Antioxidant, antimicrobial activity and bioactive compounds of Bergenia ciliata Sternb.: A valuable medicinal herb of Sikkim Himalaya

Abstract

Bergenia ciliata Sternb., commonly known as Paashaanbhed, is a well known herb of Sikkim Himalaya with various pharmaceutical properties. However, scientific exploration of B. ciliata, growing in the Sikkim Himalaya, for phytochemicals and pharmacological properties is in infancy. With this view, the present study was undertaken to investigate B. ciliata leaf extracts for antioxidant, antimicrobial activity and bioactive compounds. Three solvents viz., methanol, ethyl acetate and hexane were used for extraction and the respective leaf extracts were analyzed for total phenolic and flavonoid contents along with the antioxidant and antimicrobial activities. Amongst the tested solvents, methanol was found to be the best solvent for extraction with highest total phenolic contents and the lowest IC50 values for the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays. Methanol extract also exhibited effective antimicrobial activity, particularly against bacteria and actinomycetes. Further, high performance liquid chromatography (HPLC) analysis revealed that methanolic extract contains the highest amount of all the three analyzed bioactive compounds viz. bergenin, catechin and gallic acid. The current study suggests that the methanol extract of B. ciliata is a potential source of natural antioxidant and antimicrobial compounds that can be used in food and drug industries.

Effective anti-cancer property of Pouteria sapota leaf on breast cancer cell lines

“…The radical scavenging activity of P.sopata leaf extract was estimated using DPPH method [7] , [8] , [9] . In this assay, 0.1 mM solution of DPPH in methanol was prepared and 1 ml of this solution was added to 3 ml of the aqueous extracts of the sample at different concentrations (25, 50, 75 and 100 µg/ml) with the standard ascorbic acid.…”

Bergenia ciliata as antibacterial agent

“…Boiled roots of Bergenia ciliata in combination with salt have been reported to be used for the cure of asthma. Its use for the treatment and prevention of piles and cancer is also reported [7,19,20].…”

“…The methanol, ethyl acetate and hexane extracts of Bergenia ciliata were evaluated against the selected bacterial strains including Nocardia tenerifensis, Bacillus megaterium and Bacillus subtilis. Among all the extracts the methanolic extract was found to have better antibacterial potential [20]. The antibacterial potential of the crude extract of Bergenia ciliata was determined against six bacterial strains including Bordetella bronchiseptica, Salmonella Setubal, Escherichia coli, Micrococcus luteus, Staphylococcus aureus and Salmonella typhimurium.…”

Major Bioactive Alkaloids and Biological Activities of Tabernaemontana Species (Apocynaceae)

“…Approximately 25% of modern medication has been established on the core basis of plant-related compounds [ 177 ]. Thus, the active screening of medicinal plants is imperative for the discovery of new antibacterial compounds [ 178 ]. The indole alkaloids of Tabernaemontana exhibit a wide range of pharmacological activities, including antibacterial activity against Gram-positive and Gram-negative bacteria [ 133 ].…”