Antioxidant, antimicrobial and neutrophil-modulating activities of herb extracts.

Denev, Petko; Kratchanova, Maria; Čı́ž, Milan; Lojek, Antonı́n; Vašíček, Ondřej; Blazheva, Denica; Nedelcheva, P.; Vojtek, Libor; Hyršl, Pavel

doi:10.18388/abp.2014_1907

Cited by 37 publications

(26 citation statements)

References 34 publications

(19 reference statements)

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“…Plants and herbs have been applied in both prevention and treatment of human disorders since ancient times [3][4][5]. Indeed, many investigations on herbs and plants have been conducted, and their efficacy proved as a compelling source of antioxidant, antimicrobial, anti-inflammatory [6], anticancer and neuroprotective agents [7,8].…”

Section: Introductionmentioning

confidence: 99%

Cucurbita Plants: From Farm to Industry

et al. 2019

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The Cucurbita genus, a member of Cucurbitaceae family, also known as cucurbits, is native to the Americas. Genus members, like Cucurbita pepo and Cucurbita maxima, have been used for centuries in folk medicine for treating gastrointestinal diseases and intestinal parasites. These pharmacological effects are mainly attributed to their phytochemical composition. Indeed, Cucurbita species are a natural source of carotenoids, tocopherols, phenols, terpenoids, saponins, sterols, fatty acids, functional carbohydrates, and polysaccharides, that beyond exerting remarkable biological effects, have also been increasingly exploited for biotechnological applications. In this article, we specifically cover the habitat, cultivation, phytochemical composition, and food preservative abilities of Cucurbita plants.

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Section: Introductionmentioning

confidence: 99%

Cucurbita Plants: From Farm to Industry

et al. 2019

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“…This is in accordance with our findings that ethanolic extract has stronger antimicrobial activity. Antibacterial activity of aronia acetonic extract were demonstrated against 11 foodborne pathogens [8,9]. It showed weak to medium strong activity for majority of tested strains.…”

Section: Discussionmentioning

confidence: 99%

“…Elliot and red chokeberry, Aronia arbutifolia [L.] Elliot. It contains high concentration of antioxidants (tannins, biphenols, flavonoids, anthocyanins, catechins), scientifically proven to prevent various disease [6,8,31]. Besides its antioxidant, antiinflammatory, anticancer, lipid-lowering, gastro-and hepatoprotective, antiaggregatory, antihypertensive and antidiabetic activity [12,16], this plant also showed antimicrobial effects in vitro against some bacteria [17] and against influenzae A virus [24].…”

Section: Introductionmentioning

confidence: 99%

Activity of Serbian Aronia prunifolia against Prototheca wickerhamii and Prototheca zopfii

Suvajdžić

Krstić²,

Stojanović³

et al. 2017

Acta Scientiae. Vet.

