Antioxidant and cytotoxic activities of naturally occurring phenolic and related compounds: A comparative study

Rao, Yerra Koteswara; Geethangili, Madamanchi; Fang, Shih-Hua; Tzeng, Yew‐Min

doi:10.1016/j.fct.2007.03.012

Cited by 128 publications

(92 citation statements)

References 32 publications

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“…Critical steps in the signal transduction cascade are sensitive to oxidants and antioxidants (Sen and Packer 1996). Studies have shown that plant derived antioxidants showed cytotoxicity towards tumor cells (Rao et al 2007). In vitro antioxidant studies carried out with two active fractions of the extract showed potential free radical scavenging activity.…”

Section: Discussionmentioning

confidence: 99%

Assessment of the in vitro cytotoxicity and in vivo anti-tumor activity of the alcoholic stem bark extract/fractions of Mimusops elengi Linn.

et al. 2015

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Various parts of Mimusops elengi Linn. (Sapotaceae) have been used widely in traditional Indian medicine for the treatment of pain, inflammation and wounds. The study was conducted to explore the use of stem bark of M. elengi on pharmacological grounds and to evaluate the scientific basis of cytotoxic and anti-tumor activity. Extract/fractions were prepared and in vitro cytotoxicity was assessed using SRB assay. Most effective fractions were subjected to fluorescence microscopy based acridine orange/ethidium bromide (AO/EB) and Hoechst 33342 staining to determine apoptosis induction and DNA fragmentation assay. Comet and micronuclei assay were performed to assess genotoxicity. Cell cycle analysis was also performed. In vivo anti-tumor potential was evaluated by Ehrlich ascites carcinoma (EAC) model in mice. The alcoholic stem bark extract of M. elengi along with four fractions showed potential in vitro cytotoxicity in SRB assay. Of these, dichloromethane and ethyl acetate fractions were selected for further studies. The fractions revealed apoptosis inducing potential in AO/EB and Hoechst 33342 staining, which was further confirmed by DNA fragmentation assay. Genotoxic potential was revealed by comet and micronuclei assay. Fractions also exhibited specific cell cycle inhibition in G 0 /G 1 phase. In EAC model, ethyl acetate fraction along with the standard (cisplatin) effectively reduced the increase in body weight compared to control and improved mean survival time. Both fractions were able to restore the altered hematological and biochemical parameters. Hence, M. elengi stem bark may be a possible therapeutic candidate having cytotoxic and anti-tumor potential.

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Section: Discussionmentioning

confidence: 99%

Assessment of the in vitro cytotoxicity and in vivo anti-tumor activity of the alcoholic stem bark extract/fractions of Mimusops elengi Linn.

et al. 2015

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“…3,4 ROT tarafından oluşturulan oksidatif hasarın lipitlerinin, proteinleri ve nükleik asitleri etkileyerek kanser, diyabet, nörodejeneratif (Alzheimer ve Parkinson hastalığı gibi) ve kardiyovasküler pek çok hastalığa neden olabildiği son yıllarda yapılan çalışmalarda gösterilmiştir. 1,2 ROT oluşumundaki artış, antioksidan enzim düzeylerinde azalma ve/veya DNA onarım mekanizmalarındaki hatalar, oksidatif DNA hasarının artışına yol açmaktadır. Oksidatif DNA hasarının artışına bağlı olarak DNA'da tek ve çift sarmal kı-rıkları, abazik alanlar, baz modifikasyonları, deoksiriboz hasarı meydana gelebilmekte veya DNA ile protein arasında çapraz bağlanmalar olabilmektedir.…”

