Antioxidant, antimicrobial activity and in silico PASS prediction of Annona reticulata Linn. root extract

Jamkhande, Prasad G.; Wattamwar, Amruta S.; Pekamwar, Sanjay S.; Chandak, Prakash G.

doi:10.1016/j.bjbas.2014.05.008

Cited by 27 publications

(19 citation statements)

References 46 publications

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“…The extracts that had antibacterial activity were also examined for antioxidant activity using the DPPH method; DPPH is a relatively stable free radical readily reducible by antioxidants. The DPPH method is routinely used as a valid, accurate and sensitive procedure to evaluate the radical scavenging activity of antioxidants [ 35 ]. All the extracts examined displayed significantly ( p < 0.05) higher radical scavenging activity (EC 50 0.007–0.02 mg/mL) than L-ascorbic acid (EC 50 0.037 mg/mL); the value for L-ascorbic acid in the present study compared well with a value that has been reported in a previous study (0.043 mg/mL) [ 19 ].…”

Section: Discussionmentioning

confidence: 99%

Terminalia bellirica fruit extracts: in-vitro antibacterial activity against selected multidrug-resistant bacteria, radical scavenging activity and cytotoxicity study on BHK-21 cells

Dharmaratne

Manoraj

Thevanesam

et al. 2018

BMC Complement Altern Med

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BackgroundIdentification of novel sources for developing new antibiotics is imperative with the emergence of antibiotic resistant bacteria. The fruits of Terminalia bellirica (Gaertn) Roxb., widely used in traditional medicine, were evaluated for antibacterial activity against multidrug-resistant (MDR) bacteria, antioxidant activity and cytotoxicity.MethodsTwelve solvent extracts of T. bellirica fruits were prepared by direct aqueous extraction and sequential extraction with dichloromethane, methanol and water using Soxhlet, bottle-shaker and ultrasound sonicator methods. Antibacterial activity of the extracts was tested against 16 strains MDR bacteria—methicillin-resistant Staphylococcus aureus (MRSA), extended spectrum β-lactamase (ESBL) producing Escherichia coli and MDR Acinetobacter spp., Klebsiella pneumoniae and Pseudomonas aeruginosa—and 4 control organisms, using the cut-well diffusion method. The minimum inhibitory concentration (MIC) was determined using an agar dilution method. The radical scavenging activity of six antibacterial extracts was screened against 2,2′-diphenyl-2-picrylhydrazyl (DPPH) and correlation was established between EC50 (50% effective concentration) values and the total phenolic content (TPC). Cytotoxicity was determined for the most potent antibacterial extract on baby hamster kidney (BHK-21) cells by Tryphan Blue exclusion method. Statistical analysis was carried out by one-way analysis of variance at significant level p < 0.05 using “SigmaPlot 10” and “R 3.2.0” software.ResultsAll aqueous and methanol extracts displayed antibacterial activity (MIC 0.25–4 mg/mL) against all strains of MRSA, MDR Acinetobacter spp. and MDR P. aeruginosa. The sequential aqueous extracts (MIC, 4 mg/mL) inhibited ESBL producing-E. coli. None of the extracts exhibited activity against MDR K. pneumoniae (MIC > 5 mg/mL). The sequential methanol extract (Soxhlet) recorded high antibacterial activity and the highest DPPH radical scavenging activity (EC50, 6.99 ± 0.15 ppm) and TPC content (188.71 ± 2.12 GAE mg/g).The IC50 (50% inhibition concentration) values of the most potent antibacterial extract—the direct aqueous extract from reflux method—on BHK-21 cells were 2.62 ± 0.06 and 1.45 ± 0.08 mg/ml with 24 and 48 h exposure, respectively.ConclusionsResults indicate that T. bellirica fruit is a potential source for developing broad-spectrum antibacterial drugs against MDR bacteria, which are non-toxic to mammalian cells and impart health benefits by high antioxidant activity.

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Section: Discussionmentioning

confidence: 99%

Terminalia bellirica fruit extracts: in-vitro antibacterial activity against selected multidrug-resistant bacteria, radical scavenging activity and cytotoxicity study on BHK-21 cells

Dharmaratne

Manoraj

Thevanesam

et al. 2018

BMC Complement Altern Med

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“…Therefore, we chose to study the anti-inflammatory properties using a diabetic rat model to relate the pharmacological significance with the ethnobotanical claims by locals. Further, studies carried out using extracts of different parts of the plant have been reported to have anti-cancer (23), anti-inflammatory (24), anti-oxidant (25), hypoglycemic (26), analgesic (27), and anti-ulcerative effects (28), as well as wound healing activity (29).…”

Section: Introductionmentioning

confidence: 99%

Antidiabetic, Antihyperlipidemic, Antioxidant, Anti-inflammatory Activities of Ethanolic Seed Extract of Annona reticulata L. in Streptozotocin Induced Diabetic Rats

Wen

Lin²,

Ti³

2019

Front. Endocrinol.

