Antioxidant and Anti-inflammatory Effects of Ethanol Extracts from Perilla frutescens

Abstract

We investigated the effects of an ethanol extract from Perilla frutescens sprouts (PFSE) as an antioxidant, and its effects on edema and inflammation in RAW 264.7 cells and HMC-1 cells. The antioxidant activities (DPPH and ABTS radical scavenging) of PFSE were similar to those of butylated hydroxytoluene (BHT) and (±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox). We also investigated the anti-inflammatory effects of PFSE on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells and HMC-1 cells stimulated with phorbol 12-myristate 13-acetate (PMA) with the calcium ionophore A23187. TNF-α and IL-1β production, which had been increased by treatment with LPS or PMA plus A23187, were significantly inhibited by PFSE in a dose-dependent manner. Furthermore, PFSE significantly reduced the xylene-induced ear edema and the carrageenan-induced paw edema of ICR mice in a dosedependent manner. The effects of PFSE (200 mg/kg) in reducing ear and paw edema were similar to those of aspirin (50 mg/kg). These results suggest that PFSE can be potentially used as a medicine for treating oxidative stress, an edematous and inflammatory disease.

<i>In vitro</i> antioxidative activity of moss extract, and effect of moss on serum lipid level of mice fed with high-fat diet

“…While direct comparisons of these results to other mosses are not currently possible, a variety of studies evaluating the effects of various plant extracts [18][19][20][21][22] are available. The polyphenol contents of teas prepared from domestic esculent plants, such as black tea, ginseng tea, and green tea have been reported to be 101.51, 28.30, and 94.90 μg/mg, respectively [19].…”

“…Ethanol extracts of five edible spring flowers in Korea such as Forsythia koreana Nakai, Rhododendron mucronulatum Turcz., Prunus avium, Magnolia lilyflora, and Magnolia denudate have been reported high antioxidative activity, which are 12.8 to 46.1 mM Trolox/mg ABTS activity, 1.2 to 43.3 mM Trolox/mg DPPH activity, and 4.1 to 35.4 mM Trolox/mg FRAP activity, respectively [20]. Similarly, an extract derived from Perilla frutescens sprouts was shown to exhibit potent anti-oxidative properties, including the ability to effectively scavenge DPPH and ABTS radicals, anti-inflammatory activity, and antiemetic effects [22].…”

Germination and Growth Characteristics of &lt;italic&gt;Perilla frutescens&lt;/italic&gt; and Nutrients by the Mixed Seeding Rate of &lt;italic&gt;Medicago sativa&lt;/italic&gt; in Indoor Urban Agriculture

“…It has a thin cell wall and easily discharges the nutrients it contains and is thus quickly absorbed by the human body. It is recognized as a health functional food since it is also related to inhibiting cancer cell proliferation, lowering blood pressure, and dissolving blood clots (Jeong et al, 2014), which is why it was selected as the major crop.…”

Evaluating the Impact of Thermal Processing on the Anti-Inflammatory Activity of Non-Centrifugal Cane Sugar: Implications on Cytokine Secretion and TLR4 Signaling

“…Accordingly, there is an increasing interest in the discovery of anti-inflammatory agents with the therapeutic potential of neutralizing excessive and persistent inflammatory reactions ( Jeong et al, 2014 ). In this regard, a variety of steroidal and non-steroidal drugs with potent anti-inflammatory activities have been developed, but their reported side effects during prolonged exposure periods have considerably limited their clinical administration ( del Grossi Moura et al, 2018 ).…”