Antioxidant and antimicrobial properties of squalene from Symphytum officinale and chlorogenic acid from trifolium pratense

Dmitrieva, Anastasia; Vesnina, Anna; Dyshlyuk, Lyubov

doi:10.1063/5.0104513

Cited by 4 publications

(2 citation statements)

References 26 publications

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“…Microbial growth was also observed on some of the emulsions after the tenth day of storage at 25 • C. Microbial growth was not observed for samples containing α-tocopherol and squalene that were stored at ambient temperature and for all samples that were stored at 4 • C. It has been previously reported that microbial growth started for SPI emulsions after 6 days of storage at ambient temperature and after 9 or 10 days for PPI emulsions [74,75]. Furthermore, squalene as well as α-tocopherol have been found to act against microorganisms, which explains the resistance of the samples containing them against microbial growth [76,77].…”

Section: Stability Assessment Against Creaming and Coalescence Of The...mentioning

confidence: 82%

Pea and Soy Protein Stabilized Emulsions: Formulation, Structure, and Stability Studies

Galani

Laurichesse

et al. 2023

Colloids and Interfaces

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During the last decades, there has been a huge consumer concern about animal proteins that has led to their replacement with plant proteins. Most of those proteins exhibit emulsifying properties; thus, the food industry begins their extensive use in various food matrices. In the present study, pea and soy protein isolates (PPI and SPI) were tested as potential candidates for stabilizing food emulsions to encapsulate α-tocopherol and squalene. More specifically, PPI and SPI particles were formulated using the pH modification method. Following, emulsions were prepared using high-shear homogenization and were observed at both a microscopic and macroscopic level. Furthermore, the adsorption of the proteins was measured using the bicinchoninic acid protein assay. The emulsions’ droplet size as well as their antioxidant capacity were also evaluated. It was found that the droplet diameter of the SPI-based emulsions was 60.0 μm, while the PPI ones had a relatively smaller diameter of approximately 57.9 μm. In the presence of the bioactives, both emulsions showed scavenging activity of the 2,20-Azinobis-(3-ethylbenzothiazoline-6-sulphonate) radical cation (ABTS·+) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals, with the ones loaded with α-tocopherol having the greatest antioxidant capacity. Overall, the proposed systems are very good candidates in different food matrices, with applications ranging from vegan milks and soups to meat alternative products.

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Section: Stability Assessment Against Creaming and Coalescence Of The...mentioning

confidence: 82%

Pea and Soy Protein Stabilized Emulsions: Formulation, Structure, and Stability Studies

Galani

Laurichesse

et al. 2023

Colloids and Interfaces

View full text Add to dashboard Cite

show abstract

“…Adhering to a proper diet (consumption of fruits, vegetables, and various antioxidant-rich, anti-inflammatory, and other bioactive substances (BAS)) contributes to improving human health and healthy aging. However, due to economic, climatic, geographical, and other limitations, the systematic consumption of certain products is difficult [1,2]. Therefore, the development of biologically active supplements (BAS) is relevant.…”

Section: Introductionmentioning

confidence: 99%

Study of the hepatotoxicity of trans-coric acid isolated fromScutellaria baicalensisinin vivoexperiments

Mikhailovna,

Dmitrievna,

Sergeevna

et al. 2024

Preprint

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Biologically active supplements (BAS) based on plant material, possessing a wide range of beneficial properties (antioxidant, anti-inflammatory, antimicrobial, etc.), are becoming increasingly popular worldwide. Such supplements are readily available and can be used without consulting a healthcare professional. Consequently, the extent of their use is underestimated by doctors. Plant hepatotoxicity is not uncommon, but the frequency and manifestation are not sufficiently characterized. This study is dedicated to investigating the hepatotoxicity of trans-coric acid isolated from the root culture of Scutellaria baicalensis in in vivo experiments. This work aims to evaluate the hepatotoxicity of trans-coric acid on male rats at doses of 50.0 and 100.0 mg/kg. During the experiment, it was found that trans-coric acid, isolated from the root culture extract of Scutellaria baicalensis, does not induce hepatotoxicity in the presented doses in model animals with 14-day intragastric administration. Thus, this study demonstrates that trans-coric acid can be safely used as a component of BAS for the prevention of chronic diseases, thereby promoting healthy aging

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Chlorogenic Acid and Biohanin A from Trifolium pratense L. Callus Culture Extract: Functional Activity In Vivo

Milentyeva,

Vesnina,

Fedorova

et al. 2023

Food Processing: Techniques and Technology

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Polyphenols are potential neuroprotectors that increase lifespan and slow down aging. Red clover (Trifolium pratense L.) is a promising source of biologically active substances. Its extracts contain biochanin A and chlorogenic acid. This research used Caenorhabditis elegans to study the effect of polyphenols extracted from red clover callus cultures on SOD-3 and HSP-16.2 genes, as well as their anti-amyloid potential. The chlorogenic acid and biochanin A (200, 100, 50, and 10 µM) with a purity of 95% were isolated from callus extracts of T. pratense L. The effect of polyphenols on SOD-3 and HSP-16.2 was assessed after 5 and 2 h of heat stress (35°C), respectively, using C. elegans N2 Bristol as model organism. The neuroprotective potential was measured by counting paralyzed nematodes after 18, 40, and 62 h of incubation. The research established a dose-dependent effect between the concentration of biologically active substances and the percentage of paralyzed nematodes after 18 h of cultivation. The lowest paralysis phenotype count occurred at a concentration of 200 μM. The activity of 200 μM biochnin A was 1.18 times as high as that of a 200 μM chlorogenic acid solution. Biochanin A solutions increased SOD-3 expression by 3.7 times, compared to the control. The biologically active substances exhibited relative neuroprotective activity and affected the expression of antioxidant defense gene in C. elegans.

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Antioxidant and antimicrobial properties of squalene from Symphytum officinale and chlorogenic acid from trifolium pratense

Cited by 4 publications

References 26 publications

Pea and Soy Protein Stabilized Emulsions: Formulation, Structure, and Stability Studies

Pea and Soy Protein Stabilized Emulsions: Formulation, Structure, and Stability Studies

Study of the hepatotoxicity of trans-coric acid isolated fromScutellaria baicalensisinin vivoexperiments

Chlorogenic Acid and Biohanin A from Trifolium pratense L. Callus Culture Extract: Functional Activity In Vivo

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