Antioxidant and α-glucosidase inhibitory properties of soluble melanins from the fruits of Vitex mollis Kunth, Randia echinocarpa Sessé et Mociño and Crescentia alata Kunth

Cuevas‐Juárez, Esmeralda; Yuriar-Arredondo, Korinthia Yamileth; Pío-León, Juan Fernando; Montes‐Ávila, Julio; López‐Ángulo, Gabriela; Díaz‐Camacho, Sylvia Páz; Delgado‐Vargas, Francisco

doi:10.1016/j.jff.2014.04.016

Cited by 36 publications

(29 citation statements)

References 38 publications

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“…30 mesh. The flour was stored at −20°C and protected from light until its use (Cuevas‐Juárez et al, ).…”

Section: Methodsmentioning

confidence: 99%

“…The suspension was centrifuged at 20,000 g/20°C for 15 min; the supernatant was recovered, stored at −70°C, and freeze‐dried. Besides, the raw water‐soluble melanins at boiling temperature were prepared as above described but mixing and agitation were carried out at boiling temperature (Cuevas‐Juárez et al, ).…”

Section: Methodsmentioning

confidence: 99%

“…The freeze‐dried raw‐soluble melanin (0.5 g) was suspended in 10 ml of deionized water and dialyzed through a pre‐hydrated 12 kDa cellulose membrane (Sigma‐Aldrich, Seelze, Germany), using a flask with 300 ml of deionized water; dialysis was carried out for 4 days at 5°C and exchanging the water in the flask twice a day. The dialysis retentate was recovered, stored at −70°C, and freeze‐dried to obtain the soluble melanins at room (SMRT) and boiling (SMBT) temperatures (Cuevas‐Juárez et al, ). The extractions were done by triplicate and yield (%) was calculated for each SM.…”

Section: Methodsmentioning

confidence: 99%

“…Randia echinocarpa (Rubiaceae) is native to Mexico, known as “papache,” used in Mexican traditional medicine to treat diseases (e.g., cancer, malaria, and diabetes) and ailments of organs/systems (e.g., kidney, respiratory, circulatory, and gastrointestinal); and the aqueous extract of the R. echinocarpa fruit shows antioxidant and antimutagenic properties (Cano‐Campos et al, ; Santos‐Cervantes, Ibarra‐Zazueta, Loarca‐Piña, Paredes‐López, & Delgado‐Vargas, ). This fruit contains melanins and their structures were characterized: the water‐soluble melanins (SM) by UV–vis and IR spectroscopy (Cuevas‐Juárez et al, ); and the insoluble melanins by spectroscopic (UV–vis, IR, 1 H NMR, and 13 C NMR), thermogravimetric (TGA), and elemental analysis (Montes‐Avila et al, ); these studies suggested that solubility of the R. echinocarpa SM is associated with carbohydrates bound to the phenolic structures of melanins, characteristic that remains to be demonstrated. The R. echinocarpa SM have high antioxidant and α‐glucosidase inhibitory values (Cuevas‐Juárez et al, ), and their use as food or supplement could be better supported by improving their structural and toxicological characterization.…”

Section: Introductionmentioning

confidence: 99%

“…This fruit contains melanins and their structures were characterized: the water‐soluble melanins (SM) by UV–vis and IR spectroscopy (Cuevas‐Juárez et al, ); and the insoluble melanins by spectroscopic (UV–vis, IR, 1 H NMR, and 13 C NMR), thermogravimetric (TGA), and elemental analysis (Montes‐Avila et al, ); these studies suggested that solubility of the R. echinocarpa SM is associated with carbohydrates bound to the phenolic structures of melanins, characteristic that remains to be demonstrated. The R. echinocarpa SM have high antioxidant and α‐glucosidase inhibitory values (Cuevas‐Juárez et al, ), and their use as food or supplement could be better supported by improving their structural and toxicological characterization. Thus, this study aims to establish structural characteristics associated with the aqueous solubility of the R. echinocarpa soluble melanins, and to establish its acute and sub‐acute toxicity in mice.…”

