Antioxidant and anti-inflammatory effects of N-acetylcysteine against malathion-induced liver damages and immunotoxicity in rats

Lasram, Mohamed Montassar; Lamine, Aicha Jrad; Dhouib, Ines Bini; Bouzid, K.; Annabi, Alya; Belhadjhmida, Nadia; Ahmed, Malika Ben; Fazâa, Saloua El; Abdelmoula, J.; Gharbi, N.

doi:10.1016/j.lfs.2014.04.033

Cited by 76 publications

(50 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Extensive data suggest that free radical formation and oxidative stress can be a major contributor to the toxicity of pesticides (108)(109)(110)(111)(112)(113). OPs are oxidants and impair enzymatic antioxidant defences, including superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione S-transferase (GST) (23,34,44,(114)(115)(116)(117)(118)(119)(120)(121)(122)(123)(124)(125)(126)(127)(128)(129). Malathion, methyl parathion, and parathion in HepG2 cell lines, as well as chlorpyrifos, methidathion, chlorfenvinphos, and dimethoate in rat liver and diazinon in fish most probably disrupt membrane lipids through oxidative stress (23,35,61,116,118,(130)(131)(132)(133)(134)(135).…”

Section: Possible Effects Of Ops On Hepatic Oxidative Stress Pathwaysmentioning

confidence: 99%

Adverse effects of organophosphorus pesticides on the liver: a brief summary of four decades of research

Karami‐Mohajeri

Ahmadipour

Rahimi

et al. 2017

Archives of Industrial Hygiene and Toxicology

View full text Add to dashboard Cite

Organophosphorus pesticides (OPs) are widely used volatile pesticides that have harmful effects on the liver in acute and chronic exposures. This review article summarises and discusses a wide collection of studies published over the last 40 years reporting on the effects of OPs on the liver, in an attempt to propose general mechanisms of OP hepatotoxicity and possible treatment. Several key biological processes have been reported as involved in OP-induced hepatotoxicity such as disturbances in the antioxidant defence system, oxidative stress, apoptosis, and mitochondrial and microsomal metabolism. Most studies show that antioxidants can attenuate oxidative stress and the consequent changes in liver function. However, few studies have examined the relationship between OP structures and the severity and mechanism of their action. We hope that future in vitro, in vivo, and clinical trials will answer the remaining questions about the mechanisms of OP hepatotoxicity and its management.

show abstract

Section: Possible Effects Of Ops On Hepatic Oxidative Stress Pathwaysmentioning

confidence: 99%

Adverse effects of organophosphorus pesticides on the liver: a brief summary of four decades of research

Karami‐Mohajeri

Ahmadipour

Rahimi

et al. 2017

Archives of Industrial Hygiene and Toxicology

View full text Add to dashboard Cite

show abstract

“…NAC administration to older females, neutrophil function (adherence, chemotaxis, phagocytosis, and oxidative burst), and oxidative stress were normalized and approached values of younger females further supporting a redox-dependent regulation of neutrophil function [54]. NAC infusion to rats with sepsis resulted in reduced oxidative stress, neutrophil density, and IL-1b [30, 70, 74]. Similarly, neutrophil function was inhibited by a 3% NAC solution in mares and mitigated the ROS-mediated injury to the endometrial epithelium and sperm by attenuating neutrophils' oxidative burst [69].…”

Section: Discussionmentioning

confidence: 97%

“…A decline in respiratory burst has also been seen in healthy volunteers following a 14 d NAC ingestion [49]. NAC-induced attenuation of neutrophils' oxidative burst has been consistently seen in animal tissues as well [69] and it may be attributed to a reduction of myeloperoxidase activity (MPO) [70] and/or cellular ATP [49]. A reduction of MPO could be explained by a direct scavenging action by NAC on ROS produced by MPO, or by noncompetitive mechanism by binding to MPO or even by competitive inhibition at or near the active site of the enzyme by GSH [71].…”

