Antioxidant and cytoprotective effects of synthetic peptides identified from Kluyveromyces marxianus protein hydrolysate: Insight into the molecular mechanism

Mirdamadi, Saeed; Mirzaei, Mahta; Soleymanzadeh, Nazila; Safavi, Maliheh; Bakhtiari, Nuredin; Zandi, Mohammad

doi:10.1016/j.lwt.2021.111792

Cited by 19 publications

(13 citation statements)

References 39 publications

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“…The Val, Pro, and Phe residues were enriched at the N-terminal and C-terminal of AP-D10. This is consistent with the sequences in several antioxidant peptides, which were rich in Gly, Val, Pro, and Leu at the N-terminal region [ 42 ].…”

Section: Resultssupporting

confidence: 87%

Selection of Potential Probiotic Yeasts from Dry-Cured Xuanwei Ham and Identification of Yeast-Derived Antioxidant Peptides

et al. 2022

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The aim of this study was to select potential probiotic yeasts from dry-cured Xuanwei ham and investigate yeast-derived antioxidant peptides. The results showed that two strains (XHY69 and XHY79) were selected as potential probiotic yeasts and identified as Yamadazyma triangularis. The two yeasts showed tolerance under pH 2.5 and 1% bile salt, in addition to protease activity, auto-aggregation, antibacterial, and antioxidant activities. The peptide fraction (MW < 3 kDa) isolated from XHY69 fermentation broth, named XHY69AP, showed higher radical scavenging activities than glutathione at a concentration of 4.5 mg/mL (p < 0.05). The fraction (AP-D10) was purified from XHY69AP by gel filtration chromatography and reversed-phase high performance liquid chromatography, and then further identified by a UHPLC-LTQ-Orbitrap mass spectrometer. The molecular weight of all 55 purified sequences was distributed between 0.370 and 0.735 kDa. Among these seven novel peptides, Tyr-Pro-Leu-Pro (YPLP), Ala-Gly-Pro-Leu (AGPL), Gly-Pro-Phe-Pro (GPFP), and Ala-Pro-Gly-Gly-Phe (APGGF) were identified. All sequences were abundant in hydrophobic amino acids, especially proline residue. Among these novel peptides, YPLP possessed the highest ABTS scavenging rate (75.48%). The present work selects two new probiotic potential yeasts from dry-cured Xuanwei ham that are effective to yield novel antioxidant peptides.

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Section: Resultssupporting

confidence: 87%

Selection of Potential Probiotic Yeasts from Dry-Cured Xuanwei Ham and Identification of Yeast-Derived Antioxidant Peptides

et al. 2022

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“…In cellular assays, Cys containing peptides also exhibited a significant increase in cellular antioxidant enzyme activity. The keap1-Nrf2-ARE signaling pathway is the most important regulator of the cytoprotective response of cells to oxidative stress ( Mirdamadi, Mirzaei, Soleymanzadeh, Safavi, Bakhtiari, & Zandi, 2021 ). Antioxidant peptides exerted cellular protective effects via the keap1-Nrf2-ARE signaling pathway.…”

Section: Discussionmentioning

confidence: 99%

“…CAT, SOD, GSH-Px) to avoid oxidative damage in cells ( Huo et al, 2022 ). Mirdamadi et al indicated that the Cys in peptides could form hydrogen bonds with key amino acid residues in the binding site for Nrf2 peptide in the keap1 kelch domain, disrupted the keap1-Nrf2 interaction and promote antioxidant enzyme expression ( Mirdamadi, Mirzaei, Soleymanzadeh, Safavi, Bakhtiari, & Zandi, 2021 ). However, in this study, the peptide GCYVPKC, which contains two Cys residues, showed lower in vitro free radical scavenging activity and cellular antioxidant activity than CIGYDR, which contains only one Cys.…”

Section: Discussionmentioning

confidence: 99%

Abalone visceral peptides containing Cys and Tyr exhibit strong in vitro antioxidant activity and cytoprotective effects against oxidative damage

Liu

Yang

et al. 2023

Food Chemistry: X

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“…It was reported that peptides (NPYVPR, AVPYPQR, KVLPVPEK, and ARHPHPHLSFM) are identified in milk‐protected Caco‐2 cells against oxidative stress induced by H 2 O 2 by increasing the levels of both antioxidant molecules and antioxidant enzymes and reducing the production of ROS (Tonolo et al, 2018 ). In a similar study, Mirdamadi et al found that three peptides (VLSTSFCPK, VLSTSFYPK, and STSFPPK) obtained from Kluyveromyces marxianus protein hydrolyzate could activate the Keap1‐Nrf2 signaling pathway, which may lead to a decrease in lipid and protein oxidation and cell apoptosis and an increase in cell viability (Mirdamadi et al, 2021 ).…”

Section: Prefacementioning

confidence: 98%

Octopus‐derived antioxidant peptide protects against hydrogen peroxide‐induced oxidative stress in IEC‐6 cells

Peng

Cai

Pan

2022

Food Science & Nutrition

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This study aims to find antioxidant peptides from octopus protein hydrolyzates and verify the protective effects against H2O2‐induced oxidative stress in IEC‐6 cells. After the alcalase hydrolysate was ultrafiltrated, purified by Sephadex G‐25 gel fractionation and semipreparative reversed‐phase high‐performance liquid chromatography (RP‐HPLC), 16 peptides were identified, and chemically synthesized. In particular, the peptides AQNY, AMMLAW, FEGAW, GGAW, VDTVVCVW, and VVCLW showed better oxygen radical absorbance capacity (ORAC) and ABTS radical scavenging capacity. Among them, the smallest‐molecular‐weight peptide GGAW exhibited the best antioxidant activity. Furthermore, GGAW protected IEC‐6 cells from H2O2‐induced oxidative damage by significantly reducing the generation of reactive oxygen species (ROS), malondialdehyde (MDA), and lactate dehydrogenase (LDH), and increasing the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH‐PX), thereby improving cell viability. These results indicated that the peptide GGAW possessed the antioxidant capacity to prevent oxidative stress damage.

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Antioxidant and cytoprotective effects of synthetic peptides identified from Kluyveromyces marxianus protein hydrolysate: Insight into the molecular mechanism

Cited by 19 publications

References 39 publications

Selection of Potential Probiotic Yeasts from Dry-Cured Xuanwei Ham and Identification of Yeast-Derived Antioxidant Peptides

Selection of Potential Probiotic Yeasts from Dry-Cured Xuanwei Ham and Identification of Yeast-Derived Antioxidant Peptides

Abalone visceral peptides containing Cys and Tyr exhibit strong in vitro antioxidant activity and cytoprotective effects against oxidative damage

Octopus‐derived antioxidant peptide protects against hydrogen peroxide‐induced oxidative stress in IEC‐6 cells

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