Section: Introductionmentioning

confidence: 99%

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Soluble melanins of the Randia echinocarpa fruit – Structural characteristics and toxicity

et al. 2019

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The water-soluble melanins (SM) of Randia echinocarpa fruit possess interesting biological activities and have been scarcely characterized. In this study, SM were obtained at boiling (SMBT) and room (SMRT) temperatures and characterized by UV-Vis, IR, thermogravimetric analysis, and GC-MS of the hydrolysis products of the SM; besides, the solid-state 13 C NMR, elemental analysis, and acute and sub-acute toxicity of the SMBT were determined. SMBT and SMRT contain organic acids and carbohydrates and their spectroscopic signals and thermograms were similar, but the SMBT yield was higher.The SMBT were characterized by their elemental composition (C 48.260 ± 0.011%, N 3.693 ± 0.009% and H 6.093 ± 0.076%) consistent with the presence of aromatic rings and eumelanins, degradation temperature at 300°C, 13 C NMR signals supporting melanin-bonding with carbohydrates and organic acids, and innocuity in Balb/C mice (acute assay, LD 50 > 5 g/kg b.w.; sub-acute assay, no lethality at 500 mg/kg b.w. for 30 days). Practical applicationsThe consumption of melanins has been associated with health benefits because of their biological activities (e.g., antioxidant, immunostimulatory, UV-and radiationprotective). Randia echinocarpa is employed in Mexican traditional medicine against chronic degenerative diseases (e.g., cancer and diabetes) and ailments of organs (e.g., kidney and lung) and systems (e.g., circulatory and gastrointestinal). The R. echinocarpa fruit contains water-soluble melanins (SM) that inhibit carbohydrate-digestive enzymes and show high antioxidant activity; thus, SM could be useful for the prevention and treatment of diabetes. This study showed that the SM structure contains melanin-bonding organic acids and carbohydrates, which could be associated with the SM solubility and higher yield, and that SMBT were innocuous in the acute and sub-acute assays in mice. Thus, the R. echinocarpa SMBT could be used as safe potential ingredients to develop functional products. K E Y W O R D Sdietary supplements, elemental analysis, functional food, melanin, solid-state carbon-13 magnetic resonance spectroscopy, thermogravimetry, toxicity 2 of 12 | GIL-AVILÉS et AL.

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“…30 mesh. The flour was stored at −20°C and protected from light until its use (Cuevas‐Juárez et al, ).…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Soluble melanins of the Randia echinocarpa fruit – Structural characteristics and toxicity

et al. 2019

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Modeling the growth kinetics of cell suspensions of Randia echinocarpa (Rubiaceae) and characterization of their bioactive phenolic compounds

Aguilar-Camacho,

Gómez-Sánchez,

Cruz-Mendívil

et al. 2023

Plant Cell Tiss Organ Cult

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The fruit of Randia echinocarpa is commonly used in the Mexican tradicional medicine to treat different diseases and ailments. So, the establishment of cell suspension cultures (CSC) is a required alternative to obtain bioactive compounds from this plant. Moreover, good kinetic models are necessary good processes control and simulation of plant cell cultures. Therefore, the objective of this study was to model and estimate the growth kinetics of the CSC of R. echinocarpa, as well as characterize their phenolic pro le by ultra performance liquid chromatography coupled to mass spectrometry. During the 34 d of culture, CSC of R. echinocarpa reached a dry cell biomass concentration of 15.16 g/L at day 20. The maximum speci c growth rate ( ) was 0.15 d − 1 , with a duplication time ( ) of 4.62 d. The Logistic model proposed adequately predicts the cell growth changes during the culture and the maximum dry cell content the culture medium can sustain (≈ 13.63 g/L). Ten phenolic compounds were identi ed in the biomass and four in the supernatants. The major phenolic compound in the biomass was chlorogenic acid (CA), with a concentration of 828.6 µg/g at day 20. In the lyophilized supernatant, the major phenolic compound was salicylic acid (SA) with a concentration of 172.7 µg/g at day 30. The production of CA was a growth-dependent process in contrast to the concentration of SA in the media. Our results indicate CSC of R. echinocarpa could be a sustainable source for the production of bioactive compounds such as CA and SA.

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Untargeted metabolomic analysis of Randia echinocarpa cell cultures treated with L-Tyrosine

Aguilar-Camacho,

Gómez-Sánchez,

Cruz-Mendívil

et al. 2024

Plant Cell Tiss Organ Cult

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Antioxidant and α-glucosidase inhibitory properties of soluble melanins from the fruits of Vitex mollis Kunth, Randia echinocarpa Sessé et Mociño and Crescentia alata Kunth

Cited by 36 publications

References 38 publications

Soluble melanins of the Randia echinocarpa fruit – Structural characteristics and toxicity

Soluble melanins of the Randia echinocarpa fruit – Structural characteristics and toxicity

Modeling the growth kinetics of cell suspensions of Randia echinocarpa (Rubiaceae) and characterization of their bioactive phenolic compounds

Untargeted metabolomic analysis of Randia echinocarpa cell cultures treated with L-Tyrosine

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