Antioxidant, Antidiabetic, and Anti-obesity Potential of Ipomoea reptans Poir Leaves

Abstract

Ipomoea reptans Poir or kangkung is a popular leafy vegetable, a favorite to people in Asian countries. However, limited information is available on their bioactivities. In the present study, the antioxidant, antidiabetic, and anti-obesity potential of I. reptans leaves were investigated. Different fractions (ethanol, ethyl acetate, and hexane) of I. reptans leaves were evaluated for their scavenging activity on DPPH radicals, whereas their reducing potential was investigated by cupric reducing antioxidant capacity (CuPRAC), total antioxidant, and reducing power assays. The antidiabetic potential was investigated by their inhibition effect on a-glucosidase. Total phenolic and flavonoid contents of I. reptans leaves were solvent dependent. Ethyl acetate contained the highest phenolic content, followed by ethanol and hexane fractions. However, for flavonoid content, the order was ethanol > ethyl acetate > hexane. All fractions showed DPPH scavenging activity in a concentration-dependent manner, with activities weaker than standards ascorbic acid and BHT, in the order of ethanol > ethyl acetate > hexane. All fractions showed reducing capacity, but only hexane and ethanol fractions of I. reptans leaves showed inhibition on a-glucosidase, with hexane showed more potent inhibition compared to acarbose. The study also found that fractions of I. reptans inhibit lipase and trypsin, enzymes related to lipid metabolism. Findings in this study offer a prospect for I. reptans leaves as a functional food source for antioxidant, antidiabetic, and anti-obesity purposes.

Isolation of Phenolic Acids from Land Kale (Ipomoea reptans Poir) and Antioxidant Activity

“…The total phenolic acid of the ethanol extract of land kale obtained was 71.2420 ± 0.0791 mg/g GAE. The total phenolic results obtained in this study exceeded those reported in prior research by Mariani et al [22], where the value was 31 ± 0.849 mg/g GAE, and in the research conducted by Kurniawan et al [2], which reported a value of 0.86 mg/g GAE.…”

“…Land kale contains vitamins and minerals that are good for human health and have secondary metabolite compounds in the form of phenolic acids, flavonoids, saponins, alkaloids, steroids, and triterpenoids [1]. Based on the content of secondary metabolites, kale plants are reported to have several activities, including antioxidant [2], anti-diabetic [3], anti-cancer [4], antiinflammatory, anti-microbial [5], anti-ulcers [6], and hypolipidemic agents [7].…”

The Immune-Stimulating and Anti-Diabetic Effects of Allium hookeri Leaves Grown in a Plant Factory with Artificial Lights in Immunosuppressed Obese C57BL/6 Mice

“…The IC 50 value of the α-glucosidase inhibition activity of Ipomoea reptans leaf ethanolic extract was 0.26 mg/mL, and the value was 47.8 μg/mL in Olax imbricata Roxb. root methanol extract [ 32 , 33 ]. The IC 50 values of the α-glucosidase inhibitory activity of K. africana aqueous and ethanolic extracts were 486.92 and 157.99 μg/mL [ 30 ].…”

The Stem Infusate and Ethanol Extract of Physalis angulata Inhibitory Activities against a-Glucosidase and Xanthine Oxidase

“…Determination of DPPH radical scavenging activity was conducted according to a previous method. 13 DPPH solution in ethanol (0.6 mM, 3 mL) was mixed with samples of different concentrations (1 mL in ethanol). The reaction mixture was incubated in the dark at room temperature for 30 mins, thereafter the absorbance was measured at 517 nm.…”