Antioxidant and Antimicrobial Effects of Solvent Fractions from Smilax china L. Leaves

Abstract

To utilize Smilax china L. leaves as a natural preservatives, solvent fractions from crude methanol extract were prepared and investigated their antioxidant and antimicrobial activities against Staphylococcus aureus. Antioxidant activities were examined by 1,1-diphenyl-1-picrylhydrazyl radical scavenging and ferric ion reducing antioxidant power assays. Ethyl acetate fraction from Smilax china L. leaves exhibited the strongest antioxidant and antimicrobial activities among the fractions (P<0.05), as well as the highest total phenolic and total flavonoid contents. Caffeic acid, ferulic acid, quercetin, and kaempferol contents in the ethyl acetate fraction from Smilax china L. leaves were determined by TLC and HPLC. In aqueous phase, as well as the n-butanol and n-hexane fractions, quercetin, ferulic acid, and kaempferol were detected in small amounts. Ferulic acid, which showed the highest content, is the major active compound from Smilax china L. leaves.

Anti-adipogenic activity of Smilax sieboldii extracts in 3T3-L1 adipocytes

“…청미래덩굴은 잎이 두껍고 윤기가 나 며 열매가 붉지만, 청가시덩굴은 잎이 보다 얇고 열매는 검은 빛을 띄는 특징이 있다. 청미래덩굴 추출물은 다양한 생리활성 성분을 통한 향균 및 항산화력에 대한 효능이 알려져 있고 항 염증, 항비만, 항당뇨 등 여러 연구 결과가 보고되어 있다 [10][11][12]. 그러나 청미래덩굴속에 속하는 청가시덩굴에 대한 연구는 통증을 없애는 효능과 독성에 관한 보고가 되었을 뿐 아직까지 생리활성에 관한 연구가 미흡한 실정이다 [13,14].…”