Section: Di̇abetes Melli̇tusunclassified

The Effects of Plant Extract Pycnogenol® on Diabetes Mellitus Therapy: Review

Bacanlı¹,

Anlar²,

Arı³

et al. 2017

Turkiye Klinikleri J Pharm Sci

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Ö ÖZ ZE ET T Metabolik bir bozukluk olan diabetes mellitus, insülin salgılanmasında mutlak veya göreceli eksiklikler ve/veya kronik hiperglisemiyle ilişkili insülin aktivitesindeki eksikliklerle karakterizedir. Özellikle gelişmekte olan ülkeler başta olmak üzere dünyada diyabet görülme sıklığı artmaktadır. Diyabet tedavisinde kullanılan ilaçlar yüksek maliyetli, sınırlı etkinlik ve tolerans ve/veya önemli istenmeyen etkilere sahiptir. Bu faktörlerin sonucu olarak hastalar bitkisel ilaçlar gibi alternatif tedavilere yönelmektedir. Bütün diyabet tiplerinde oksidatif stresin arttığını gösteren ça-lışmalar mevcuttur. Diyabette karşılaşılan oksidatif stresin oluşumunda; reaktif oksijen türleri (ROT) üretiminde artış ve/veya endojen antioksidanların bozulmuş aktivitesinin ayrı ayrı veya birlikte rol oynadığı bilinmektedir. Diyabette serbest radikal oluşumunun arttığı ve radikal bağlayıcı sistemlerde azalma olduğu ileri sürülerek, diyabet hastalarının antioksidan özellikli maddelere daha çok gereksinim duyabileceği savunulmaktadır. Bitkiler; fenolik, flavonoid ve tanenleri değişen miktarda içerebilmektedir. Birçok çalışma fitokimyasalların antioksidan özellikleri nedeni ile antidiyabetik etkileri üzerine yoğunlaşmaktadır. Piknogenol ® , Pinus maritima'dan elde edilen, serbest oksijen ve nitrojen radikallerini süpürme yeteneğine sahip standardize bir ekstredir. Piknogenol ® antioksidan özelliği nedeni ile kardiyovasküler hastalıklar, kanser, nörolojik bozukluklar ve diyabet gibi birçok kronik hastalığa karşı koruyucudur ve bu hastalıkların tedavisinde kullanılmakta-dır. Bu çalışmada, diyabet hakkında genel bilgiler, oksidatif stres ile diyabet arasındaki ilişki ve antioksidan kullanımının diyabetteki rolü ve önemli bir bitkisel ekstre olan Piknogenol ® 'ün diabetes mellitus tedavisine etkileri tartışılmıştır.A An na ah ht ta ar r K Ke el li im me el le er r: : Piknogenoller; proantosiyanidinler; diabetes mellitus A AB BS ST TR RA AC CT T Diabetes mellitus, a metabolic disorder, is characterized by absolute or relative deficiencies in insulin secretion and/or insulin action associated with chronic hyperglycemia. The prevalence of diabetes is increasing worldwide, especially developing countries. The drugs used in diabetes treatment have higher costs, limited efficacy, and tolerability and/or significant side effects. As a result of these factors, patients often have recourse to alternative forms of therapy such as herbal medicines. Some studies indicate that oxidative stress increased in all types of diabetes, it is known that production of reactive oxygen species (ROS) and/or impaired activity of endogenous antioxidants play role together or separately in formation of oxidative stress in diabetes. Production of free radicals was increased and radical binding systems were decreased in diabetes; due to these effects, diabetes patients need more antioxidants. Plants often contain various amounts of phenolics, flavonoids and tannins. Most of the studies are focused on the antidiabetic effects of these phytochemicals due to ...

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“…Uji Teknologi Pangan dan Hasil Pertanian, Fakultas Pertanian UGM, 2010). Antioksidan adalah inhibitor yang bekerja menghambat oksidasi dengan cara bereaksi dengan radikal bebas reaktif membentuk radikal bebas tak reaktif yang relatif stabil atau senyawa yang melindungi sel dari efek berbahaya radikal bebas oksigen reaktif (Laguerre et al, 2007;Matkowski et al, 2006;Rao et al, 2007;Montoro et al, 2005). Menurut (Kadam et al, 2008;Deng et al, 2007), antioksidan alami Vol.…”

Section: Pendahuluanunclassified

Aplikasi Mengkudu Sebagai Sumber Antioksidan Untuk Mengatasi Stress Ayam Broiler Di Daerah Tropis

Syahruddin¹,

Abbas²,

Purwati³

et al. 2012

JPI

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Antioxidant and cytotoxic activities of naturally occurring phenolic and related compounds: A comparative study

Cited by 128 publications

References 32 publications

Assessment of the in vitro cytotoxicity and in vivo anti-tumor activity of the alcoholic stem bark extract/fractions of Mimusops elengi Linn.

Assessment of the in vitro cytotoxicity and in vivo anti-tumor activity of the alcoholic stem bark extract/fractions of Mimusops elengi Linn.

The Effects of Plant Extract Pycnogenol® on Diabetes Mellitus Therapy: Review

Aplikasi Mengkudu Sebagai Sumber Antioksidan Untuk Mengatasi Stress Ayam Broiler Di Daerah Tropis

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