Antioxidant, Antihemolytic, Antihyperuricemic, Anti-inflammatory Activity of Algerian Germander Methanolic Extract

Krache, Imane; Boussoualim, Naouel; Trabsa, Hayat; Ouhida, Sorya; Baghiani, Abderrahmane; Arrar, Lekhmici

doi:10.9734/arrb/2018/38232

Cited by 5 publications

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“…Thus a fractionation of the hydroethanolic and ethanolic extracts of Codiaeum variegatum was carried out and the effect of each fraction was evaluated on a polyxenic culture of a clinical isolate of E. histolytica. Although the axenic culture of HM1:IMSS strain is mostly used for antiamoebic assays [17] [35], polyxenic culture of E. histolytica clinical isolate was previously used in an in vitro model for the evaluation of the amoebicidal activity of extracts and fraction [18] [26] [36]. Upon fractionation of the extracts, the antiamoebic activity decreased.…”

Section: Discussionmentioning

confidence: 99%

Hydro-Alcoholic Leaf Extract and Fractions of Codiaeum variegatum (var. Mollucanum) Exhibited an Improved Anti-Amoebic and Moderate Anti-Oxidant Potential

Nsangou¹,

Fondjou²,

Mandou³

et al. 2023

AJPS

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Amoebiasis, classified as the third intestinal parasitic infection, represents a public health problem in low-income countries where hygiene and sanitation conditions are poor. With the resurgence of resistant pathogenic strains as well as ancestral considerations in developing countries such as Cameroon, many people rely on medicinal plants to treat a plethora of diseases. This work aimed to highlight the anti-amoebic and anti-oxidant potential of Codiaeum variegatum extracts and fractions. The anti-amoebic potential of C. variegatum was assayed on the polyxenic culture of the clinical isolates of E. histolytica. Then, the anti-oxidant potential of the ethanolic/hydroethanolic extracts and fractions was evaluated through DPPH radical scavenging, iron reduction (FRAP), lipid peroxidation inhibitory potential and total antioxidant capacity tests followed by the determination of phenolic compound and flavonoid content. It was found that the fractionation process decreased the amoebicidal activities of C. variegatum leaf extracts. However hydroethanolic extract (CI 50 : 10.08 ± 0.42, 5.18 ± 0.09, 5.18 ± 0.09 µg/mL respectively after 24, 48 and 72 hours) was more active than ethanolic extract (CI 50 : 15.59 ± 6.17; 9.61 ± 2.37; 6.26 ± 3.22 µg/mL respectively after 24, 48 and 72 hours). Interestingly, the activities of hydroethanolic extract were significantly non-different compared to metronidazole CI 50 : 8.42 ± 0.44, 6.45 ± 0.22 and 3.42 ± 0.33 µg/mL, respectively after 24, 48 and 72 hours). Ethanolic extract and EF5 showed higher Phenolic compound contents and higher antioxidant activity How to cite this paper: Nsangou

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Section: Discussionmentioning

confidence: 99%

Hydro-Alcoholic Leaf Extract and Fractions of Codiaeum variegatum (var. Mollucanum) Exhibited an Improved Anti-Amoebic and Moderate Anti-Oxidant Potential

Nsangou¹,

Fondjou²,

Mandou³

et al. 2023

AJPS

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“…The search for the efficacy of plant extracts and the determination of their mechanism of action are major and permanent challenges for the valorization of phytotherapy [31]. In fact, Plants are the reservoir of important bioactive molecules classified as phenolics, alkaloids, carotenoids, organosulfur compounds, etc.…”

Section: Discussionmentioning

confidence: 99%

Codiaeum Variegatum Hydro Alcoholic Leaf Extracts and Their Fractions Inhibit Pro-Inflammatory Mediators in Vitro

Nsangou,

Mandou,

Fondjou

et al. 2023

JBM

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Codiaeum variegatum has been widely investigated for its biological proprieties ranging from the antiamoebic potential to the phytochemical analysis. The aim of the present study was to evaluate the anti-inflammatory potential of C. variegatum leaf extracts and fractions. A primary macrophage culture activated by Saccharomyces cereviseae (SC) was used to evaluate cell cytotoxicity and anti-inflammatory potential of the plant extracts and fractions. Macrophages were treated with different concentrations (0.1; 1; 10 and 100 μg/mL) of the extracts/fractions for the inhibition of 5-lipoxygenase activity, nitric oxide (NO) and Tumor Necrosis Factor Alpha (TNF-α) production. No significant difference was observed on cell viability in the presence of extracts and fractions at tested concentration during the incubation period. Extracts and fractions of C. variegatum inhibited the 5-lipoxygenase activity, NO and TNF-α production by viable primary mouse macrophages in a concentrationdependent manner. The fractionation process increased anti-inflammatory activity. Among fractions, HEF2, HEF3, HEF5, EEF1, EEF3 and EEF5 exhibited the best anti-inflammatory potential. C. variegatum extracts and fractions exhibited a greater anti-inflammatory potential throughout the inhibition of pro-inflammatory mediators such as NO, 5-Lox and TNF-α.

