Antioxidant capacity and hepatoprotective activity of myristic acid acylated derivative of phloridzin

Abstract

The antioxidant activities in vitro and hepatoprotective effects against carbon tetrachloride (CCl 4 ) induced acute liver injury in vivo of myristic acid acylated derivative of phloridzin (PZM) were investigated. The PZM was obtained by enzymatic acylation of myristic acid and phloridzin (PZ). The antioxidant capability of PZM in vitro was evaluated by the ferric reducing antioxidant power assay (FRAP), 2,2′-Azinobis- 3-ethylbenzthiazoline-6-sulphonate (ABTS + ·) and 2,2-diphenyl-1-picrylhydrazyl (DPPH·) radical scavenging assay. Mice were intragastrically treated with control or PZM (20, 40, and 80 mg/kg) for 5 days and intra-peritoneal injection with CCl 4 . The enzymatic acylated synthesis of myristic acid and phloridzin was region-selective taken place on 6″-OH of phloridzin glycoside moiety and achieved 93% yield. PZM had a significantly higher total antioxidant ability, same scavenging ABTS + · ability and weaker scavenging DPPH· ability when compared to the parent PZ. The of aminotransferase serum activity and malondialdehyde hepatic activity were elevated (P < 0.015) after treatment with CCl 4 , while the related liver enzymatic activities and glutathione concentration were lower. These changes were enhanced by PZM. Further studies showed that PZM reduced the interleukin-6 expression and stimulated liver regeneration caused by CCl 4 . PZM attained good antioxidant capacity in vitro and had excellent hepatoprotective effects in vivo and better bioactivity compared to the parent phloridzin. The significance of hepatoprotective effect of phloridzin derivative against CCl 4 -induced hepatotoxicity in mice is an important and new finding.

Laccase Catalyzed Oxidative Polymerization of Phloridzin: Polymer Characterization, Antioxidant Capacity and α-Glucosidase Inhibitory Activity

“…Phloridzin is a natural dihydrochalcone with various pharmacological effects, such as antioxidant, anti-inflammatory, anti-viral, antihypertensive, antihyperglycemic and anti-tumor activities. [1][2][3] The compound is found in many plants, particularly Malus species, being an active ingredient in herbal medicines. 4,5 However, its low aqueous solubility and poor enzyme inhibitory capacity have limited its application in functional foods and medicines.…”

New insight on antioxidants and anti-obesity properties of two seagrasses Thalassia hemprichii and Zostera marina: an integrated molecular docking simulation with in vitro study

“…66 Rosmarinic acid, alpha-eleostearic acid, p-coumaric acid, myristic acid, diosmetin, and azelaic acid have also shown antioxidant activity based on several previous studies. [67][68][69][70][71][72] This study is the first to successfully profile metabolites and perform molecular docking, and the results are promising for anti-obesity and antioxidant functional foods based on in vitro validation and molecular docking results. However, this still needs further research i.e., isolation and purification of each observed compound and continuing in vivo trials in animal models prior to human clinical trials.…”

Protective Effect of Apple Polyphenols on H₂O₂-Induced Oxidative Stress Damage in Human Colon Adenocarcinoma Caco-2 Cells

“…42) Moreover, myristic acid acylated derivative of phloridzin, which mainly occurred on the 6′-OH of phloridzin glycoside, was also demonstrated to have significantly higher total antioxidant activity than the parent phloridzin both in vitro and in vivo. 43) Thus it is speculated that the chlorogenic acid, procyanidin, epicatechin and phloridzin in AP all contributed to its strong antioxidant activities. In our study, it could be found that adding different concentrations of AP 24 h before H 2 O 2 treatment could effectively improve the activity of Caco-2 cells, maintain the cell morphology and significantly increase the number of viable cells (p < 0.05).…”