Antioxidant and Antiangiogenic Properties of Phenolic Extract from Pleurotus tuber-regium

Abstract

Pleurotus tuber-regium (Fries) Singer (PTR), both an edible and a medicinal mushroom also known as tiger milk mushroom, has experienced growing popularity and economic importance due to its flavor, nutritive value, and medicinal effects. In this study, the antioxidant and antiangiogenic activities of a 60% ethanol extract (EE) obtained from the sclerotium of PTR were investigated. Typical phenolic compounds including protocatechuic, chlorogenic, syringic, ferulic, and folic acid were identified and quantified in EE by the HPLC-UV-ESI/MS analyses. EE possessed strong antioxidant activity and could dose-dependently inhibit vascular endothelial growth factor (VEGF)-induced human umbilical vein endothelial cells (HUVEC) migration and tube formation. qPCR results showed that VEGF-induced FGF, ANG-Tie, and MMP gene expression as well as VEGFR were down-regulated at the mRNA level after treated with EE, suggesting that multiple molecular targets related to angiogenesis was involved. Furthermore, EE also inhibited the formation of subintestinal vessel plexus (SIVs) in zebrafish embryos in vivo. All of these suggested that EE of PTR could be the source of potential inhibitors to target angiogenesis.

Comparative study of antioxidant and antibacterial properties of the edible mushrooms Pleurotus levis, P. ostreatus, P. pulmonarius and P. tuber‐regium

“…These results were in agreement with previous correlation reports in P. ostreatus, P. cystidiosus and P. tuberregium (Yang et al, 2002;Lin et al, 2014;Smolskait_ e et al, 2015;Koutrotsios et al, 2017), suggesting a prevalent trend within the genus Pleurotus. This linear correlation showed that increasing scavenging activity corresponded to increasing content of total phenolic compounds.…”

“…Gursoy et al (2009) used methanolic extracts of wild mushrooms, Morchella spp., and also found low antioxidant activity (4.5 mg mL À1 ) against the b-carotene-linoleic acid. A 60% ethanol extract from the sclerotium of P. tuber-regium (Lin et al, 2014) contained high total phenolic content (420 GAE lg mg À1 of extract) and showed DPPH free radical scavenging activity (1 mg mL À1 ) and ABTS radical scavenging activity (5 mg mL À1 ). Pleurotus ostreatus has also been studied using modifications of the techniques, and these data are given as a reference.…”

Reactivity of phenolic compounds towards free radicals under in vitro conditions

“…The redox potentials of hydroxycinnamic acid derivatives are lower than that of oxy-radicals such as the hydroxyl radical E 7 = 1.9 V, superoxide radical anion E 7 = 0.94 V (Wardman 1989) which means that they are excellent scavengers of these oxy radicals. Compounds that possess more than one hydroxyl group in their aromatic ring like propyl gallate, gallic acid, caffeic acid, pyrogallol and protocatechuic acid exhibited stronger inhibitory potency than monohydroxyl substituents like p-coumaric acid and ferulic acid ( Protocatechuic acid and ferulic acid identified as active components in Pleurotus tuber, an edible mushroom has been reported to exhibit antioxidant and antiangiogenic properties (Lin et al 2014). …”

“…The protective effects of diets, rich in vegetables, fruits and cereals against various forms of cancer and cardiovascular diseases (De Ancos et al 2000;Nicodemus et al 2001) have been attributed, in large to the antioxidant nutrients and plant phenolics such as flavonoids, benzoic acid derivatives and phenylpropanoids (Lin et al 2014;Pacifico et al 2014;Hermann 1989). In addition to t2he free radical scavenging capacity (Lucini et al 2015;Heo et al 2014) plant phenolics have multiple biological activities including vasodilatory, anticarcinogenic, anti-inflammatory, antibacterial, immune stimulating, anti-allergic, antiviral and estrogenic effects (Heo et al 2014;Crozier et al 2009;Kumbulainen and Salonen 1999).…”

Inhibitory effect of <i>Labisia pumila</i> leaf extract on angiogenesis via down-regulation of vascular endothelial growth factor

“…Both ME and WF were shown to have comparable total phenolic contents. This suggested that the anti-angiogenic activity displayed by both was largely due to the presence of some bioactive phenolic phytochemical components [28,29] Extracts rich with phenolic compounds had previously been shown to exert remarkable antiangiogenic effects [30].…”