Antioxidant and Free Radical-Scavenging Activity of Choto-san and Its Related Constituents

Mahakunakorn, Pramote; Tohda, Michihisa; Murakami, Yukihisa; Matsumoto, Kinzo; Watanabe, Hiroshi

doi:10.1248/bpb.27.38

Cited by 106 publications

(88 citation statements)

References 34 publications

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“…The superoxide radical scavenging activity in the PMS/ NADH system was assayed as described previously. 15) Briefly, reaction mixtures contained various concentrations of the test compounds, PMS (15 mM), NADH (73 mM) and NBT (50 mM) in a phosphate buffer (20 mM, pH 7.4). After incubating for 5 min at ambient temperature, the color was measured at 560 nm against blank samples.…”

Section: Abtsmentioning

confidence: 99%

Antioxidative Activities of Galloyl Glucopyranosides from the Stem-Bark ofJuglans mandshurica

Ngoc

Hung

Thương

et al. 2008

Bioscience, Biotechnology, and Biochemistry

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Section: Abtsmentioning

confidence: 99%

Antioxidative Activities of Galloyl Glucopyranosides from the Stem-Bark ofJuglans mandshurica

Ngoc

Hung

Thương

et al. 2008

Bioscience, Biotechnology, and Biochemistry

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“…Moreover, pharmacological studies have reported that CTS prevents the occurrence of stroke, prolongs the life span of stroke-prone spontaneously hypertensive rats (4,5), and reduces tissue damage associated with ischemia reperfusion. Furthermore, polyphenolic compounds and some alkaloid components have been reported to play an important role in cytoprotection against tissue damage mediated by oxidative stress (6) and excessive excitatory synaptic transmission (7,8). However, very little information is available about the mechanisms underlying the therapeutic effects of CTS in stroke patients.…”

Section: Introductionmentioning

confidence: 99%

Chotosan, a Kampo Formula, Ameliorates Chronic Cerebral Hypoperfusion-Induced Deficits in Object Recognition Behaviors and Central Cholinergic Systems in Mice

et al. 2007

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Abstract. We previously demonstrated that the Kampo formula chotosan (CTS) ameliorated spatial cognitive impairment via central cholinergic systems in a chronic cerebral hypoperfusion (P2VO) mouse model. In this study, the object discrimination tasks were used to determine if the ameliorative effects of CTS on P2VO-induced cognitive deficits are a characteristic pharmacological profile of this formula, with the aim of clarifying the mechanisms by which CTS enhances central cholinergic function in P2VO mice. The cholinesterase inhibitor tacrine (THA) and Kampo formula saikokeishito (SKT) were used as controls. P2VO impaired object discrimination performance in the object recognition, location, and context tests. Daily administration of CTS (750 mg / kg, p.o.) and THA (2.5 mg / kg, i.p.) improved the object discrimination deficits, whereas SKT (750 mg / kg, p.o.) did not. In ex vivo assays, tacrine but not CTS or SKT inhibited cortical cholinesterase activity. P2VO reduced the mRNA expression of m 3 and m 5 muscarinic receptors and choline acetyltransferase but not that of other muscarinic receptor subtypes in the cerebral cortex. Daily administration of CTS and THA but not SKT reversed these expression changes. These results suggest that CTS and THA improve P2VO-induced cognitive impairment by normalizing the deficit of central cholinergic systems and that the beneficial effect on P2VO-induced cognitive deficits is a distinctive pharmacological characteristic of CTS.

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“…[11] Different volumes of ECME (0-100 µg/ml) were mixed with ethanol and 50 µl of DPPH was added and incubated for 2 minutes, followed by absorbance at 517 nm. The percentage of DPPH radical scavenging activity was calculated using formula: % of …”

Section: Dpph Radical Scavenging Assaymentioning

confidence: 99%

ASSESSMENT OF THE ANTIOXIDANT ACTIVITY AND PHYTOCHEMICAL ANALYSIS OF A LICHEN, EVERNIASTRUM CIRRHATUM (FR.) HALE ex. SIPMAN

Mandal¹

2017

WJPPS

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Free radicals generated in system induce oxidative stress related diseases. Recent research is focused on plants and phytocompounds to avoid various side effects. The present study is aimed to evaluate the free radical scavenging potential along with phytochemical screening of the 70% methanolic extract of a lichen, Everniastrum cirrhatum (Fr.) Hale (ECME). ECME was tested for its in vitro total antioxidant capacities and various other reactive oxygen and nitrogen species (ROS, RNS) scavenging potentials. For further insight, phytochemical screening through qualitative and quantitative analyses along with HPLC standardization were performed. It was observed that ECME showed total antioxidant capacity with TEAC value of 0.384 ± 0.0074 as compared to Trolox. The lichen also showed excellent ROS and RNS scavenging capacities as it scavenges hydroxyl, superoxide, hypochlorous acid, nitric oxide and peroxynitrite radicals with IC 50 values of 208. 09 ± 6.36, 142.26 ± 3.17, 150.07 ± 3.01, 118.19 ± 5.99 and 3037.07 ± 222.43 µg/ml respectively. Singlet oxygen is also scavenged but to a lesser extent by ECME. Phytochemical analyses of ECME showed greater presence of alkaloids, phenolics, flavonoids like compounds as compared to other phytocompounds.Moreover HPLC analysis of ECME revealed the presence of antioxidant phytochemicals like purpurin, usnic acid, tannic acid, reserpine and rutin at various retention times. The in vitro results show that ECME possesses promising antioxidant and free radical scavenging

show abstract

Antioxidant and Free Radical-Scavenging Activity of Choto-san and Its Related Constituents

Cited by 106 publications

References 34 publications

Antioxidative Activities of Galloyl Glucopyranosides from the Stem-Bark ofJuglans mandshurica

Antioxidative Activities of Galloyl Glucopyranosides from the Stem-Bark ofJuglans mandshurica

Chotosan, a Kampo Formula, Ameliorates Chronic Cerebral Hypoperfusion-Induced Deficits in Object Recognition Behaviors and Central Cholinergic Systems in Mice

ASSESSMENT OF THE ANTIOXIDANT ACTIVITY AND PHYTOCHEMICAL ANALYSIS OF A LICHEN, EVERNIASTRUM CIRRHATUM (FR.) HALE ex. SIPMAN

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