Antioxidant and antityrosinase activity of mulberry (Morus alba L.) twigs and root bark

Chang, Lee-Wen; Juang, Lih-Jeng; Wang, Bor-Sen; Wang, Meiying; Tai, Huo-Mu; Hung, Wei-Jing; Chen, Yun‐Ju; Huang, Ming‐Hsing

doi:10.1016/j.fct.2010.11.045

Cited by 147 publications

(116 citation statements)

References 31 publications

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“…Arbutin, a structural analog of hydroquinone, can be metabolized into hydroquinone (Blaut et al, 2006), increasing the possibility of getting cancer. Thus, much research has identified tyrosinase inhibitors synthesized in the laboratory (Kubo et al, 2000;Shiino et al, 2001;Jun et al, 2007;Jirawattanapong et al, 2009;Delogu et al, 2010;Yi et al, 2010;Cha et al, 2011;Tajima et al, 2011;Song et al, 2012;Hamidian, 2013;Hamidian et al, 2013;Zhu et al, 2013) and extracted from plants (Piao et al, 2009;Sung et al, 2009;Chang et al, 2011;Liang et al, 2012;Zheng et al, 2012;Sarkhail et al, 2013). Some attention has been drawn to applying peptide sequences for tyrosinase inhibition; however, most of them are fragments isolated from known proteins and peptide-derived compounds.…”

Section: Introductionmentioning

confidence: 99%

Phage Display–Mediated Discovery of Novel Tyrosinase-Targeting Tetrapeptide Inhibitors Reveals the Significance of N-Terminal Preference of Cysteine Residues and Their Functional Sulfur Atom

et al. 2014

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Tyrosinase, a key copper-containing enzyme involved in melanin biosynthesis, is closely associated with hyperpigmentation disorders, cancer, and neurodegenerative diseases, and as such, it is an essential target in medicine and cosmetics. Known tyrosinase inhibitors possess adverse side effects, and there are no safety regulations; therefore, it is necessary to develop new inhibitors with fewer side effects and less toxicity. Peptides are exquisitely specific to their in vivo targets, with high potencies and relatively few off-target side effects. Thus, we systematically and comprehensively investigated the tyrosinase-inhibitory abilities of N-and C-terminal cysteine/tyrosine-containing tetrapeptides by constructing a phage-display random tetrapeptide library and conducting computational molecular docking studies on novel tyrosinase tetrapeptide inhibitors. We found that N-terminal cysteine-containing tetrapeptides exhibited the most potent tyrosinase-inhibitory abilities. The positional preference of cysteine residues at the N terminus in the tetrapeptides significantly contributed to their tyrosinase-inhibitory function. The sulfur atom in cysteine moieties of N-and C-terminal cysteine-containing tetrapeptides coordinated with copper ions, which then tightly blocked substrate-binding sites. N-and C-terminal tyrosinecontaining tetrapeptides functioned as competitive inhibitors against mushroom tyrosinase by using the phenol ring of tyrosine to stack with the imidazole ring of His263, thus competing for the substrate-binding site. The N-terminal cysteine-containing tetrapeptide CRVI exhibited the strongest tyrosinase-inhibitory potency (with an IC 50 of 2.7 6 0.5 mM), which was superior to those of the known tyrosinase inhibitors (arbutin and kojic acid) and outperformed kojic acid-tripeptides, mimosine-FFY, and shortsequence oligopeptides at inhibiting mushroom tyrosinase.

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Section: Introductionmentioning

confidence: 99%

Phage Display–Mediated Discovery of Novel Tyrosinase-Targeting Tetrapeptide Inhibitors Reveals the Significance of N-Terminal Preference of Cysteine Residues and Their Functional Sulfur Atom

et al. 2014

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“…Recent discoveries in the field of Morus alba L. phytochemicals revealed that Morus alba L. twig extracts contain polyphenols, which are major effective antioxidants. [26][27][28][29] Meanwhile, monomer polyphenols including oxyresveratrol, resveratrol [30,31] , mulberroside, rutin [32] , quercetin [33] , and morin, [34] which exist in Morus alba L. twig are more extensive, have high antioxidant activity, and are effective scavengers of free radicals. Six polyphenols, in particular, including rutin, quercetin, and morin, have been isolated and shown to possess high free radical scavenging activity [35] , and resveratrol and rutin can protect against DNA damage.…”

Section: Introductionmentioning

confidence: 99%

DNA protection activity of a hydroethanol extract and six polyphenol monomers fromMorus alba L. (mulberry) twig

