Antioxidant, antimicrobial and cytotoxic potential of silver nanoparticles synthesized using flavonoid rich alcoholic leaves extract of Reinwardtia indica

Abstract

The present work discusses the establishment of a green route for the rapid synthesis of silver nanoparticles (AgNPs) using an alcoholic extract of Reinwardtia indica (AERI) leaves which act as a reducing as well as a capping agent. The change in color from yellowish green to dark brown confirmed the synthesis of AgNPs. A characteristic surface plasmon resonance (SPR) band at 436 nm advocated the presence of AgNPs. The synthesis process was optimized using one factor at a time approach where 1.0 mM AgNO concentration, 5 mL 0.4% (v/v) of AER inoculum dose and 30 min of sunlight exposure were found to be the optimum conditions. The synthesized AgNPs was characterized by several characterizing techniques such as HR- TEM, SAED, HR-SEM, EDX, XRD, FTIR and AFM analysis. For evaluation and comparison of AgNPs with AERI used human pathogen E. coli, P. aeurogenosa, S. aeurus and C. albicans for antimicrobial, for cytotoxicity study SiHa cell line at concentration of (10, 50, 100, 250 and 500 µg mL) and for enzymatic assay superoxide dismutase, catalase, malondialdehyde and glutathione peroxidase method were used. The size of nanoparticle in the range of 3-15 nm was confirmed TEM, spherical shape by SEM and crystal lattice nature by XRD. AFM results revealed the 2 D and 3 D pattern of particle scatter nature on the surface. This protocol as simple, rapid, one step, eco-friendly, nontoxic and AgNPs showed strong antimicrobial activity as well as cytotoxic potential in comparison to AERI.

[Retracted] Silver Nanoparticles Stabilized by Poly (Vinyl Pyrrolidone) with Potential Anticancer Activity towards Prostate Cancer

“…It could also be observed from the spectra of AgNPs-PVP (trace C) that the absence of the peaks of AgNO 3 and the concomitant presence of the peaks of PVP confirmed the reduction and coating of silver with PVP and the formation of AgNPs-coated polymers. A similar result was also observed by other researchers, who concluded the absent peaks of AgNO 3 in AgNPs confirmed the reduction of silver ions due to the reduction of capping material [52].…”

Multitherapeutic Efficacy of Curly Kale Extract Fabricated Biogenic Silver Nanoparticles

“…This result is analogous to the previous AgNPs synthesis result. 58 This outcome of the present investigation proposes that phytochemicals and secondary metabolites existing in plant food leafy vegetables in BroL extract works, like reducing agent and capping agents, are derived from leafy vegetables that are eco-friendly and non-toxic. Among the natural resource, plant leaf extracts are easily obtainable, harmless and generally non-toxic and it possesses numerous bioactive compounds which help in the Ag+ (ions) reduction process.…”

Silver-Loaded Carbon and Phosphorous Co-Doped Boron Nitride Quantum Dots (Ag@CP-BNQDs) for Efficient Organic Waste Removal: Theoretical and Experimental Investigations

“…The signals at 552 cm –1 could be detected for BNQDs and symmetric stretching vibration mode of P–O–P –. Another peak was observed at 1010 cm –1 due to the B–N–O vibration mode and it is also similar to the related results of BNQDs elsewhere, it may also be due to the P–O–Ag vibration mode., – Moreover, the signal at 1384 cm –1 resulted from the stretching vibration of C–O or due to the bending mode of B–OH and stretching mode of B–N., In addition, the peak at 1659 cm –1 resulted from the vibration mode of the Ag–N bond Another peak was observed at 3242 cm –1 due to the Ag–N vibration mode as well The two peaks between 1300 and 1700 cm –1 are assigned for the heterocyclic C–N stretching vibration mode as well …”