Antioxidant, Anti-inflammatory, and Antiproliferative Activities of <i>Taxillus sutchuenensis</i>

Liu, Chia-Yu; Lin, Ying-Chih; Deng, Jeng-Shyan; Liao, Jung-Chun; Peng, Wen-Huang; Huang, Guan‐Jhong

doi:10.1142/s0192415x12500267

Cited by 25 publications

(21 citation statements)

References 8 publications

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“…FRAP values of P. minus fractions were linearly correlated to TPC (FRAP versus TPC, r = 0.83, P < 0.05). Since EtOAc typically extracts polyphenolic compounds [28], F7 most probably contains polyphenols with high antioxidant capacity since polyphenols act as electron donors to stabilize free radicals [29]. Plant extracts that contained high concentrations of phenolics demonstrate high antioxidant effects in vitro [30].…”

Section: Resultsmentioning

confidence: 99%

Apoptosis Induction byPolygonum minusIs Related to Antioxidant Capacity, Alterations in Expression of Apoptotic-Related Genes, and S-Phase Cell Cycle Arrest in HepG2 Cell Line

Ghazali

Al-Naqeb

Selvarajan

et al. 2014

BioMed Research International

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Polygonum minus (Polygonaceae) is a medicinal herb distributed throughout eastern Asia. The present study investigated antiproliferative effect of P. minus and its possible mechanisms. Four extracts (petroleum ether, methanol, ethyl acetate, and water) were prepared by cold maceration. Extracts were subjected to phytochemical screening, antioxidant, and antiproliferative assays; the most bioactive was fractionated using vacuum liquid chromatography into seven fractions (F1–F7). Antioxidant activity was measured via total phenolic content (TPC), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and ferric reducing antioxidant power (FRAP) assays. Antiproliferative activity was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Most active fraction was tested for apoptosis induction and cell cycle arrest in HepG2 cells using flow cytometry and confocal microscopy. Apoptotic-related gene expression was studied by RT-PCR. Ethyl acetate extract was bioactive in initial assays. Its fraction, F7, exhibited highest antioxidant capacity (TPC; 113.16 ± 6.2 mg GAE/g extract, DPPH; EC50: 30.5 ± 3.2 μg/mL, FRAP; 1169 ± 20.3 μmol Fe (II)/mg extract) and selective antiproliferative effect (IC50: 25.75 ± 1.5 μg/mL). F7 induced apoptosis in concentration- and time-dependent manner and caused cell cycle arrest at S-phase. Upregulation of proapoptotic genes (Bax, p53, and caspase-3) and downregulation of antiapoptotic gene, Bcl-2, were observed. In conclusion, F7 was antiproliferative to HepG2 cells by inducing apoptosis, cell cycle arrest, and via antioxidative effects.

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Section: Resultsmentioning

confidence: 99%

Apoptosis Induction byPolygonum minusIs Related to Antioxidant Capacity, Alterations in Expression of Apoptotic-Related Genes, and S-Phase Cell Cycle Arrest in HepG2 Cell Line

Ghazali

Al-Naqeb

Selvarajan

et al. 2014

BioMed Research International

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“…sutchuenensis , is used in folk medicine. These two medicinal plants are commonly used to treat diseases, such as rheumatism, hypertension, and fetal irritability 34,35 . The recorded hosts of T .…”

Section: Introductionmentioning

confidence: 99%

Gene losses and partial deletion of small single-copy regions of the chloroplast genomes of two hemiparasitic Taxillus species

Zhou

Chen

et al. 2017

Sci Rep

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Numerous variations are known to occur in the chloroplast genomes of parasitic plants. We determined the complete chloroplast genome sequences of two hemiparasitic species, Taxillus chinensis and T. sutchuenensis, using Illumina and PacBio sequencing technologies. These species are the first members of the family Loranthaceae to be sequenced. The complete chloroplast genomes of T. chinensis and T. sutchuenensis comprise circular 121,363 and 122,562 bp-long molecules with quadripartite structures, respectively. Compared with the chloroplast genomes of Nicotiana tabacum and Osyris alba, all ndh genes as well as three ribosomal protein genes, seven tRNA genes, four ycf genes, and the infA gene of these two species have been lost. The results of the maximum likelihood and neighbor-joining phylogenetic trees strongly support the theory that Loranthaceae and Viscaceae are monophyletic clades. This research reveals the effect of a parasitic lifestyle on the chloroplast structure and genome content of T. chinensis and T. sutchuenensis, and enhances our understanding of the discrepancies in terms of assembly results between Illumina and PacBio.

