Antioxidant capacity and cytotoxic effect of an optimized extract of isabella grape (Vitis labrusca) on breast cancer cells

Vélez, M.  Daniela; Llano-Ramírez, María Alejandra; Ramón-Palacio, Carolina; Rojas, J. A.; Bedoya, Carolina; Arango-Varela, Sandra; Santa-González, Gloria A.; Garzón, Maritza Andrea Gil

doi:10.1016/j.heliyon.2023.e16540

Cited by 2 publications

(2 citation statements)

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“…MCF-10A normal breast cells, which we found not being affected by E7 treatment (Figure 1), are also ER-beta-negative [50]. In a similar study investigating the cytotoxic effects of Vitis Labrusca extract on breast cancer cells, MCF-7 cells were also sensitive to treatment, whereas triple-negative MDA-MB-231 cells had no response to treatment [44]. Polyphenols, the main bioactive component of grape seeds extract, are known to bind to ERα and ERβ, and to exert properties that either mimic or antagonize the action of endogenous estrogens, even at low concentrations [51].…”

Section: Discussionsupporting

confidence: 53%

“…In a study aimed to optimize the extraction methodology in Isabella grape (Vitis labrusca), the resulting extract showed antioxidant capacity and cytotoxicity in MCF-7 breast cancer cells. The TPC expressed as milligrams of gallic acid equivalents per gram of sample (mg GAE/g sample) was 43.14 ± 5.00 mg [44]. Overall, aqueous methanol has proved an effective solvent for isolation of total phenolics and flavonoids from Vitis vinifera leaves as well as for increased radical scavenging and antioxidant activities [26].…”

Section: Discussionmentioning

confidence: 95%

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Antioxidant and Anticancer Activity of Vitis vinifera Extracts in Breast Cell Lines

Tsantila,

Esslinger,

Christou

et al. 2024

Life

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Vitis vinifera extracts have been shown to possess antioxidant activity because of their polyphenol content. In addition, their therapeutic potential against several diseases, including cancer, has been reported. In this study, we produced twelve extracts from the seeds, fruit, leaves, and wood of the Vitis vinifera Airen variety using different extraction methodologies and measured their total polyphenol content (TPC). We also determined their antioxidant and antiproliferative effects against normal cells and evaluated the most potent extract against a panel of breast cancer cell lines. We found that the extracts produced by the seeds of Vitis vinifera had a higher TPC compared to the other parts of the plant. Most extracts produced from seeds had antioxidant activity and did not show cytotoxicity against normal breast cells. The extract produced from whole organic seeds of white grape showed the best correlation between the dose and the ROS inhibition at all time points compared to the other seed extracts and also had antiproliferative properties in estrogen-receptor-positive MCF-7 breast cancer cells. Its mechanism of action involves inhibition of proteins Bcl-2, Bcl-xL, and survivin, and induction of apoptosis. Further investigation of the constituents and activity of Vitis vinifera extracts may reveal potential pharmacological applications of this plant.

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Section: Discussionsupporting

confidence: 53%

Section: Discussionmentioning

confidence: 95%