Antioxidant, antimicrobial and antiverotoxic potentials of extracts of Curtisia dentata

Doughari, James Hamuel; Ndakidemi, Patrick A.; Human, Izanne Susan; Benadé, AJ Spinnler

doi:10.1016/j.jep.2012.03.051

Cited by 69 publications

(45 citation statements)

References 19 publications

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“…Total saponins content extracted from all the three spice extracts was found to be lowest in polar solvents. Our results are contrary to the reports in which the total saponins contents were found in greater quantity in polar solvent extracts of different plants 33,34,35 .…”

Section: Resultscontrasting

confidence: 99%

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2017

IJPSR

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ABSTRACT:Spices have been used since long to enhance the flavor and aroma of our foods. Besides, they also produce several medicinal effects and are used in treating various clinical ailments. The different phytochemicals present greatly influence the biological activities possessed by plants/spices. The present research work investigates the free radical scavenging and acetylcholinesterase (AchE) inhibition activity of different extracts of Elletaria cardamomum, Piper nigrum and Syzygium aromaticum. Total phenol, flavonoid, condensed tannins and saponin contents were also measured. Among the different extracts of spices evaluated for DPPH free radical scavenging, ethanolic extract of S. aromaticum exhibited the highest inhibition with IC 50 value of 42±7.4 µg/ml. This high radical scavenging activity can be directly correlated with the presence of high total phenolic content (310±6.87 mg GAEs/g extract) possessed by the extract. The ethanolic extracts of all three spices had shown better inhibition activity against AChE than other extracts. At 500 µg/mL concentration, S. aromaticum and E. cardamomum ethanolic extracts showed 52.22±3.7 % and 52.95±2.9 % AchE inhibition respectively. These findings suggest that these spices could act as an anticholinesterase agent and also are an efficient free radical scavenger, which may be helpful in preventing or alleviating patients suffering from Alzheimer's disease.

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Section: Resultscontrasting

confidence: 99%

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2017

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“…Verotoxin or shiga toxin-producing bacterial strains are associated with a broad spectrum of human illnesses throughout the world, ranging from mild diarrhea to haemorrhagic colitis (HC), HUS and thrombotic thrombocytopenic purpura (TTP). Complications arising from antibiotic treatment of verotoxic related human infections have also been reported (Doughari et al, 2010). Though the wastewater samples investigated are not directly consumed, the presence of a significant proportion (47%) of verotoxin producing bacteria from the various water samples investigated is a cause for concern since the samples often gets discharged into large water bodies or are utilized for irrigation purposes.…”

Section: Discussionmentioning

confidence: 99%

Virulence factors and antibiotic susceptibility among verotoxic non O157: H7 Escherichia coli isolates obtained from water and wastewater samples in Cape Town, South Africa.

Doughari¹,

Ndakidemi²,

Human³

et al. 2011

Afr. J. Biotechnol.

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Forty eight samples (30 wastewater and 18 river water) were collected between July and November, 2010, from different sources in Cape Town, South Africa in order to characterize verotoxic non O157: H7 Escherichia coli strains. Samples (1 ml) were inoculated into MacConkey broth (MB, 9 ml) and incubated at 37°C for 24 h, after which a loopful of the MB was then spread onto Eosin Methylene Blue (EMB) and further incubated for 24 h at 37°C in order to isolate E. coli. The identification of isolates was done using standard biochemical procedures; and confirmed serologically using E. coli polyvalent antisera (Bioweb, SA). Isolates were also characterized for virulence factors such as verotoxin, haemolysin, gelatinase, extended spectrum beta lactamases (ESBLs), cell surface hydrophobicity and bacterial serum resistance, as well as susceptibility (using disc diffusion method) to stem bark extracts of Curtisia dentata. Results showed the presence of different serotypes of E. coli (69 isolates in all) including 026: H11, 055, O111: NM, O126, O44, O124, O96:H9, O103:H2, O145: NM and O145:H2. Over 60% of the isolates exhibited serum resistance, haemolysin and gelatinase production, 81% exhibited cell surface hydrophobicity and over 52% produced ESBLs. Results also showed that while 60% of the isolates showed various levels of resistance to different antibiotics [ampicillin (10 µg), cefuroxime, cephalexin, ceftazidime and tetracycline (30 µg in each case) (multidrug resistance index (MDRI) values 4.20 to 5.60%)], only 28% were resistant to ethanol stem bark extracts of C. dentata (MIC, 70 to 150 mg/ml). The presence of pathogenic verotoxic antibiotic resistant E. coli in these water sources is a threat to water quality and food security and C. dentata has a potential for sourcing novel antibiotic substances for chemotherapy against these resistant pathogenic strains of E. coli.

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“…Presently, much attention has been given to plant-derived essential oils, as they possess various pharmaceutical properties such as antibacterial, antioxidant, antiviral, anti-insecticidal, anti-mycotic, and anti-toxinogenic activities [27][28][29][30]. From the ancient times, many plant oils have been used as topical antiseptics and also it is used to improve food safety and quality [16].…”

Section: Discussionmentioning

confidence: 99%

Antibacterial and Antioxidant Activities of Stem Bark Essential Oil Constituents of Litsea Glutinosa C. B. Rob.

Arunodaya

Krishna

Shashikumar

et al. 2016

Int J Pharm Pharm Sci

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Objective: To evaluate the chemical composition, antibacterial and antioxidant properties of stem bark essential oil of Litsea glutinosa C. B. Rob. Methods:The essential oil isolated from stem bark of L. glutinosa and their chemical composition was analyzed by gas chromatography coupled with mass spectrometry detector. The in vitro antibacterial activity of the stem bark essential oil was investigated against eight human pathogenic bacterial clinical isolates using agar disc diffusion method and MIC value was determined by modified resazurin microtitre-plate assay. The antioxidant activity of essential oil was measured by 2, 2-diphenyl-1-picrylhydrazyl radical (DPPH), 2, 2-azinobis-3-ethylbenzothiazoline-6-sulphonate radical cation (ABTS) and β-carotene bleaching assay.Results: GC-MS analysis of stem bark essential oil resulted in the identification of 37 compounds, off which 9,12-octadecadienoic acid (62.57%), hexadecanoic acid (12.68%), stigmast-5-en-3-ol (6.87%) and vitamin E (2.51%) were the main constituents representing 84.63% of the oil. The determination of in vitro antibacterial activity of stem bark essential oil resulted in significant inhibition zone (15.00±0.57 mm) and MIC value (0.15±0.15×10 -2 Conclusion: L. glutinosa stem bark essential oil showed potential antibacterial activity against the Vibrio cholera. The results of this investigation supported the ethnomedical claim of essential oil as a demulcent, antidiarrheal and antioxidant drug. mg/ml) against the pathogenic bacteria Vibrio cholera followed by Pseudomonas aeruginosa and Salmonella typhi. The results of DPPH radical scavenging (IC50:4.540±0.06 µg/ml), ABTS (IC50:256.02±0.06 µg/ml) and β-carotene bleaching assay (%I: 78.51±0.42 %) showed significant in vitro antioxidant property.

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Antioxidant, antimicrobial and antiverotoxic potentials of extracts of Curtisia dentata

Cited by 69 publications

References 19 publications

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Virulence factors and antibiotic susceptibility among verotoxic non O157: H7 Escherichia coli isolates obtained from water and wastewater samples in Cape Town, South Africa.

Antibacterial and Antioxidant Activities of Stem Bark Essential Oil Constituents of Litsea Glutinosa C. B. Rob.

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