Antioxidant and Antimicrobial Activity of Zostera marina L. Extract

Abstract

Methanol crude extract of the sea grass Zostera marina L. and organic solvent fractions (n-hexane, chloroform, ethyl acetate, n-butanol, and water) were screened for antioxidant activity (total phenolic contents, DPPH scavenging activity, and reducing power) and antimicrobial activity against three human skin pathogens, two bacteria and a yeast; Staphylococcus aureus, Staphylococcus epidermidis, and Candida albicans. Total phenolic contents and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity were highest in the ethyl acetate fraction with 968.50 µg gallic acid equivalent per milligram of extract, and ca. 95% scavenging activity on the DPPH radicals at 10 mg ml -1 . In antimicrobial activity tests, MICs (Minimum Inhibitory Concentration) of each Zostera marina extract partitioned ranged from 1mg to 8 mg ml -1 (extract/ 10% DMSO) against all three human skin pathogens. The MICs of the ethyl acetate and n-butanol fractions were the same with 1 mg ml -1 against S. aureus and C. albicans. The ethyl acetate fraction of Z. marina does protect against free radicals and may be used to inhibit the growth of human skin pathogens.

A comparative evaluation of biological activities and bioactive compounds of the seagrasses Zostera marina and Zostera noltei from southern Portugal

“…The proportion of n 2 3 PUFA detected in Z. marina and Z. noltei was approximately 40% of the total FAs; therefore, taking into account their FAs profile, both seagrasses can be considered to be a promising source of n 2 3 PUFA with valuable nutritional, pharmaceutical and biomedical applications. Z. marina had the highest radical scavenging activity (RSA) ( Table 1), which was lower than the RSA value previously reported for the ethyl acetate fraction of a crude methanol extract for the same species collected in Korea (Choi et al 2009). Z. marina also had a good capacity to chelate copper, although not iron (Table 1).…”

“…Taylor and Francis shall not be liable for any losses, actions, claims, proceedings, demands, costs, Alexandrium catenella, a dinoflagellate responsible for the occurrence of harmful algal blooms (Laabir et al 2013). Z. marina leaf extracts have antioxidant and antibiotic activities (Kim et al, 2004;Kolenchenko et al 2005;Choi et al 2009), while organic extracts of Z. noltei collected in the Central Mediterranean area had strong antifungal activity against Candida albicans and Aspergillus niger (Ballesteros et al 1992). Some bioactive compounds have been isolated from Z. marina, such as apigenin-7-O-b-D-glucoside and chrysoeriol (Kim et al 2004).…”

Ecosystem Services

“…No medicament is known from eelgrass but since intact leaves decay very slowly and are widely selected and used as roof materials, it may be a good resource for screening natural antibiotics and some studies have shown effective compounds with nematicidal and antibacterial activity from eelgrass extracts (Harrison 1982, Choi et al 2009, Liu et al 2010, Newmaster et al 2011, Wang et al 2012).…”

Hubungan Biomassa Epifit Dengan Aktivitas Antioksidan Lamun Di Perairan Pulau Pramuka, Kepulauan Seribu, Dki Jakarta

“…Lamun mampu menghasilkan metabolit sekunder (Choi et al 2009;Regalado et al 2012). Metabolit sekunder yang umumnya diproduksi oleh organisme berperan untuk pertahanan diri dari lingkungan maupun dari serangan organisme lain termasuk organisme penempel/epifit, dan mencegah adanya infeksi dari patogen (Murniasih 2003;Marhaeni et al 2010).…”