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Annona reticulata L. (Bullock's heart) is a pantropic tree commonly known as custard apple, which is used therapeutically for a variety of maladies. The present research was carried out to evaluate the possible protective effects of Annona reticulata L. (A. reticulata) ethanolic seed extract on an experimentally induced type 2 diabetes rat model. Male Albino Wistar rats were randomly divided into five groups with six animals in each group viz., control rats in group I, diabetic rats in group II, diabetic rats with 50 and 100 mg/kg/bw of ethanolic seed extract of A. reticulata in groups III and IV, respectively, and diabetic rats with metformin in group V. Treatment was given for 42 consecutive days through oral route by oro-gastric gavage. Administration of A. reticulata seed extract to diabetes rats significantly restored the alterations in the levels of body weight, food and water intake, fasting blood glucose (FBG), insulin levels, insulin sensitivity, HbA1c, HOMA-IR, islet area and insulin positive cells. Furthermore, A. reticulata significantly decreased the levels of triglycerides, cholesterol, LDL, and significantly increased the HDL in diabetic rats. A. reticulata effectively ameliorated the enzymatic (ALT, AST, ALP, GGT) and modification of histopathological changes in diabetic rats. The serum levels of the BUN, creatinine levels, uric acid, urine volume, and urinary protein were significantly declined with a significant elevation in CCr in diabetic rats treated with A. reticulata. MDA and NO levels were significantly reduced with an enhancement in SOD, CAT, and GPx antioxidant enzyme activities in the kidney, liver, and pancreas of diabetic rats treated with A. reticulata. Diabetic rats treated with A. reticulata have shown up-regulation in mRNA expression levels of nuclear factor erythroid 2-related factor 2 (Nrf2), NAD(P)H:quinone oxidoreductase 1 (NQO1), Heme oxygenase-1 (HO-1) and protein expression level of Nrf2 with diminution in Keap1 mRNA expression level in pancreas, kidney, and liver. From the outcome of the current results, it can be inferred that seed extract of A. reticulata exhibits a protective effect in diabetic rats through its anti-diabetic, anti-hyperlipidemic, antioxidant and anti-inflammatory effects and could be considered as a promising treatment therapy in the treatment of diabetes mellitus.

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“…Author(s) agree that this article remains permanently open access under the terms of the Creative Commons Attribution License 4.0 International License Loaeza et al, 2011), antidiabetic (Florence et al, 2014), anticancer (Wang et al, 2014), antiacetylcholinesterase (Tsai and Lee, 2010), antimicrobial (Jamkhande et al, 2014), among others. Activity has been reported for various parts of the plant such as roots (Jamkhande et al, 2014), stem bark , leaves (Matsumoto et al, 2014), fruit pulp (Clerici and CarvalhoSilva, 2011) and seeds (Ribeiro et al, 2014).…”

Section: Introductionmentioning

confidence: 97%

“…Activity has been reported for various parts of the plant such as roots (Jamkhande et al, 2014), stem bark , leaves (Matsumoto et al, 2014), fruit pulp (Clerici and CarvalhoSilva, 2011) and seeds (Ribeiro et al, 2014). A huge diversity of substances associated to a range of medicinal and nutritional (Pareek et al, 2011) benefits has also been described.…”

Section: Introductionmentioning

confidence: 99%

Fatty acid profile and bioactivity from Annona hypoglauca seeds oil

Santos¹,

Filho²,

Chagas³

et al. 2015

Afr. J. Biotechnol.

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Plants from Annona (Annonaceae) genus are present in tropical regions, where they have economic and medicinal potential. Information on the fatty acids profile and bioactivity from seed oil of Annona species are incipient. The objective of this work was to investigate Annona hypoglauca seeds oil in terms of its yield, composition and biological activity (acetylcholinesterase enzyme inhibition, bactericidal and fungicidal activity). Fatty acids profiles were determined by Gas Chromatography equipped with Flame Ionization Detector. Oil yield reached about 15% and the major constituents detected were ω-9 oleic acid (42.65%) and ω-6 linoleic acid (29.63%). A. hypoglauca oil was potent for acetylcholinesterase inhibition (79.55%), and presented high and selective bioactivity against Candida albicans.

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Antioxidant, antimicrobial activity and in silico PASS prediction of Annona reticulata Linn. root extract

Cited by 27 publications

References 46 publications

Terminalia bellirica fruit extracts: in-vitro antibacterial activity against selected multidrug-resistant bacteria, radical scavenging activity and cytotoxicity study on BHK-21 cells

Terminalia bellirica fruit extracts: in-vitro antibacterial activity against selected multidrug-resistant bacteria, radical scavenging activity and cytotoxicity study on BHK-21 cells

Antidiabetic, Antihyperlipidemic, Antioxidant, Anti-inflammatory Activities of Ethanolic Seed Extract of Annona reticulata L. in Streptozotocin Induced Diabetic Rats

Fatty acid profile and bioactivity from Annona hypoglauca seeds oil

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