Antioxidant, Antimicrobial and Urease Inhibitory Activities of Phenolics-Rich Pomegranate Peel Hydro-Alcoholic Extracts

Abstract

This study was aimed at exploring biological properties of PoP for their potential use as an ingredient of choice in nutraceuticals and ethnic drug formulations. Pomegranate peel extracts (PoPx), derived from hydro-alcoholic extracts were evaluated for antioxidant, antimicrobial and potential antiulcer properties. Higher levels of total phenolics (427.19 mgGAE/g of extracts) recovered from hydro-alcoholic extracts exhibited significant (P < 0.05) ferric reducing antioxidant power (FRAP) and 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radicals scavenging activity. PoPx inhibited urease activity by 97.9% and IC50 for all extracts was estimated to be in a range of 30–44.4 μM. Potent inhibitory effects of PoP extracts were observed against Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Salmonella typhimurium and Aspergillus niger and minimum inhibitory concentration (MIC) was recorded between 0.25–0.89 mg/mL. The study indicates that PoPx of the white rind cultivar is a potential source of active biomolecules that could further be explored as a source of bioactive compounds and therapeutic agent against various ailments.\ud Practical Applications\ud \ud The findings of this study propose PoP of white rind cultivar as valuable waste of pomegranate juice processing industry. The proposed methodology for PoPx recovery and the bioactivities reported could equally benefit the processors for low cost recovery of protective biomolecules. The study further defines admissible levels of extracts being exploited by the industry for manufacturing nutraceuticals, functional food preparations and designing ethnopharmacological drugs of choice

Antimicrobial activity of pomegranate peel extracts performed by high pressure and enzymatic assisted extraction

“…Our results are in line with the results obtained by Dahham, Ali, Tabassum, and Khan (2010), who studied antimicrobial effect of pomegranate peel extracts against B. cereus, E. coli, S. aureus and P. aeruginosa considering their inhibition halos. Ismail et al (2016) also obtained inhibitory effects of pomegranate peel extracts against B. subtilis, S. aureus, P. aeruginosa, E.coli, S. typhimurium and A. niger. However, in our study the extraction methodologies used had no significant impact on the inhibition halos increase.…”

Bactericidal, protistocidal, nematodicidal properties and chemical composition of ethanol extract of Punica granatum peel

“…In vitro, methanolic extract of pomegranate peel caused inhibitory effect to the linear growth of six economically important fungal phytopathogens isolated from different hosts including: Botrytis cinerea, Colletotrichum dematium, Fusarium oxysporum, F. solani, Phoma spp., and Rhizoctonia solani (Mohamad et al, 2015). Potent inhibitory effects of PoP extracts were observed against Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Salmonella typhimurium and Aspergillus niger and minimum inhibitory concentration (MIC) was recorded between 0.25-0.89 mg/mL (Ismail et al, 2016).…”

Oxidative and Microbial Stability of Pomegranate Peel Extracts and Bagasse Supplemented Cookies

“…Incorporation of PoP and its extracts in various food preparations as natural preservatives and nutraceuticals have been widely reported in the literature. In addition to their ability to act as a natural solution to oxidative damages in fat based food systems, PoPx inhibits microbiological pathogenesis and improves nutraceutical profile of the finished goods (Naveena et al 2008;Kanatt et al 2010;Akhtar et al 2015;Ismail et al 2016).…”