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Background: Beneficial effects of berries have been known from 16th century, but their antimicrobial effects have been explained scientifically only recently. The two most common aronia species are black chokeberry, Aronia melanocarpa [Michx.] Elliot and red chokeberry, Aronia arbutifolia [L.] Elliot. Purple chokeberry (Aronia prunifolia) is a hybrid of these two species. Protothecosis is a disease caused by achlorophyllous algae Prototheca species. Infections with Prototheca species are more common in veterinary medicine. The purpose of this study was to investigate the activity of Aronia prunifolia berries against Prototheca zopfii (P. zopfii) and Prototheca wickerhamii (P. wickerhamii).Materials, Methods & Results: Purple chokeberry juice was made by squeezing the fruits and evaporated to dryness. Extracts of purple aronia were obtained by maceration with ethanol 80 % (v/v) for 24 h. Prototheca zopfii was obtained from udder of cow with mastitis and Prototheca wickerhamii was isolated from human oral cavity. Total phenolic and flavonoid contents were analized using spectrophotometric methods. The chemical composition of the tested substances was determined by a high performance liquid chromatography (HPLC) method. The examination was conducted by a micro-dilution method according to the guidelines of Clinical and Laboratory Standards Institute (CLSI). Microsoft Excel program 2007 was used for statistical analysis. The antialgal activity was expressed as minimal inhibitory concentrations MIC99, MIC90 and MIC80, the lowest concentration which kills 99%, 90% and 80% of organisms, respectively. Furthermore, minimal algaecide concentration (MAC), the lowest concentration which kills 99.9% of organisms is determined, as well as break point, the lowest concentration at which there is no algal growth.Discussion: The content of ascorbic acid was twice as high in the ethanolic extract as in the juice. Content of polyphenolic compounds was high in both juice and ethanolic extract. The quantity of phenocarbonic acids in juice and ethanolic extract was relatively low. Some of them were found only in juice (ellagic, coumaric and gentisic acids) as opposed to others found only in ethanolic extract (chlorogenic acid). Flavonoids were also detected in juice and ethanolic extract. Extract was much richer in flavonoid content when compared to aronia juice. Catechin was present in concentration of 186.3 mg/ 100 g of dry matter in the aronia juice, and 680.65 mg/ 100 g of dry matter in the ethanolic extract, which was more than 3.6 times higher. Quercetin was found only in the extract. The rutin content was 12 times and the chrysin content was 2.5 times higher in the aronia extract. The biggest difference could be noted in the quantitative contents of anthocyanins, 26 times higher concentration in extract than in juice. In general, higher content of bioactive compounds could be observed in the extract than in the juice. The results showed that the ethanolic extract of aronia fruits exhibited antialgal activity against both Prototheca species, while the juice showed no antialgal activity. This difference in antialgal effect is presumably related to the high content of several groups of biocompounds, especially catechin and anthocyanins, present in the ethanolic extract, and probably their synergistic action. There is no comparable data of antialgal effects of aronia in literature.

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“…The CL of human whole blood and isolated neutrophils was evaluated in a 96-well plate luminometer Orion II (Berthold Detection Systems GmbH, Germany). The principle of the method has been previously described (Denev et al ., 2014 ). Briefly, each reaction mixture consisted of 100× diluted whole blood in HBSS, 1 mM luminol, and berberine in various concentrations between 10 and 500 μM.…”

Section: Methodsmentioning

confidence: 99%

The effects of berberine on reactive oxygen species production in human neutrophils and in cell-free assays

Kassab

Vašíček

Čı́ž

et al. 2017

Interdisciplinary Toxicology

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The health benefits of berberine have been recognized for years. Even so, its effects on human neutrophils, the first line of immune defense, have not been reported. The purpose of this study was to investigate the effects of berberine on the human neutrophil oxidative burst. Reactive oxygen species production was analyzed by luminol-enhanced chemiluminescence. The analysis was performed in spontaneous and stimulated (phorbol myristate acetate (PMA) or opsonized zymosan particles (OZP)) whole blood and isolated neutrophils in the presence or absence of berberine. The effects of berberine on oxidant production in cell-free assays were evaluated using luminescence (H2O2-peroxidase-luminol) and fluorescence (Oxygen Radical Absorbance Capacity – ORAC) techniques. Berberine decreased the production of reactive oxygen species in human whole blood and isolated neutrophils stimulated with either PMA or OZP with a different efficiency (EC50 was 69 μM and 197 μM for PMA and OZP, respectively). The effect was more pronounced in isolated neutrophils. Cell-free assays showed the antioxidant activity of berberine against peroxyl radicals and hydrogen peroxide. Based on our results, we suggest that the effects of berberine on reactive oxygen species production in human neutrophils are due to its antioxidant activity.

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Antioxidant, antimicrobial and neutrophil-modulating activities of herb extracts.

Cited by 37 publications

References 34 publications

Cucurbita Plants: From Farm to Industry

Cucurbita Plants: From Farm to Industry

Activity of Serbian Aronia prunifolia against Prototheca wickerhamii and Prototheca zopfii

The effects of berberine on reactive oxygen species production in human neutrophils and in cell-free assays

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