Antioxidant and Anticancer Activity of Methanolic Extract From Stephania Elegans

Objective: The objective of the present study is to assess the anticancer effect of limnic algae extract of KACC 2 (Karunya Algae Culture Collection) and KACC 23 against MG-63 cell line.Methods: Bioactive compounds were extracted from the algae culture by Soxhlet extraction method using methanol as solvent, and the extract was checked for its anticancer activity. Polyphenols were estimated using Folin-Ciocalteu reagent method. The constituents of the extract were analyzed using gas chromatography-mass spectrometry (GC-MS).Results: The results showed that KACC 2 had more phenolic content than KACC 23. The half maximal inhibitory concentration value for KACC 2 and 23 was found as 0.386 μl/ml and 0.285 μl/ml of extracts necessary for the 50% of cell death. GC-MS analysis revealed that the compounds with possible therapeutic effects are quercetin, stigmast-4-en-3-one, epoxygedunin, which has got anticancer activity.Conclusion: These observations point out that the algae extract of KACC 2 and KACC 23 has anticancer property and the phytoconstituents contributed to the anticancer effects.

Section: Determination Of Total Polyphenol Contentmentioning

confidence: 99%

Anticancer Activity of Micro-Algae Extract on Human Cancer Cell Line (Mg-63)

James

Thomas

2019

“…Antioxidant nature of plant-based compounds plays a significant role in controlling many ailments including the dreadful cancer [54][55][56]. Vitamin C, a known antioxidant, exerts its effects both through acting as antioxidant and through non-antioxidant effect.…”

Section: Discussionmentioning

confidence: 99%

Neuroprotective Role of Ascorbic Acid: Antioxidant and Non-Antioxidant Functions

Kumar

Saini

2018

Ascorbic acid (AA) or Vitamin C is an important antioxidant which participates in numerous cellular functions. Although in human plasma its concentration is in micromolars but it reaches millimolar concentrations in most of the human tissues. The high ascorbate cellular concentrations are generated and maintained by a specific sodium-dependent Vitamin C transporter type 2 (SVCT2, member of Slc23 family). Metabolic processes recycle Vitamin C from its oxidized forms (ascorbate) inside the cells. AA concentration is highest in the neurons of the central nervous system (CNS) of mammals, and deletion of its transporter affects mice brain and overall survival. In the CNS, intracellular ascorbate serves several functions including antioxidant protection, peptide amidation, myelin formation, synaptic potentiation, and protection against glutamate toxicity. SVCT2 maintains neuronal ascorbate content in CNS which has relevance for neurodegenerative diseases such as Alzheimer’s, Parkinson’s, and Huntington’s disease. As ascorbate supplements decrease infarct size in ischemia-reperfusion injury and protect neurons from oxidative damage, it is a vital dietary antioxidant. The aim of this review is to assess the role of the SVCT2 in regulating neuronal ascorbate homeostasis in CNS and the extent to which ascorbate affects brain function as an antioxidant.

“…In a similar study through MTT assay, IC 50 of Datura metel leaf and stem extract was evaluated against Vero and MCF7 cell line [19]. In another study, IC 50 of Stephania elegans on MCF7 was reported to be 158.7 ± 0.13 µg/ml [20].…”

Section: Anticancer Assaysmentioning

confidence: 99%

Anticancer Activity of Garcinia Morella Chloroform Fraction and Its Active Compound Garcinol on Neuroblastoma

Choudhury

Kandimalla

Bharali

et al. 2017

Objective: The aim of the present study is to evaluate the anticancer activity of G. morella fruit chloroform fraction and its isolated bioactive molecule garcinol on neuroblastoma cell line (SH-SY5Y).Method: Methanol extraction was performed for the G. morella fruits through cold maceration and further fractionated with chloroform. The presence of Garcinol was confirmed by measuring the melting point. Further, the bioactive chloroform fraction and pure Garcinol was tested for anticancer activity against SH-SY5Y cells through MTT assay. Result:The present study reveals the anticancer ability of bioactive chloroform fraction of G. morella fruit and its active molecule garcinol. Conclusion:G. morella fruit and its bioactive compound Garcinol have significant activity against neuroblastoma. This study opens an avenue to further elucidate the mechanism of action and development of alternative treatment of this dreaded disease.