Antioxidant Biomarkers in Freshwater Bivalves, Unio tumidus, in Response to Different Contamination Profiles of Aquatic Sediments

Cossu, Costanza; Doyotte, A; Babut, Marc; Exinger, A.; Vasseur, Paule

doi:10.1006/eesa.1999.1842

Cited by 213 publications

(76 citation statements)

References 55 publications

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“…This, in turn, leads to change, inhibition and a variety of enzymatic activations, causing cellular damage and metabolic imbalance, resulting, for example, in apoptosis. 35 The antioxidant defense system can be either activated or inhibited in the presence of chemical stress; 36 activation or deactivation depends on the intensity and duration of the stress applied to the organism, as well as on the susceptibility of the specific organism to the stressing agent.…”

Section: Resultsmentioning

confidence: 99%

Effects of Copper and Cadmium Exposure on mRNA Expression of Catalase, Glutamine Synthetase, Cytochrome P450 and Heat Shock Protein 70 in Tambaqui Fish (Colossoma Macropomum)

Casanova¹,

Honda²,

Ferreira-Nozawa³

et al. 2013

GGG

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Abstract:In this work the authors evaluated the expression of cat, glu, cypP450, hsp70 and 18S RNA in Colossoma macropomum following exposure to copper and cadmium by semi-quantitative RT-PCR and quantitative qRT-PCR. In RT-PCR and qRT-PCR hsp70 expression was highest after exposure to copper for 3 hours (h). glu expression increased after exposure to cadmium, regardless of time. cat expression increased after the first hour of exposure to both metals, and decreased after 3 hs. cyp450 expression was higher when compared to controls in exposed fish regardless of metal and exposure time. cyp450 expression after exposure to copper for 3 h was twice that recorded after 1 h. In the presence of cadmium, cyp450 expression was higher in the animals exposed for 1 h and lower at 3 h. Therefore, toxicology tests and biomonitoring based on molecular markers should focus on a set of molecular and/or environmental parameters, rather than isolated markers.

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Section: Resultsmentioning

confidence: 99%

Effects of Copper and Cadmium Exposure on mRNA Expression of Catalase, Glutamine Synthetase, Cytochrome P450 and Heat Shock Protein 70 in Tambaqui Fish (Colossoma Macropomum)

Casanova¹,

Honda²,

Ferreira-Nozawa³

et al. 2013

GGG

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“…The RG levels increased from 0.20 μ mol/g (Control) to 0.36 μ mol/g in the pH 6 after fifteen days of exposure ( Figure 3f). Cossu et al [37] reports that under stress the antioxidant system of the animals may either be activated or inhibited. The activation and inhibition are two contradictory responses that are dependent on the duration and intensity of the stressors as well as on the sensitivity of the animals exposed.…”

Section: Reduced Glutathione Activitymentioning

confidence: 99%

Biomarker Response of Ocean Acidification Induced Hypercapnia on Marine Bivalve Donax cuneatus, Linnaeus 1758

Anand¹

2016

JAMB

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The present study reports the investigation of the sub lethal impacts of ocean acidification induced hypercapnia (pH 7.5 and 7.0) and leakage of sub sea bed Carbon Dioxide storage sites (pH 6.5-5.5) on marine bivalve Donax cuneatus, Linnaeus 1758 collected from the Gulf of Mannar province. Fifteen days experiment was carried out in laboratory microcosms in a closely monitored environment. The impacts of Hypercapnia on Biomarker enzymes indicating neurotoxicity (Acetylcholinesterase), oxidative stress (lipid peroxidation and catalase) and phase II biotransformation of xenobiotics (glutathione S transferase, and reduced glutathione) were estimated. The increase in lipid peroxidation and catalase activities suggest hypercapnia induced oxidative stress. The decreasing pH also increased the activity of reduced glutathione and glutathione S transferase which acts as detoxifying enzymes for Hypercapnia induced toxicity however the hypercapnic conditions inhibited the levels of acetylcholine esterase which indicates the polluted environment substantiating stress induced high rate of mortality at low pH levels. The above used biomarkers thus provide a valuable tool for monitoring ocean acidification induced hypercapnic stress at a sublethal level.

