Antioxidant and functional properties of cowhide collagen peptides

Abstract

In the present study, antioxidant activities and functional properties of cowhide collagen antioxidant peptides (CCAPs) with different molecular weight (MW) were investigated. The optimum preparation conditions of CCAPs were hydrolysis time of 1.53 hr, temperature of 54.9 °C, pH 7.38, and neutral enzyme to trypsin ratio of 0.048 g: 0.016 g according to single factor test and response surface methodology (RSM). Three fractions (CCAP‐I, CCAP‐II, and CCAP‐III) were obtained by ultrafiltration and lyophilization. Antioxidant activities revealed that CCAP‐III had high reducing power activity (0.323 ± 0.035) and scavenging effect on 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) radicals (64.30 ± 5.99%), 2,2‐azino‐bis‐(3‐ethylbenzothiazoline)‐6‐sulfonic acid (ABTS) radicals (75.25 ± 3.14%), and hydroxyl radicals (68.26 ± 6.74%) compared to the other fractions. In addition, LC‐MS/MS analysis showed that Ala‐Gly‐Glu‐Arg, Gly‐Ile‐Ala‐Gly‐Glu‐Arg, Gly‐Pro‐Ala‐Gly‐Pro‐Ala‐Gly‐Pro‐Arg, Gly‐Val‐Val‐Gly‐Pro‐Glu‐Gly‐Ala‐Arg and Gly‐Phe‐Ser‐Gly‐Leu‐Asp‐Gly‐Ala‐Lys were the major peptides of CCAP‐III. CCAP‐III showed good hygroscopicity (HYG), water holding capacity (WHC), and oil holding capacity (OHC) when compared with CCAP‐I and CCAP‐II. However, CCAP‐II has great emulsifying properties, and CCAP‐I has excellent foaming properties. Therefore, CCAPs can be used as a promising source of functional peptides with antioxidant properties. Practical Application This study demonstrated the peptides of cowhide collagen has superior antioxidant and functional properties. This study provided a scientific basis for the preparation of antioxidant peptides from cowhide collagen.

Using RSM for Optimum of Optimum Production of Peptides from Edible Bird’s Nest By-Product and Characterization of Its Antioxidant’s Properties

“…When the water/material ratio was 30.0, DH reached the highest 9.43 ± 0.30%. While the water/material ratio exceeded 30.0, the substrate concentration was too low, which reduced the collision probability between protease and substrate, partially inhibited the enzymatic hydrolysis reaction, and reduced the DH [ 31 ]. Therefore, the optimum water/material ratio was 30.0.…”

Solid-state fermentation produces greater stearidonic acid levels in genetically engineered Mucor circinelloides

“…The ANOVA analysis described in Table 3 generated high F-values (91Á01, 296Á61, 18Á29 and 49Á62) and low P-values (<0Á0001, <0Á0001, 0Á0005 and <0Á0001), respectively, thus indicating that these models were statistically significant. This indicates that these regression equations can be used to accurately determine the relationship between the model used and experimental variables (glucose concentration; ammonium tartrate concentration; temperature) used in the optimization study (Xie et al 2021b). The P-values for lack of fit of 0Á6313, 0Á9340, 0Á1442 and 0Á3616 were all insignificant (P > 0Á05), indicating that the test and regression model values were well fitted.…”

“…This indicates that these regression equations can be used to accurately determine the relationship between the model used and experimental variables (glucose concentration; ammonium tartrate concentration; temperature) used in the optimization study (Xie et al . 2021b). The P ‐values for lack of fit of 0·6313, 0·9340, 0·1442 and 0·3616 were all insignificant ( P > 0·05), indicating that the test and regression model values were well fitted.…”

“…To select the optimal experimental conditions, an optimization process was performed using the RSM (Hadidi et al 2019). The BBD approach was used to investigate the effect and relationship between three independent variables (X 1 , glucose concentration; X 2 , ammonium tartrate concentration; X 3 , temperature) on lipid production in McD15D (Xie et al 2021b). This BBD study was coded at three levels (−1, 0, 1) using four dependent variables: Y 1 , spores SDA content; Y 2 , spores TFA content; Y 3 , SDA mycelium content; and Y 4 , mycelium TFA content.…”

Quinoa husk peptides reduce melanin content via Akt signaling and apoptosis pathways

“…Next, the different fractions were collected. Subsequently, the fractions were freeze-dried and the TYR inhibition rate, DPPH scavenging rate, 31 reducing activity, 32 and chelation rate 33 were determined.…”