Antioxidant, antibacterial and antiacetylcholinesterase activities of abietic acid from Isodon wightii (Bentham) H. Hara

Abstract

Objective:Isodon is an important genus of the family Lamiaceae which contains diterpenoids of diverse functionalities. The present study attempted with isolation, structure elucidation and bioactivity evaluation of isolated compound from the leaves of Isodon wightii (Bentham) H. Hara. Methods: Petroleum ether extract of powdered leaves was obtained and concentrated in vacuo at 45ºC. Compound was isolated using silica gel column chromatography (60-120 mesh). Antioxidant activity of isolated compound was tested for DPPH free radical and hydroxyl radical scavenging, inhibition of linoleic acid peroxidation and metal ion chelation activities. Antibacterial activity was tested against six pathogenic bacteria by micro broth dilution method and in vitro antiacetyl cholinesterase activity of isolated compound was also studied. Results: Abietane diterpenoid, abietic acid was isolated from the leaves of I. wightii and the structure has been elucidated on the basis of spectroscopic analysis. Abietic acid isolated from petroleum ether extract of leaves showed moderate DPPH free radical, hydroxyl radical scavenging and less inhibition of linoleic acid peroxidation and metal ion chelating activities. Antibacterial activity of abietic acid against Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Proteus mirabilis, Proteus vulgaris and Staphylococcus aureus was found in the range of 340-980 µg/mL. Antiacetylcholinesterase activity of abietic acid was less when compared with positive control. Conclusion: Abietic acid showed concentration dependent antioxidant, antibacterial and antiacetylcholinesterase activities.

Novel aqueous oil-in-water emulsions containing extracts of natural coniferous resins are strongly antimicrobial against enterobacteria, staphylococci and yeasts, as well as on bacterial biofilms

“…; Gogulramnath et al . ). These main resin acids are not, however, water soluble and will not be transferred into aqueous emulsions or dispersions as the case is with the oxidized resin acids.…”

“…The oxidized resin acids occurred in emulsions in low concentrations, in range of some hundreds of ppms at most. In earlier literature, the main resin acids, like abietic acid and dehydroabietic acid, are shown to be antimicrobial (S€ oderberg et al 1990;Leandro et al 2014;Gogulramnath et al 2015). These main resin acids are not, however, water soluble and will not be transferred into aqueous emulsions or dispersions as the case is with the oxidized resin acids.…”

Obtaining salts of resin acids from Cuban pine by metathesis reactions

“…Studies reported in the literature for other pine species have shown that abietic acid and dehydroabietic acid have a strong antimicrobial activity against gram-positive bacteria, being dehydroabietic acid the most effective of all simple resin acids [4,[15][16][17][18][19][20]. The differences in antibacterial efficacy detected among the different reports found in the literature are due to the fact that the studied rosins come from different species and the growth conditions, the recovery processes, the concentrations of the different resin acids, the neutral fraction, the storage and age are factors that affect the quality of rosin.…”

Hepatoprotective and cytotoxic activities of abietic acid from Isodon wightii (bentham) H. hara

“…Yellowish amorphous powder (82 mg) was obtained at 70 th h. Based on the spectral studies (IR, 1 H NMR, 13 C NMR, COSY, HSQC, and HMBC), the compound structure was predicted. [ 5 ]…”

“…[ 1 2 3 4 ] In our previous work, we reported free radical scavenging, antibacterial and antiacetylcholinesterase activities of abietane diterpenoid, abietic acid (ABA) from Isodon wightii . [ 5 ] Therefore, the present study was aimed to test hepatoprotective and cytotoxic potentials of ABA.…”