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“…There are a total of 51 species of parasitic loranthus, of which 23 are distributed in Guangxi of China [5]. It is also called “Sangjisheng” in China and grows by parasitizing other plants like Aceraceae, Anacardiaceae, Euphorbiaceae, Fabaceae, Fagaceae, Juglandaceae, Moraceae, Rosaceae, and Rutaceae [6]. In plants, developmental processes such as seed germination, seedling development, leaf development and flowering are always affected by various environmental stresses, such as drought, high salinity, and high or low temperatures [7–9].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis of Taxillusi chinensis (DC.) Danser Seeds in Response to Water Loss

Wei

Pan

et al. 2017

PLoS ONE

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BackgroundTaxillus chinensis (DC.) Danser, the official species of parasitic loranthus that grows by parasitizing other plants, is used in various traditional Chinese medicine prescriptions. ABA-dependent and ABA-independent pathways are two major pathways in response to drought stress for plants and some genes have been reported to play a key role during the dehydration including dehydration-responsive protein RD22, late embryogenesis abundant (LEA) proteins, and various transcription factors (TFs) like MYB and WRKY. However, genes responding to dehydration are still unknown in loranthus.Methods and ResultsInitially, loranthus seeds were characterized as recalcitrant seeds. Then, biological replicates of fresh loranthus seeds (CK), and seeds after being dehydrated for 16 hours (Tac-16) and 36 hours (Tac-36) were sequenced by RNA-Seq, generating 386,542,846 high quality reads. A total of 164,546 transcripts corresponding to 114,971 genes were assembled by Trinity and annotated by mapping them to NCBI non-redundant (NR), UniProt, GO, KEGG pathway and COG databases. Transcriptome profiling identified 60,695, 56,027 and 66,389 transcripts (>1 FPKM) in CK, Tac-16 and Tac-36, respectively. Compared to CK, we obtained 2,102 up-regulated and 1,344 down-regulated transcripts in Tac-16 and 1,649 up-regulated and 2,135 down-regulated transcripts in Tac-36 by using edgeR. Among them some have been reported to function in dehydration process, such as RD22, heat shock proteins (HSP) and various TFs (MYB, WRKY and ethylene-responsive transcription factors). Interestingly, transcripts encoding ribosomal proteins peaked in Tac-16. It is indicated that HSPs and ribosomal proteins may function in early response to drought stress. Raw sequencing data can be accessed in NCBI SRA platform under the accession number SRA309567.ConclusionsThis is the first time to profile transcriptome globally in loranthus seeds. Our findings provide insights into the gene regulations of loranthus seeds in response to water loss and expand our current understanding of drought tolerance and germination of seeds.

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Antioxidant, Anti-inflammatory, and Antiproliferative Activities of Taxillus sutchuenensis

Cited by 25 publications

References 8 publications

Apoptosis Induction byPolygonum minusIs Related to Antioxidant Capacity, Alterations in Expression of Apoptotic-Related Genes, and S-Phase Cell Cycle Arrest in HepG2 Cell Line

Apoptosis Induction byPolygonum minusIs Related to Antioxidant Capacity, Alterations in Expression of Apoptotic-Related Genes, and S-Phase Cell Cycle Arrest in HepG2 Cell Line

Gene losses and partial deletion of small single-copy regions of the chloroplast genomes of two hemiparasitic Taxillus species

Transcriptome Analysis of Taxillusi chinensis (DC.) Danser Seeds in Response to Water Loss

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