Section: Discussionmentioning

confidence: 99%

Evidence of a Redox‐Dependent Regulation of Immune Responses to Exercise‐Induced Inflammation

Sakelliou

Fatouros

Athanailidis

et al. 2016

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

We used thiol-based antioxidant supplementation (n-acetylcysteine, NAC) to determine whether immune mobilisation following skeletal muscle microtrauma induced by exercise is redox-sensitive in healthy humans. According to a two-trial, double-blind, crossover, repeated measures design, 10 young men received either placebo or NAC (20 mg/kg/day) immediately after a muscle-damaging exercise protocol (300 eccentric contractions) and for eight consecutive days. Blood sampling and performance assessments were performed before exercise, after exercise, and daily throughout recovery. NAC reduced the decline of reduced glutathione in erythrocytes and the increase of plasma protein carbonyls, serum TAC and erythrocyte oxidized glutathione, and TBARS and catalase activity during recovery thereby altering postexercise redox status. The rise of muscle damage and inflammatory markers (muscle strength, creatine kinase activity, CRP, proinflammatory cytokines, and adhesion molecules) was less pronounced in NAC during the first phase of recovery. The rise of leukocyte and neutrophil count was decreased by NAC after exercise. Results on immune cell subpopulations obtained by flow cytometry indicated that NAC ingestion reduced the exercise-induced rise of total macrophages, HLA+ macrophages, and 11B+ macrophages and abolished the exercise-induced upregulation of B lymphocytes. Natural killer cells declined only in PLA immediately after exercise. These results indicate that thiol-based antioxidant supplementation blunts immune cell mobilisation in response to exercise-induced inflammation suggesting that leukocyte mobilization may be under redox-dependent regulation.

show abstract

“…The apoptotic index used in this study followed the nuclei and cytoplasm morphological characteristics of hepatocyte apoptosis [14][15][16] and results showed significant changes during follow-up. In view of the effects of N-acetylcysteine on the apoptotic index of liver subjected to hypothermic preservation hepatocytes, the future prospect of this study is to confirm the apoptosis by immunohistochemical marking, for example, anticaspase 3 cleaved antibody or TUNEL.…”

Section: Discussionmentioning

confidence: 99%

The effect of n-acetylcysteine on hepatic histomorphology during hypothermic preservation

et al. 2014

View full text Add to dashboard Cite

. Tutor. Responsible for conception, design, intellectual and scientific content of the study. Final approval of the version to be published. ABSTRACT PURPOSE:To evaluate the NAC effects on liver hypothermic preservation at different time intervals. METHODS:For this, we used livers of male Wistar rats weighing between 250 and 300g, undergoing preservation in Ringer solution at 4°C for up to 24 hours. Tissue samples were obtained at four moments of preservation for histological examination by hematoxylin and eosin staining: T0 = beginning of preservation, T12 = 12 hours, T18 = 18 hours and T24 = 24 hours. Will be analyzed vacuolation, hepatic apoptosis by optical microscopy and parenchymal. RESULTS:The results showed a progressive increase in hepatic injury in both groups and showed that NAC was effective at T0. The parenchyma preservation was better in the NAC group and no difference when vacuolization of the cells. CONCLUSION:Hypothermic preservation, over time, causes changes in the hepatic parenchyma with increased apoptosis, loss of architecture, vacuolization, culminating in severe injury. The administration of N-acetylcysteine protects against preservation liver injury.

show abstract

Antioxidant and anti-inflammatory effects of N-acetylcysteine against malathion-induced liver damages and immunotoxicity in rats

Cited by 76 publications

References 61 publications

Adverse effects of organophosphorus pesticides on the liver: a brief summary of four decades of research

Adverse effects of organophosphorus pesticides on the liver: a brief summary of four decades of research

Evidence of a Redox‐Dependent Regulation of Immune Responses to Exercise‐Induced Inflammation

The effect of n-acetylcysteine on hepatic histomorphology during hypothermic preservation

Contact Info

Product

Resources

About