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“…The total flavonoid content of each extract was determined by a colorimetric method 13 . Each sample (1 ml) was mixed with 1ml of aluminum chloride (AlCl3) solution (2%) and allowed to stand for 15 min.…”

Section: Determination Of Total Flavonoid Contentsmentioning

confidence: 99%

The In vivo and in vitro antioxidant and anti-hemolytic effect of Algerian Centaurea calcitrapa L. extracts

Trabsa

Baghiani

Boussoualim

et al. 2020

J. Drug Delivery Ther.

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In the present study, aerial part of Centaurea calcitrapa L. were extracted with solvent of varying polarity allowed their separation into three main subfractions, the analysis of methanol crud (CrE), chloroform (ChE) and ethyl acetate (EaE) extracts, showed that the EaE contains the highest amount of flavonoids (50,71 ± 0,65 mg Eq / Quercetin g dry extract and 31,96 ± 0,39 mg Eq Rutin / g dry extract). Using DPPH assay, the highest activity was observed with EaE (IC50 = 0,037 ± 0,0006 mg / ml). The β-carotene / linoleic acid bleaching assay revealed that the extracts have a very important antioxidant activity. The results showed that CrE has the highest antioxidant activity. The antioxidant activity of the CrE is confirmed by an in vivo assay in mice, using two doses: CrD1 (50 mg/kg/day) and CrD2 (100 mg/kg/day) during 21 days. Total antioxidant capacity of plasma and red blood cells was measured. The half-life (HT50), which corresponds to 50% of cell lysis was calculated, the results showed that both groups treated with plant extract had a protective effect against erythrocytes hemolysis (CrD2: HT50= 167,3 ± 3,72 min). The CrD2 group showed a strong scavenging activity using DPPH assay (51,64 ± 5,24 %), higher than that of Vit C group (38,92 ± 1,72 %). All results confirmed that the extracts have a dose dependent effect on the growth of overall antioxidant defenses. These results support the use of this plant against anti-inflammatory diseases in traditional medicine. Keywords: Centaurea calcitrapa L., in vivo-antioxidant activity, DPPH, hemolysis, flavonoids.

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Antioxidant, Antihemolytic, Antihyperuricemic, Anti-inflammatory Activity of Algerian Germander Methanolic Extract

Cited by 5 publications

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Hydro-Alcoholic Leaf Extract and Fractions of <i>Codiaeum variegatum</i> (var. Mollucanum) Exhibited an Improved Anti-Amoebic and Moderate Anti-Oxidant Potential

Hydro-Alcoholic Leaf Extract and Fractions of <i>Codiaeum variegatum</i> (var. Mollucanum) Exhibited an Improved Anti-Amoebic and Moderate Anti-Oxidant Potential

Codiaeum Variegatum Hydro Alcoholic Leaf Extracts and Their Fractions Inhibit Pro-Inflammatory Mediators <i>in Vitro</i>

The In vivo and in vitro antioxidant and anti-hemolytic effect of Algerian Centaurea calcitrapa L. extracts

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Antioxidant, Antihemolytic, Antihyperuricemic, Anti-inflammatory Activity of Algerian Germander Methanolic Extract

Cited by 5 publications

References 0 publications

Hydro-Alcoholic Leaf Extract and Fractions of &lt;i&gt;Codiaeum variegatum&lt;/i&gt; (var. Mollucanum) Exhibited an Improved Anti-Amoebic and Moderate Anti-Oxidant Potential

Hydro-Alcoholic Leaf Extract and Fractions of &lt;i&gt;Codiaeum variegatum&lt;/i&gt; (var. Mollucanum) Exhibited an Improved Anti-Amoebic and Moderate Anti-Oxidant Potential

Codiaeum Variegatum Hydro Alcoholic Leaf Extracts and Their Fractions Inhibit Pro-Inflammatory Mediators &lt;i&gt;in Vitro&lt;/i&gt;

The In vivo and in vitro antioxidant and anti-hemolytic effect of Algerian Centaurea calcitrapa L. extracts

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Hydro-Alcoholic Leaf Extract and Fractions of <i>Codiaeum variegatum</i> (var. Mollucanum) Exhibited an Improved Anti-Amoebic and Moderate Anti-Oxidant Potential

Hydro-Alcoholic Leaf Extract and Fractions of <i>Codiaeum variegatum</i> (var. Mollucanum) Exhibited an Improved Anti-Amoebic and Moderate Anti-Oxidant Potential

Codiaeum Variegatum Hydro Alcoholic Leaf Extracts and Their Fractions Inhibit Pro-Inflammatory Mediators <i>in Vitro</i>