Jia

et al. 2017

International Journal of Food Properties

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Morus alba L. (mulberry) is one of the most important medicine and food crops in China and other developing countries in Asia due to its large biomass (twigs) and useful by-products. Additionally, its twigs are an excellent green resource for bioactive compounds that are resistant to ultraviolet (UV) radiation and that can whiten skin and scavenge free radicals, and are of potential interest to the cosmetics and health food industries. In this study, we evaluated the ability of Morus alba L. twig hydroethanol extract and isolated compounds to protect DNA against UV damage. Agarose gel electrophoresis showed that a hydroethanol extract and six different polyphenol monomers (oxyresveratrol, resveratrol, mulberroside A, rutin, quercetin, and morin) present in the extract protected pUC19 plasmid DNA from UV damage. The optimal concentrations of polyphenols and Morus alba L. twig extract for DNA protection were 0.500 mg/mL and 1.00 mg/mL, respectively. These compounds may have potential as skin-care products to prevent UV-induced damage to the skin. Additionally, activity contribution degree assessment revealed other Morus alba L. substances possessing significant DNA protection activity that are worthy of further investigation. ARTICLE HISTORY

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“…In recent studies, isoquercitrin has been reported to possess anti-Mayaro virus, anti-inflammatory, anti-oxidative, anti-allergic, anti-hypertensive, anti-hyperglycemic, and saluretic/diuretic effects [15][16][17][18][19][20][21][22][23]. Although the antioxidative and neuroprotective effects of isoquercitrin have been established, whether isoquercitrin could exert an anti-apoptotic effect on endothelial cells is not clear.…”

Section: Introductionmentioning

confidence: 99%

“…Isoquercitrin, also known as quercetin-3-glucoside, is a glucose-bound derivative of quercetin that is commonly found in medicinal herbs, fruits, vegetables, and plant-derived foods and beverages [10][11][12][13][14]. In recent studies, isoquercitrin has been reported to possess anti-Mayaro virus, anti-inflammatory, anti-oxidative, anti-allergic, anti-hypertensive, anti-hyperglycemic, and saluretic/diuretic effects [15][16][17][18][19][20][21][22][23]. Although the antioxidative and neuroprotective effects of isoquercitrin have been established, whether isoquercitrin could exert an anti-apoptotic effect on endothelial cells is not clear.…”

Section: Molecules2016 21 360mentioning

confidence: 99%

Isoquercitrin Inhibits Hydrogen Peroxide-Induced Apoptosis of EA.hy926 Cells via the PI3K/Akt/GSK3β Signaling Pathway

Zhu

Wang

et al. 2016

Molecules

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Abstract:Oxidative stress plays a critical role in endothelial injury and the pathogenesis of diverse cardiovascular diseases, including atherosclerosis. Isoquercitrin (quercetin-3-glucoside), a flavonoid distributed widely in plants, exhibits many biological activities, including anti-allergic, anti-viral, anti-inflammatory, and anti-oxidative effects. In the present study, the inhibitory effect of isoquercitrin on H 2 O 2 -induced apoptosis of EA.hy926 cells was evaluated. MTT assays showed that isoquercitrin significantly inhibited H 2 O 2 -induced loss of viability in EA.hy926 cells. Hoechst33342/PI and Annexin V-FITC/PI fluorescent double staining indicated that isoquercitrin inhibited H 2 O 2 -induced apoptosis of EA.hy926 cells. Western blotting demonstrated that isoquercitrin prevented H 2 O 2 -induced increases in cleaved caspase-9 and cleaved caspase-3 expression, while increasing expression of anti-apoptotic protein Mcl-1. Additionally, isoquercitrin significantly increased the expression of p-Akt and p-GSK3β in a dose-dependent manner in EA.hy926 cells. LY294002, a PI3K/Akt inhibitor, inhibited isoquercitrin-induced GSK3β phosphorylation and increase of Mcl-1 expression, which indicated that regulation of isoquercitrin on Mcl-1 expression was likely related to the modulation of Akt activation. These results demonstrated that the anti-apoptotic effect of isoquercitrin on H 2 O 2 -induced EA.hy926 cells was likely associated with the regulation of isoquercitrin on Akt/GSK3β signaling pathway and that isoquercitrin could be used clinically to interfere with the progression of endothelial injury-associated cardiovascular disease.

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Antioxidant and antityrosinase activity of mulberry (Morus alba L.) twigs and root bark

Cited by 147 publications

References 31 publications

Phage Display–Mediated Discovery of Novel Tyrosinase-Targeting Tetrapeptide Inhibitors Reveals the Significance of N-Terminal Preference of Cysteine Residues and Their Functional Sulfur Atom

Phage Display–Mediated Discovery of Novel Tyrosinase-Targeting Tetrapeptide Inhibitors Reveals the Significance of N-Terminal Preference of Cysteine Residues and Their Functional Sulfur Atom

DNA protection activity of a hydroethanol extract and six polyphenol monomers fromMorus alba L. (mulberry) twig

Isoquercitrin Inhibits Hydrogen Peroxide-Induced Apoptosis of EA.hy926 Cells via the PI3K/Akt/GSK3β Signaling Pathway

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