activity (Akter et al 2021;Lashin et al 2022), the fruit powder potential antidiabetic (Kamboj et al 2019), also make them pharmaceutically useful (Bose et al 2010;Kumar et al 2018). Dillenia suffruticosa ethyl acetate extract showed that the positive sample contained secondary metabolites of tannins, polyphenols, and triterpenoids (Putra et al 2018;Rahayu et al 2019;Yacop et al 2020;Syafriana et al 2021). Dillenia serrata has the potential as an antioxidant prospect to be used in herbal and drug development from nature (Sabandar et al 2020).…”