Leaves and drupes of the major Greek olive varieties 'Koroneiki', 'Lianolia Kerkyras', 'Mastoidis', 'Adramytini', 'Megaritiki', 'Gaidourelia', 'Kalamata', 'Konservolia', 'Chalkidiki' and the Spanish variety 'Arbequina' were collected at different developmental stages during two consecutive years and investigated by HPLC for their phenolic profile and the concentration of the phenolic compounds present. The phenolic compounds identified in year 1 new season leaves were, in declining concentration order, oleuropein, 7-O-glucoside of luteolin and rutin, whereas for those collected in year 2 the main phenolic compounds were oleuropein, rutin, 4-O-glucoside of luteolin and 7-O-glucoside of apigenin. In SeptemberDecember collected leaves of year 2, oleuropein presented the higher concentration followed by 7-O-glucoside of luteolin, 4-O-glucoside of luteolin and rutin. Regarding green and black drupes for both years, the main phenolic compounds were oleuropein, verbascoside and rutin. Verbascoside was only found in drupes whereas the 7-O-glucoside of apigenin only in leaves. The concentration of oleuropein showed high fluctuations both between different tissues within the same variety and between different varieties within the same tissue. The number of phenolic compounds identified in the phenolic profile of green and black drupes was lower than that of leaves regardless developmental stage or year. Differences were observed in the concentration of phenolic compounds depending on the variety in all tissues. The phenolic profile of new season leaves, green and black drupes was similar for both years.