Antioxidant and antimicrobial activities of Onosma argentatum and Rubia peregrina

Ozgen, Ufuk; Houghton, Peter J.; Ogundipe, Y.; Coşkun, Maksut

doi:10.1016/s0367-326x(03)00161-8

Cited by 50 publications

(26 citation statements)

References 9 publications

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“…Our results are consistent with a study on the antibacterial effect of R. peregrina, which showed that the ethyl acetate and chloroform fractions of the extract are effective against S. aureus and Escherichia coli, respectively [57]. The anti-adhesion effects of R. peregrina and C. cirrhosa extracts were assessed and the results are shown in Figure 6.…”

Section: S Aureus Adhesion Test To Tetrahymena Pyriformis Cellssupporting

confidence: 91%

The Ciliate Protist Tetrahymena pyriformis as a Cellular Adhesion Model for the Pathogenic Bacterium Staphylococcus aureus

Khalfi¹,

Benlahfid²,

Jarmouni³

et al. 2017

ABB

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Staphylococcus aureus is one of the main pathogenic agents responsible for nosocomial and community-acquired bacterial infections. The pathogenicity of this Gram-positive bacterium is ensured by its different adhesion factors.Collagen and the extracellular glycoprotein adhesin are among the Staphylococcus most important virulence factors. It has been shown that most of the S. aureus strains carry the ica operon, responsible for biofilm production. However, the coexpression of the icaA and the icaD genes is necessary for complete biofilm synthesis. The aim of our study was to study a collection of 15 clinical strains of S. aureus from different sources for the presence of cna and icaD genes coding intercellular adhesion proteins. We also intended to estimate the strains' ability to form biofilms by the red Cong method and to test the adhesion ability of S. aureus to the ciliated protist Tetrahymena pyriformis, which we used as a novel cellular adhesion model. Finally, we checked the adhesion's inhibition capacity of some plants extracts. The molecular detection of adhesion genes revealed that 80% of strains are cna positive, and 73% are icaD positive. Qualitative biofilm production of S. aureus revealed that 66.6% of strains were slime producers. The adhesion test revealed that 20% of strains are strongly adhering to T. pyriformis and that the Clematis cirrhosa extract has an anti-adhering effect of S. aureus to the ciliate T. pyriformis.

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Section: S Aureus Adhesion Test To Tetrahymena Pyriformis Cellssupporting

confidence: 91%

The Ciliate Protist Tetrahymena pyriformis as a Cellular Adhesion Model for the Pathogenic Bacterium Staphylococcus aureus

Khalfi¹,

Benlahfid²,

Jarmouni³

et al. 2017

ABB

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“…peregrina is a perennial shrub of the family Rubiaceae producing fleshy and black berries. Its underground parts (roots and rhizomes) have antioxidant and antimicrobial activities (Ozgen, Houghton, Ogundipe, & Coskun, 2003). This plant was utilized for dyeing since it contains anthraquinone dyes (Usai, 2004).…”

Section: Introductionmentioning

confidence: 99%

Identification and quantification of anthocyanins in the berries of Pistacia lentiscus L., Phillyrea latifolia L. and Rubia peregrina L.

Longo

Scardino

Vasapollo

2007

Innovative Food Science & Emerging Technologies

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“…A warm solvent can favour the passage of the analytes from the vegetable matrix to solvent, increasing the concentration of analytes in the final extract and favouring the Soxhlet method with respect to other systems. This is the first work in which three different extraction techniques and various solvents were compared for the analysis of Onosma species; in other literature works on similar topics only one extraction method has been reported [23,24]. The most efficient extraction method (Soxhlet) and condition (ethyl acetate for 6 h) from the above study were selected to quantitate, for the first time, the naphthoquinones alkannin/shikonin mixture in six populations of O. echioides growing in central Italy.…”

Section: Comparison Of Extraction Methods Efficiencymentioning

confidence: 99%

“…In literature are reported some extraction methods of alkannin/shikonin and other naphthoquinones from Onosma species: shikonin and derivatives using the sonication method [23], shikonin derivatives using solvent reflux [17,24], alkannin derivatives by manual extraction at room temperature [25]. However, to our knowledge, an accurate investigation of the most efficient extraction method of alkannin/shikonin mixture from genus Onosma has never been reported.…”

Section: Introductionmentioning

confidence: 99%

Alkannin/shikonin mixture from roots of Onosma echioides (L.) L.: Extraction method study and quantification

Sagratini¹,

Cristalli²,

Giardinà³

et al. 2008

J of Separation Science

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This work reports the extraction procedures of alkannin/shikonin mixture from roots of six populations of Onosma echioides, by means of three extraction techniques: Soxhlet extraction, maceration and rapid solid-liquid dynamic extraction (RSLDE). Five solvents with different polarity (hexane, petroleum ether, chloroform, ethyl acetate, methanol) were also studied. Analysis of the extracts was performed by an HPLC-DAD (diode array detector) system. The most efficient extraction technique was Soxhlet procedure using ethyl acetate for 6 h. Studied samples of O. echioides showed an alkannin/shikonin content in the range of 0.02-0.24 mg/kg. Other naphthoquinone derivatives (deoxyalkannin/deoxyshikonin and 5,8-dihydroxy-2-(4-methyl-6-oxo-5,6-dihydro-2H-pyran-2-yl)-[1,4]naphthoquinone and arnebin-6) were found for the first time in O. echioides and characterized in the extracts using HPLC-MS apparatus equipped with an ESI ionization source.

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Antioxidant and antimicrobial activities of Onosma argentatum and Rubia peregrina

Cited by 50 publications

References 9 publications

The Ciliate Protist <i>Tetrahymena pyriformis</i> as a Cellular Adhesion Model for the Pathogenic Bacterium <i>Staphylococcus aureus</i>

The Ciliate Protist <i>Tetrahymena pyriformis</i> as a Cellular Adhesion Model for the Pathogenic Bacterium <i>Staphylococcus aureus</i>

Identification and quantification of anthocyanins in the berries of Pistacia lentiscus L., Phillyrea latifolia L. and Rubia peregrina L.

Alkannin/shikonin mixture from roots of Onosma echioides (L.) L.: Extraction method study and quantification

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Antioxidant and antimicrobial activities of Onosma argentatum and Rubia peregrina

Cited by 50 publications

References 9 publications

The Ciliate Protist &lt;i&gt;Tetrahymena pyriformis&lt;/i&gt; as a Cellular Adhesion Model for the Pathogenic Bacterium &lt;i&gt;Staphylococcus aureus&lt;/i&gt;

The Ciliate Protist &lt;i&gt;Tetrahymena pyriformis&lt;/i&gt; as a Cellular Adhesion Model for the Pathogenic Bacterium &lt;i&gt;Staphylococcus aureus&lt;/i&gt;

Identification and quantification of anthocyanins in the berries of Pistacia lentiscus L., Phillyrea latifolia L. and Rubia peregrina L.

Alkannin/shikonin mixture from roots of Onosma echioides (L.) L.: Extraction method study and quantification

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The Ciliate Protist <i>Tetrahymena pyriformis</i> as a Cellular Adhesion Model for the Pathogenic Bacterium <i>Staphylococcus aureus</i>

The Ciliate Protist <i>Tetrahymena pyriformis</i> as a Cellular Adhesion Model for the Pathogenic Bacterium <i>Staphylococcus aureus</i>