Antioxidant and hepatoprotective potential ofAegle marmelos Correa.against CCl₄-induced oxidative stress and early tumor events

Abstract

The antioxidant properties and inhibitory effect on early tumor promoter markers of A. marmelos (25 and 50 mg/Kg b. wt. orally) have been evaluated. Male Wistar rats were pre-treated for seven consecutive days with A. marmelos prior to CCl 4 (1 mL Kg 21 body weight p. o., in corn oil [1:1 v/v]) treatment. Pre-treatment with A. marmelos suppressed lipid peroxidation (LPO), xanthine oxidase (XO) and release of serum toxicity marker enzymes viz, SGOT, LDH, SGPT dose-dependently and significantly (p , 0.001). Hepatic antioxidant status viz, reduced glutathione (GSH), glutathione reductase (GR), glutathione peroxidase (GPx), quinone reductase (QR), catalase (CAT) were concomitantly restored in A. marmelos-treated groups (p , 0.001). In addition, A. marmelos pretreatment also prevented the CCl 4 -enhanced ornithine decarboxylase (ODC) and hepatic DNA synthesis significantly (p , 0.001). In conclusion, carbon tetrachloride-induced liver toxicity was strikingly attenuated by A. marmelos treatment and the study gives some insight into the mechanisms involved in diminution of free radical generating toxicants and enhancement of the antioxidant armory, hence preventing further tissue damage, injury and hyperproliferation.Thus, these findings indicate that A. marmelos attenuates CCl 4 -mediated hepatic oxidative stress, toxicity, tumor promotion and subsequent cell proliferation response in Wistar rats.

Phytochemistry and medicinal uses of the bael fruit (Aegle marmelos Correa): A concise review

“…Multiple preclinical studies in the recent past have shown that oral administration of bael protected rats and mice against the CCl 4 -induced hepatic damage (Khan & Sultana, 2009;Rajasekaran, 2009;Singh & Rao, 2008). Oral feeding of bael (25 and 50 mg/kg b. wt.…”

Attenuation of CCl4 Induced Oxidative Stress, Immunosuppressive, Hepatorenal Damage by Fucoidan in Rats

“…At the molecular level, CCl 4 activates TNF-α, TGF-β1, and IL-6 production that appear to direct the cell toward destruction or fibrosis, while IL-10 counteract the liver fibrogensis. In this context, Fue et al and Tan et al concluded the elevation the inflammatory cytokines have a key role in pathogenesis of liver fibrosis and activation of HSCs [14][15][16][17][18][19][20][21][22].…”

“…In the same aspect, Park et al concluded that fucoidan applies anti-inflammatory effects by inhibiting the expression of pro-inflammatory cytokines in vitro and in vivo, together with a restricted antibacterial effect in vivo. Furthermore, fucoidan enhanced the production of pro-inflammatory cytokines, IL-6, IL-8 and TNF-α in human neutrophil and delay neutrophil apoptosis [17][18][19][20][21][22][23][24][25][26].…”

Antioxidant effect of functionalized alkyl-organotellurides: a studyin vitro

“…Oxidative stress is characterized by a significantly increased concentration of intracellular oxidizing species, such as reactive oxygen species (ROS), and is often accompanied by the simultaneous loss of antioxidant defense capacity 15,16 . In pathologic conditions an overproduction of ROS can occur 17 . Besides that, ROS overproduction has been implicated in the installation and/or progression of a variety of human diseases 18,19 including diabetes 20 , cancer 21 , and various neurodegenerative diseases 22 .…”