Antioxidant and Antimutagenic (Anticlastogenic) Activity of Alcoholic Extract of Bauhinia variegata (Linn.) Root

Abstract

Aims: Antioxidant and Antimutagenic (Anticlastogenic) activity of alcoholic extract of Bauhinia variegata (Linn.) root.
 Place: C. U. Shah College of Pharmacy and Research, Wadhwan, Surendranagar, Gujarat, India.
 Methodology: Shade dried Bauhinia variegata (Linn.) root, extraction was carryout by isolation extract were subjected to primary and secondary Phytochemical investigation. Then In-vitro antioxidant properties were estimated by reducing power and nitric oxide free radical scavenging method. Based on Phytochemical constituent and antioxidant properties In-vivo Antimutagenic (Anticlastogenic) activity was performed.
 Results: Preliminary phytochemical investigation revealed the presence of carbohydrates, free amino acids, and secondary metabolites like tannins, phenolic compounds and flavonoids, then polyphenol estimation found ALBV contains 86.38% phenolic compounds. In antioxidant properties determination IC50 respectively found 55.27±2.57 µg/ml, 125.52±8.15 µg/ml against of Ascorbic acid and Curcumin. Then In-vivo Antimutagenic (Anticlastogenic) activity ALBV shows significant reeducation in % MNPCE, % MNNCE and P/N ratio at 24 h, 48 h and 72 h against the cyclophosphamide-induced mutagenicity.
 Conclusion: Therefore, from the present study, it is concluded that alcoholic extract of Bauhinia variegata root (ALBV) can prove to be a very good antioxidant and effective chemopreventive against cyclophosphamide-induced mutagenesis.

Phytochemical and antioxidant activities of methanolic extract of Lawsonia inermis L. Bark

“…Total polyphenol content (TPC) was determined by applying the spectrophotometry method, using gallic acid as standard, according to the method described by the International Organization for Standardization (ISO) [21]. Briefly, 1 mL of the diluted sample extract was transferred in duplicate to separate tubes containing 5 mL of a 1/10 dilution of Folin-Ciocalteu's reagent in water [22]. Subsequently, 4 mL of a sodium carbonate solution (7.5% w/v) was added.…”