Antioxidant and Antiglycation Activity of Rhizosphere and Endophytic Actinobacteria of Xylocarpus granatum

Abstract

Utilization of endophytic bacteria such as actinobacteria is one of the alternatives to obtain the bioactive compounds similar to the host plant. This study aimed to identify compounds produced by rhizosphere and endophytic actinobacteria isolated from Xylocarpus granatum for their antioxidant and antiglycation activity. Actinobacterial culture supernatant was extracted using ethyl acetate with a ratio of 1:1 (v/v). Antioxidant activity was performed using 2,2-diphenyl-1-picrylhydrazyl (DPPH). Whole TLC-bioautography and phytochemical tests were used to predict the group of active compounds. Antiglycation activity was identified based on the ability to inhibit the formation of advanced glycation end products (AGEs). Fifteen actinobacterial isolates had been isolated from rhizosphere (ten isolates), fruit (two isolates), and leaf (two isolates) of X. granatum. All isolates had been tested for their antioxidant and antiglycation activity. Isolate XR2 had the highest antioxidant activity with IC50 value of 1719.26 mg/L. Meanwhile, the highest antiglycation activity was obtained from isolate XR8 with IC50 value of 327.62 mg/L. This study informs that actinobacteria also live in the fruit, leaves and rhizosphere of X. granatum. The existence of rhizosphere and endophytic actinobacteria from X. granatum and their antioxidant and antiglycation activities contributes to the understanding of their diversity and potency as an antioxidant and antiglycation agent.

Application of Box-Behnken Design for the Extraction of Padina australis

“…Determination of Antiglycation Activity Antiglycation activity was analyzed by method that described previously (Ariansyah, Batubara, Lestari, & Egra, 2019). The extract was dissolved in distilled water and made to a concentration of 1.0 mg/mL.…”

Identification, Characterization and Antioxidant Activity of Yellowish-Orange Pigments Actinobacteria

“…In recent, there have been several studies of antioxidant activity in actinobacteria in Indonesia, including the antioxidant activity of endophytic actinobacteria in mangosteen peel (Larasati et al 2020), Xylocarpus granatum (Ariansyah et al 2019;Ratte et al 2022), Eleutherine palmifolia (Shabira et al 2022). In addition, the study of pigmented bacteria has also been reported, including bacteria found in seaweed Kappahycus alvarezii (Arlita et al 2013), mangrove sediment bacteria (Riyanti et al 2019), and marine bacteria (Abubakar et al 2022).…”