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“…Antioxidant defence systems neutralize chemical reactive compounds produced by endogenous pathways and/or xenobiotic metabolism (Cossu et al 2000, Viarengo et al 2007. When activated, defences can be insufficient to counteract ROS production and protect tissues from oxidative damage.…”

Section: Introductionmentioning

confidence: 99%

“…This effect is associated with oxidative disruption in the lipid components of cellular membranes, resulting in products of lipid degradation and free radicals, all extremely toxic for the cell , Viarengo et al 2007. Given the complex mixture of contaminants present in aquatic ecosystems, LPO constitutes a useful biomarker of damage since it reflects not only exposure to pollutants but also toxicity, and its response is nonspecific to any kind of contaminant (Cossu et al 2000;Viarengo et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Interspecific variability of endocrine disruption and oxidative stress in two bivalve species from the Ria Formosa Lagoon (south coast of Portugal)

Pereira¹,

Gomes²,

Cardoso³

et al. 2013

Sci. Mar.

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SUMMARY: Estuaries are highly productive ecosystems that have been increasingly affected by anthropogenic activities. In this study, the presence of endocrine disrupting compounds (EDCs) and their relation to oxidative stress were assessed in two bivalve species (mussels Mytilus galloprovincialis and clams Ruditapes decussatus) from the Ria Formosa Lagoon (south Portugal). We applied the alkali-labile phosphate (ALP) assay to determine vitellogenin-like protein levels, and the lipid peroxidation (LPO) method to assess oxidative damage. Mussels and clams exhibited the same pattern of ALP concentrations, with no differences between genders and higher levels in winter. R. decussatus showed higher ALP values than M. galloprovincialis, possibly associated with a stronger responsiveness of clams to EDCs or due to the influence of the reproductive cycle. Mussels exhibited a more evident seasonal effect for LPO, indicating that these organisms are more influenced by seasonal fluctuations in the water column compared to the sediment-associated clams. Both species appear to be under the influence of EDC exposure (which could induce Vg-like protein synthesis) and oxidative stressors (leading to lipid peroxidation) in the Ria Formosa Lagoon, particularly in winter, reflecting the presence of different contaminants in Ria Formosa and environmental changes, as well as complex interactions between them.Keywords: Ria Formosa Lagoon, Mytilus galloprovincialis, Ruditapes decussatus, endocrine disruption, vitellogenin-like proteins, lipid peroxidation. RESUMEN: Variabilidad interespecífica de disruptores endocrinos y estrés oxidatiVo en dos especies de biValVos de la ría formosa (costa sur de portugal). -Los estuarios son ecosistemas altamente productivos afectados por actividades antropogénicas. En este trabajo, la presencia de compuestos disruptores endocrinos (EDCs) y su relación con estrés oxidativo se ha evaluado en los bivalvos Mytilus galloprovincialis y Ruditapes decussatus en la laguna Ría Formosa (Portugal). Se utilizó el ensayo de fosfatos lábil alcalinos (ALP) para determinar los niveles de proteína tipo-vitelogenina y el método de peroxidación lipídica (LPO) para evaluar los daños oxidativos. Ambas especies exhibieron el mismo patrón de concentración de ALP: no hubo diferencias entre sexos pero si niveles más altos en invierno. R. decussatus presentó valores mayores de ALP que M. galloprovincialis posiblemente asociados a una capacidad de respuesta más elevada a los EDCs o al estado del ciclo reproductor. Los mejillones, al no estar asociados al sedimento como las almejas, mostraron variaciones estacionales en las concentraciones de LPO, indicando mayor sensibilidad a fluctuaciones ambientales en la columna de agua. Ambas especies parecen estar influenciadas (principalmente en invierno) por EDCs (posiblemente induciendo la síntesis de proteína tipo-vitelogenina) y factores de estrés oxidativo (activando la peroxidación lipídica); lo que refleja la presencia de diferentes contaminantes en la Ría Formosa y los cambios a...

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Antioxidant Biomarkers in Freshwater Bivalves, Unio tumidus, in Response to Different Contamination Profiles of Aquatic Sediments

Cited by 213 publications

References 55 publications

Effects of Copper and Cadmium Exposure on mRNA Expression of Catalase, Glutamine Synthetase, Cytochrome P450 and Heat Shock Protein 70 in Tambaqui Fish (Colossoma Macropomum)

Effects of Copper and Cadmium Exposure on mRNA Expression of Catalase, Glutamine Synthetase, Cytochrome P450 and Heat Shock Protein 70 in Tambaqui Fish (Colossoma Macropomum)

Biomarker Response of Ocean Acidification Induced Hypercapnia on Marine Bivalve Donax cuneatus, Linnaeus 1758

Interspecific variability of endocrine disruption and oxidative stress in two bivalve species from the Ria Formosa Lagoon (south coast of Portugal)

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