Antioxidant and Antiglycation Activities of Syzygium paniculatum Gaertn and Inhibition of Digestive Enzymes Relevant to Type 2 Diabetes Mellitus

Kim, Sangseo; Semple, Susan J.; Simpson, Bradley S.; Deo, Permal

doi:10.1007/s11130-020-00858-4

Cited by 15 publications

(9 citation statements)

References 32 publications

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“…However, no studies have reported the role of CXCL-13 in promoting the immigration of mesenchymal stem cells into islet B cells. Previous studies have also shown that the protein kinase (AKT) signaling pathway is one of the major signaling pathways that protect the function and activity of islet B cells [16], and it plays an important role in the function and survival of islet cells. However, no studies have reported whether mesenchymal stem cells can protect islet injury in high-glucose environment through AKT signaling pathway.…”

Section: Introductionmentioning

confidence: 99%

hMSCs Migrate under the Chemotaxis of CXCL-13 and Enhance Islet B Cell Activity through p-AKT Signaling Pathway in High-Glucose Environment

Bian

Jiang

et al. 2022

Journal of Healthcare Engineering

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As a common clinical chronic disease, the incidence of diabetes is increasing year by year. According to the latest statistics from the International Diabetes Federation, as of 2019, the global prevalence of diabetes has reached 8.3%. This study aims to investigate the effect of CXCL-13 on the migration ability of human mesenchymal stem cells (hMSCs) and to clarify the specific molecular mechanism of the protective effect of hMSCs on islet B cells. The hMSCs were cultured in high-glucose environment, and the effect of CXCL-13 on the migration ability of hMSCs was determined by Transwell experiment. After coculture of hMSCs and islet B cells, the activity of cells was detected by CCK8 assay, the expression of Ki-67 in cells was detected by RT-PCR, and the expression of P53 was detected by Western blot to investigate the effect of hMSCs on the proliferation and apoptosis of islet B cells. The effect of hMSCs on the function of islet B cells was determined by glucose stimulated insulin secretion experiment. Transwell experiment results showed that CXCL-13 could promote the migration of hMSCs to islet B cells in high-glucose environment. The results of CCK-8 showed that the cell activity in the coculture group was significantly higher than that of the other groups, and RT-PCR showed that the expression of Ki-67 was significantly increased in the coculture group of hMSCs and islet B cells. The results of Western blot showed that the expression of P53 was significantly decreased in the coculture group, and the glucose stimulated insulin secretion test showed that insulin secretion was significantly increased. It was found that after the inhibition of ATK, cell activity was significantly reduced, and apoptosis was significantly increased. Meanwhile, the expression of Ki-67 was inhibited, the expression of P-53 was significantly increased, and insulin secretion was significantly reduced. To sum up, in a high-glucose environment, CXCL-13 effectively promoted the migration of hMSCs, and hMSCs protected the activity and function of islet B cells through Akt signaling pathway.

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Section: Introductionmentioning

confidence: 99%

hMSCs Migrate under the Chemotaxis of CXCL-13 and Enhance Islet B Cell Activity through p-AKT Signaling Pathway in High-Glucose Environment

Bian

Jiang

et al. 2022

Journal of Healthcare Engineering

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“…Phytochemicals and antioxidant potential of food extracts have been previously reported for its antiglycation activity which are attributed to the multiple stage targets in the AGE pathway (Li et al ., 2014; Wang et al ., 2016; Kim et al ., 2020). In this study, total TPC, TFC and FRAP levels were measured in H. scabra extracts and correlated with AGE biomarkers.…”

Section: Resultsmentioning

confidence: 99%

“…A glucose‐BSA glycation model was prepared as previously described (Kim et al ., 2020; Deo et al ., 2020a). Appropriate controls (BSA only), negative controls (AGE‐BSA) and positive control (AGE‐BSA‐AG) were also prepared.…”

Section: Methodsmentioning

confidence: 99%

Holothuria scabra Jaegar 1833 (Sandfish) extracts and collagens modulate protein‐bound N^ε‐carboxymethyllysine, N^ε‐carboxyethyllysine and methylglyoxal‐derived hydroimidazolone‐1 levels

Wong

Jaunay

Lau

et al. 2023

Int J of Food Sci Tech

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Summary In this study, sea cucumber (Holothuria scabra; Jaegar 1833) extracts and collagens were evaluated for inhibitory properties of protein‐bound advanced glycation end products (AGEs). Processed dried sea cucumber with salt extract showed a significant lower IC50 value for fluorescent AGEs (9.19 ± 7.68 μg mL−1, P < 0.05) and fructosamine (503.47 ± 46.37 μg mL−1, P < 0.05), respectively. Processed dried with and without salt extracts significantly reduced the Nε‐carboxymethyllysine (CML) and methylglyoxal‐derived hydroimidazolone‐1 (MG‐H1) levels tested at 250 μg mL−1. Smoked dried and fresh‐dried extracts significantly reduced the Nε‐carboxyethyllysine (CEL) levels tested at 250 μg mL−1. Pepsin‐solubilised collagen and the crude collagen fibrils significantly reduced CML and MG‐H1 levels whereas CEL levels were unchanged. Pearson's correlation analysis showed that protein‐bound AGE and CML inhibition significantly correlated with the total phenolic and antioxidant activities, respectively. This study provides a strong rationale for further investigation aimed at identifying the active compounds responsible for the observed effects on biomarkers relevant to diabetes.

