Antioxidant and antibacterial activities of polysaccharides isolated and purified from Diaphragma juglandis fructus

Meng, Qingran; Li, Yinghao; Xiao, Tiancun; Zhang, Lianfu; Xu, Dan

doi:10.1016/j.ijbiomac.2017.07.062

Cited by 69 publications

(32 citation statements)

References 48 publications

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“…This may be due to the fact that the ethanolic extracts contain more biologically active phytochemicals than the chloroform extract. Overall, results revealed that extracts are potential source of bioactive compounds and these findings are in line with previous studies (Bajalan et al, 2017;Fakhfakh et al, 2017;Giacomini et al, 2017;Meng et al, 2017;Nair et al, 2017;Palanisamy et al, 2017;Paliwal et al, 2017;Sagbo et al, 2017).…”

Section: Antibacterial Activities Of Fruit Extractssupporting

confidence: 92%

Comparative study of the antioxidant and antibacterial activities of two guava (Psidium guajava) fruit varieties cultivated in Andasa Horticulture Site, Ethiopia

Int¹

2019

Preprint

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This investigation was carried out to evaluate the levels of phytochemicals and antioxidant potential as well as antibacterial activity of the two varieties of guava (Psidium guajava) fruit collected from Andasa horticulture site, Ethiopia. The antioxidant potential for guava fruit extracts was assessed using different in-vitro antioxidant assays, namely reducing power, Folin-Ciocalteu (F-C) assay and aluminum chloride methods. Overall, the white flesh guava fruit exhibited the highest levels of TPC, TFC and reducing power than the pink flesh guava. The mg/100g of AEAC (FRAP assay), AEAC (PMA), GAE and QE of pink and white flesh guava fruits determined in this study were 125.73, 260.28, 262.78, 377.18, 21.25, 24.61, 7.05 and 22.10, respectively. Antibacterial property of the two guava fruit variety extracts was evaluated against three gram-positive and two gram-negative bacteria using agar well diffusion technique. Ethanol extracts from both pink and white flesh guava exhibited antibacterial activity with zone of inhibition ranging from 29.50 to 30.33 and 24.33 to 29.33 mm respectively. On the other hand, only the chloroform extract from pink flesh guava showed poor antibacterial activity against MRSA and Shigella pathogens among the five tested organisms and the zone of inhibition measured was 11 and 10 mm respectively.

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Section: Antibacterial Activities Of Fruit Extractssupporting

confidence: 92%

Comparative study of the antioxidant and antibacterial activities of two guava (Psidium guajava) fruit varieties cultivated in Andasa Horticulture Site, Ethiopia

Int¹

2019

Preprint

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“…Diaphragma juglandis fructus contains a variety of components including phenolic acids and flavonoid as the above identified compounds. These compounds may have potent anti-inflammatory activities because Diaphragma juglandis fructus has been used in folk medicine to treat kidney deficiency and reproductive diseases since ancient times [ 12 ]. To confirm this hyphotesis, the anti-inflammatory activities of the isolated compounds have been evaluated herein by measuring the effect on NO production.…”

Section: Resultsmentioning

confidence: 99%

“…In view of these important biological activities, a large number of compounds are urgently needed for bioactive research. Polysaccharides purified from Diaphragma juglandis fructus showed strong antibacterial and antioxidant activities [ 12 ]. These promising results led us to continue with further studies in order to get a deeper knowledge on Diaphragma juglandis fructus.…”

Section: Introductionmentioning

confidence: 99%

Chemical Constituents of the Ethyl Acetate Extract from Diaphragma juglandis Fructus and Their Inhibitory Activity on Nitric Oxide Production In Vitro

Wang

Dong

et al. 2017

Molecules

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Diaphragma juglandis fructus contains various bioactive constituents. Fourteen compounds were isolated from Diaphragma juglandis fructus by preparative high performance liquid chromatography (pre-HPLC) and high-speed counter-current chromatography (HSCCC). Their structures were identified by nuclear magnetic resonance (NMR) and electrospray ionization mass spectrometry (ESI-MS). Compounds (+)-dehydrovomifoliol (12), (6R,9R)-9-hydroxymegastigman-4-en-3-one (13) and (6R,9S)-9-hydroxymegastigman-4-en-3-one (14) are found from Juglans regia L. for the first time. Compounds dihydrophaseic acid (2), blumenol B (3) and (4S)-4-hydroxy-1-tetralone (11) are isolated from Diaphragma juglandis fructus for the first time. The anti-inflammatory effects of isolated compounds were evaluated by an in vitro model of lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Compounds gallic acid (1), ethyl gallate (9) and (+)-dehydrovomifoliol (12) exhibited inhibitory activity on the nitric oxide production of RAW 264.7 at a concentration of 25 μM. The result indicated that the combination HSCCC with pre-HPLC is an effective way for compound separation and purification. And Diaphragma juglandis fructus constituents have the potential for the treatment of inflammatory-related diseases.

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“…Additionally, WLP exhibited the highest ABTS scavenging ability of 94.8% at a concentration of 0.8 mg/mL, which was close to the highest ABTS scavenging ability of ascorbic acid (99.42%). Previous studies have reported that the ABTS scavenging activity of gracilaria rubra crude polysaccharides was 55.34% at the concentration of 2.5 mg/mL and a polysaccharide named DJP-2 from diaphragma juglandis fructus demonstrated ABTS radical scavenging function of 78.31% at a concentration of 4 mg/mL (Di et al, 2017;Meng et al, 2017).…”

Section: Abts Free Radical Scavenging Activitymentioning

confidence: 98%

“…ABTS is extensively usedto evaluate the scavenging power of natural products extracted from various plants (Meng et al, 2017). Therefore, ABTS free radical was also applied to assess the antioxidant property of WLP in our examination.…”

Section: Abts Free Radical Scavenging Activitymentioning

confidence: 99%

Optimization for the Extraction of Polysaccharide from Walnut (Juglans regia L.) Leaves: Antioxidant Activities in Vitro

Yang¹,

Xiao²,

Yu³

et al. 2019

American Journal of Biochemistry and Biotechnology

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Cellulase-assisted extraction of Walnut Leaf Polysaccharide (WLP) was studied. Single factor test and response surface design were employed to optimize the technological conditions. The consequences indicated that the highest WLP yield of 5.21% was achieved with raw material ratio of 50 mL/g, cellulase dose of 41488.52 U/g, enzymolysis pH of 5.88, enzymolysis time of 29.74 min and enzymolysis temperature of 52.07°C. Moreover, in vitro antioxidant assays revealed that WLP has significant eliminating capability against 1, 1-diphenyl-2-picrylhydrazyl (DPPH), 2, 2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and hydroxyl free radicals in a dose-dependent manner and exhibited a strong reducing power. These results suggest that WLP could be developed as a promising natural antioxidant agent in the pharmaceutical and functional food industries.

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Antioxidant and antibacterial activities of polysaccharides isolated and purified from Diaphragma juglandis fructus

Cited by 69 publications

References 48 publications

Comparative study of the antioxidant and antibacterial activities of two guava (Psidium guajava) fruit varieties cultivated in Andasa Horticulture Site, Ethiopia

Comparative study of the antioxidant and antibacterial activities of two guava (Psidium guajava) fruit varieties cultivated in Andasa Horticulture Site, Ethiopia

Chemical Constituents of the Ethyl Acetate Extract from Diaphragma juglandis Fructus and Their Inhibitory Activity on Nitric Oxide Production In Vitro

Optimization for the Extraction of Polysaccharide from Walnut (Juglans regia L.) Leaves: Antioxidant Activities in Vitro

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