Antioxidant capacities of the selenium nanoparticles stabilized by chitosan

Abstract

BackgroundsSelenium (Se) as one of the essential trace elements for human plays an important role in the oxidation reduction system. But the high toxicity of Se limits its application. In this case, the element Se with zero oxidation state (Se0) has captured our attention because of its low toxicity and excellent bioavailability. However, Se0 is very unstable and easily changes into the inactive form. By now many efforts have been done to protect its stability. And this work was conducted to explore the antioxidant capacities of the stable Se0 nanoparticles (SeNPs) stabilized using chitosan (CS) with different molecular weights (Mws) (CS-SeNPs).ResultsThe different Mws CS-SeNPs could form uniform sphere particles with a size of about 103 nm after 30 days. The antioxidant tests of the DPPH, ABTS, and lipid peroxide models showed that these CS-SeNPs could scavenge free radicals at different levels. And the 1 month old SeNPs held the higher ABTS scavenging ability that the value could reach up to 87.45 ± 7.63% and 89.44 ± 5.03% of CS(l)-SeNPs and CS(h)-SeNPs, respectively. In the cell test using BABLC-3T3 or Caco-2, the production of the intracellular reactive oxygen species (ROS) could be inhibited in a Se concentration-dependent manner. The topical or oral administration of CS-SeNPs, particularly the Se nanoparticles stabilized with low molecular weight CS, CS(l)-SeNPs, and treated with a 30-day storage process, could efficiently protect glutathione peroxidase (GPx) activity and prevent the lipofusin formation induced by UV-radiation or d-galactose in mice, respectively. Such effects were more evident in viscera than in skin. The acute toxicity of CS(l)-SeNPs was tenfold lower than that of H2SeO3.ConclusionsOur work could demonstrate the CS-SeNPs hold a lower toxicity and a 30-day storage process could enhance the antioxidant capacities. All CS-SeNPs could penetrate the tissues and perform their antioxidant effects, especially the CS(l)-SeNPs in mice models. What’s more, the antioxidant capacities of CS-SeNPs were more evident in viscera than in skin.

Folate-Targeted mRNA Delivery Using Chitosan-Functionalized Selenium Nanoparticles: Potential in Cancer Immunotherapy

“…The hydroxyl groups of chitosan reacted with SeO 3 2− , which was then reduced to SeNPs by ascorbic acid [28]. Previous studies have demonstrated successful chitosan encapsulation of SeNPs, which increased cellular retention of the nanoparticles [29,30]. The SeNPs were predominantly spherical in shape, as seen under transmission electron microscopy (TEM) ( Figure 1), with an average size of 85.3 ± 8 nm and a negative zeta (ζ) potential (−14.8 ± −3.6 mV) ( Table 1).…”

Synergistic antifungal effect of chitosan-stabilized selenium nanoparticles synthesized by pulsed laser ablation in liquids against <em>Candida albicans</em> biofilms

“…25 Therefore, SeNPs have demonstrated an anti-Candida biofilm effect. 19 cs Another compound tested in this study alone or in combination with SeNPs was CS, an effective capping and stabilizing agent of SeNPs 28,29 with fungicidal effects. [30][31][32] CS is a polysaccharide derivative of chitin, extracted from fungi, arthropods such as crustaceans and insects.…”

“…30,34 CS-decorated SeNPs (CS-SeNPs) synthesized by chemical methods have been previously studied in cancer research. 28,29…”

Interaction of γ-Fe2O3 nanoparticles with Citrus maxima leaves and the corresponding physiological effects via foliar application

“…Nowadays, nanomaterials become a hotspot of research interests and attract the attention of many researchers. A variety of nanoparticles (NPs) have been studied on human cells [4, 5], animal cells [6] and plants [7] about their toxicity or applications. As one of the most widely explored and applied nanomaterials, iron oxide nanoparticles (γ-Fe 2 O 3 NPs) are widely used in medical diagnostics, controlled drug release, separation technologies and environmental engineering [8].…”