Antioxidant and anti‐tyrosinase activity of a novel stilbene analogue as an anti‐browning agent

Abstract

BACKGROUND: Tyrosinase inhibitors find potential application in food, cosmetic and medicinal products, but most of the identified tyrosinase inhibitors are not suitable for practical use because of safety regulations or other problems. For the purpose of development of novel tyrosinase inhibitors that meet the requirement for practical application, a novel stilbene analogue (SA) was designed.RESULTS: SA was found to possess a potent inhibitory effect against both mono-and diphenolase activities of mushroom tyrosinase, with IC 50 values of 1.56 and 7.15 ∼mol L -1 , respectively. Compared with a natural tyrosinase inhibitorkojic acidthe anti-tyrosinase effect of SA was significantly improved. Analysis of inhibition kinetics indicated that SA was a reversible and competitive-noncompetitive mixed-type inhibitor. SA was also found to possess more potent antioxidant activities (DPPH, superoxide anion radical and hydroxyl radical scavenging ability) than those of kojic acid. Cell viability studies revealed that SA was non-toxic to two cell lines. Furthermore, an anti-browning test demonstrated that SA effectively delayed the blackening of shrimp.CONCLUSION: SA has potential as an anti-browning agent in foods.

Synthesis of 3-hydroxypyridin-4-one derivatives bearing benzyl hydrazide substitutions towards anti-tyrosinase and free radical scavenging activities

“…23 Due to their copper chelation ability, they were used as tyrosinase enzyme inhibitors. [24][25][26] In a recent study to search the possible application of hydroxypyridine (HP) as a tyrosinase inhibitor, some novel hydroxy pyridinone (compound I and II) was reported as tyrosinase inhibitors with IC 50 = 1.95 mM and 2.79 mM against tyrosinase in diphenolase activity, respectively. 27 In 2016, hydroxypyridinone derivatives containing an oxime ether moiety (compound III and IV) were found to be another potent inhibitor with an IC 50 value of 2.04 mM and 1.60 mM against monophenolase activity 28 (Fig.…”