Antioxidant and antibacterial activity of Trametes polyzona (Pers.) Justo

Adongbede, Erute Magdalene; Jaiswal, Yogini; Davis, Shurrita S.; Randolph, Priscilla; Huo, Lini; Williams, Leonard

doi:10.1007/s10068-019-00642-4

Cited by 6 publications

(2 citation statements)

References 31 publications

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“…Mushrooms of the genus Trametes are polypore and have therapeutic properties for the liver, kidneys, and heart, as well as immune system support [22]. Polypores are global mushrooms that have been extensively studied for their potential to combat multidrugresistant infections [23].…”

Section: Discussionmentioning

confidence: 99%

MOLECULAR IDENTIFICATION AND ANTIBACTERIAL ACTIVITY OF MACROFUNGUS Trametes sanguineus (L.)

Dandapat

2023

Biotechnol. acta

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Aim. The molecular identification of Pycnoporus sanguineus, a previously morphologically mushroom, was done to see the antibacterial activity against pathogenic bacteria Staphylococcus aureus and Salmonella typhi. Methods. A fragment of the D2 region of 28S rDNA was amplified by PCR, sequenced, and BLAST was performed using the consensus sequence. The maximum identity score was used to build a phylogenetic tree. Agar well diffusion was used to study the antibacterial activity. Results. Sequencing of a 700 base pair PCR amplicon was carried and a 616 base pair of D2 region of large subunit gene was generated. The 100 blast hits on the D2 region of LSU gene showed similarity to Trametes sanguineus voucher PRSC95 (GenBank Accession Number: JN164795.1) based on nucleotide homology and phylogenetic analysis. Antibacterial screening revealed that the crude extract had higher activity on Staphylococcus aureus, with a 3mm to 13mm zone of inhibition and a 100µg minimum inhibitory concentration, compared to Salmonella typhi. Salmonella typhi had a 5 mm to 15 mm zone of inhibition and a 200 µg minimum inhibitory concentration. Conclusion. According to the obtained result, the morphologically identified mushroom Pycnoporus sanguies can be referred to as Trametes sanguine, and it can be used for producinig antibacterial agents.

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Section: Discussionmentioning

confidence: 99%

MOLECULAR IDENTIFICATION AND ANTIBACTERIAL ACTIVITY OF MACROFUNGUS Trametes sanguineus (L.)

Dandapat

2023

Biotechnol. acta

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“…This fungus has been reported to secrete various enzymes, such as laccase and manganese peroxidase, which demonstrate the degradation of aromatic compounds in a polluted environment and high tolerance for biodegradation ( 17 , 18 ). In a previous study, the phenolic extract of T. polyzona was found to have antimicrobial abilities against various bacterial strains and antioxidant properties against several radicals ( 19 ). However, to the best of our knowledge, no evaluation of other bioactive compounds from this fungus, such as polysaccharides, in cancer treatment has been performed.…”

Section: Introductionmentioning

confidence: 99%

Antiproliferative activity and apoptosis‑inducing effects of Trametes polyzona polysaccharides against human breast cancer cells

Kurniawan,

Bankeeree,

Yanatatsaneejit

et al. 2023

Biomed Rep

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Fungal polysaccharides have garnered interest due to their biological activities in terms of anticancer properties and antioxidant activity. The present study aimed to evaluate the anticancer properties and antioxidant activity of a newly isolated white-rot fungus, Trametes polyzona CU07 from Thailand. Crude T. polyzona polysaccharides (CTPPs) were extracted from mycelia using hot water. The chemical properties, including total carbohydrates, molecular weight and protein content, and Fourier-transform infrared spectroscopy analysis, were then investigated. The antioxidant activity was determined against the radicals 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). The anticancer properties were evaluated in MCF-7 breast cancer (BC) cells, whereas the 293 cell line was used as a control. The inhibitory effects of CTPPs on viability were determined by MTT assay, followed by BrdU incorporation assay to assess cell proliferation. The induction of apoptosis was determined by flow cytometry. CTPPs were considered polysaccharide-protein conjugates, which had molecular weights in the range of 0.3-22,528 kDa. They contained ~50 and 37% carbohydrate and protein, respectively, with glucose as the main monosaccharide component. Notably, CTPPs had high antioxidant activity against ABTS, and had a significant inhibitory effect on the MCF-7 cell line with a half-maximal inhibitory concentration value of 0.58 mg/ml. However, they exhibited little effect on the 293 cell line. The BrdU incorporation assay demonstrated that CTPPs inhibited proliferation by ~20% compared with that in untreated cells. CTPPs also induced early- and late-stage apoptosis of MCF-7 cells. These results indicated that the CTPPs may exhibit potential antiproliferative and antioxidant activity, and apoptosis-inducing effects against human BC cells.

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