Antioxidant and Antipruritic Activities of Ethyl Acetate Fraction from Diospyros lotus Leaves

Abstract

Diospyros lotus has been cultivated for its edible fruits, which are considered to have medicinal importance. The aim of this study was to evaluate the antioxidant and antipruritic activities of water-soluble, methanol extract, and ethyl acetate (EA) fractions from D. lotus leaves. The EA fraction showed the lowest IC50 vale (DPPH: 5.3 μg/mL, ABTS: 53.8 μg/mL). Therefore, we further investigated anti-inflammatory and antipruritic effects of the EA fraction. TNF-α production increased by PMA plus A23187 treatment was significantly inhibited by the EA fraction in a dose-dependent manner. The EA fraction also inhibited histamine release from rat peritoneal mast cells stimulated by compound 48/80, which promotes histamine release. Furthermore, EA fraction had inhibitory effects on scratching behavior induced by compound 48/80 in Balb/c mice. These results suggest that the EA fraction from D. lotus leaves has potential as ameliorative agent against oxidative stress and pruritus-related disease.

Nitrite Scavenging and Inhibition of N-Nitrosamines Formation by Phenolic Extracts From Diospyros lotus L. Leaves and Active Ingredients

“…Recent studies have found that the leaves have various activities, such as liver protection, 22 antiultraviolet damage, 23 and antipruritic. 24 Phenolic compounds isolated from the fruits and leaves of D. lotus, including gallic acid, methyl gallate, ellagic acid, kaempferol, quercetin, myricetin (Myt), myricetin 3-O-β-glucuronide, and myricitrin (Mytr), showed antioxidant activity. 17,25 However, there are no reports about nitrite scavenging and inhibition of NAs formation by D. lotus leaves and their active ingredients.…”