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“…Additionally, inhibition of AGE formation is another approach in delaying or preventing the onset of diabetic complications [ 48 ]. Many studies conducted on fruits indicated that phenolic compounds were the functional components that played a major role in their hypoglycemic effect [ 7 , 8 , 9 , 12 , 27 , 31 , 49 , 50 , 51 , 52 ]. Thus, in order to verify this hypothesis in our research, the V. opulus fruit samples varying in terms of degree of purification of phenolic compounds and thus in the presence of other non-phenolic components were used.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, diet, weight control and exercise are essential and effective means of improving glucose homeostasis. Some medicinal plants, fruits, and vegetables have been reported in the literature as having been used to control diabetes [ 4 , 5 , 6 , 7 , 8 , 9 ]. In addition, the antidiabetic agents from the natural resources can be an alternative to hypoglycemic drugs that are not always satisfactory and show side effects [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Glycoside Hydrolases and Non-Enzymatic Glycation Inhibitory Potential of Viburnum opulus L. Fruit—In Vitro Studies

2021

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Phytochemicals of various origins are of great interest for their antidiabetic potential. In the present study, the inhibitory effects against carbohydrate digestive enzymes and non-enzymatic glycation, antioxidant capacity, and phenolic compounds composition of Viburnum opulus L. fruits have been studied. Crude extract (CE), purified extract (PE), and ethyl acetate (PEAF) and water (PEWF) fractions of PE were used in enzymatic assays to evaluate their inhibitory potential against α-amylase with potato and rice starch as substrate, α-glucosidase using maltose and sucrose as substrate, the antioxidant capacity (ABTS, ORAC and FRAP assays), antiglycation (BSA-fructose and BSA-glucose model) properties. Among four tested samples, PEAF not only had the highest content of total phenolics, but also possessed the strongest α-glucosidase inhibition, antiglycation and antioxidant activities. UPLC analysis revealed that this fraction contained mainly chlorogenic acid, proanthocyanidin oligomers and flavalignans. Contrary, the anti-amylase activity of V. opulus fruits probably occurs due to the presence of proanthocyanidin polymers and chlorogenic acids, especially dicaffeoylquinic acids present in PEWF. All V. opulus samples have an uncompetitive and mixed type inhibition against α-amylase and α-glucosidase, respectively. Considering strong anti-glucosidase, antioxidant and antiglycation activities, V. opulus fruits may find promising applications in nutraceuticals and functional foods with antidiabetic activity.

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Antioxidant and Antiglycation Activities of Syzygium paniculatum Gaertn and Inhibition of Digestive Enzymes Relevant to Type 2 Diabetes Mellitus

Cited by 15 publications

References 32 publications

hMSCs Migrate under the Chemotaxis of CXCL-13 and Enhance Islet B Cell Activity through p-AKT Signaling Pathway in High-Glucose Environment

hMSCs Migrate under the Chemotaxis of CXCL-13 and Enhance Islet B Cell Activity through p-AKT Signaling Pathway in High-Glucose Environment

Holothuria scabra Jaegar 1833 (Sandfish) extracts and collagens modulate protein‐bound N^ε‐carboxymethyllysine, N^ε‐carboxyethyllysine and methylglyoxal‐derived hydroimidazolone‐1 levels

Glycoside Hydrolases and Non-Enzymatic Glycation Inhibitory Potential of Viburnum opulus L. Fruit—In Vitro Studies

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Antioxidant and Antiglycation Activities of Syzygium paniculatum Gaertn and Inhibition of Digestive Enzymes Relevant to Type 2 Diabetes Mellitus

Cited by 15 publications

References 32 publications

hMSCs Migrate under the Chemotaxis of CXCL-13 and Enhance Islet B Cell Activity through p-AKT Signaling Pathway in High-Glucose Environment

hMSCs Migrate under the Chemotaxis of CXCL-13 and Enhance Islet B Cell Activity through p-AKT Signaling Pathway in High-Glucose Environment

Holothuria scabra Jaegar 1833 (Sandfish) extracts and collagens modulate protein‐bound Nε‐carboxymethyllysine, Nε‐carboxyethyllysine and methylglyoxal‐derived hydroimidazolone‐1 levels

Glycoside Hydrolases and Non-Enzymatic Glycation Inhibitory Potential of Viburnum opulus L. Fruit—In Vitro Studies

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Product

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Holothuria scabra Jaegar 1833 (Sandfish) extracts and collagens modulate protein‐bound N^ε‐carboxymethyllysine, N^ε‐carboxyethyllysine and methylglyoxal‐derived hydroimidazolone‐1 levels