Antioxidant and Anti-Inflammatory Activities of Pomegranate (Punica granatum) onEimeria papillata-Induced Infection in Mice

Abstract

Coccidiosis is the most prevalent disease causing widespread economic loss, especially in poultry farms. Here, we investigated the effects of pomegranate peel extract (PPE) on the outcome of coccidiosis caused by Eimeria papillata in mice. The data showed that mice infected with E. papillata and treated with PPE revealed a significant decrease in the output of oocysts in their faeces by day 5 p.i. Infection also induced inflammation and injury of the jejunum. This was evidenced (i) as increases in reactive oxygen species, (ii), as increased neutrophils and decreased lymphocytes in blood (ii) as increased mRNA levels of inducible nitric oxide synthase (iNOS), Bcl-2 gene, and of the cytokines interferon gamma (IFN-γ), tumour necrosis factor-α (TNF-α), and interleukin-1β (IL-1β), and (iv) as downregulation of mucin gene MUC2 mRNA. All these infection-induced parameters were significantly altered during PPE treatment. In particular, PPE counteracted the E. papillata-induced loss of the total antioxidant capacity. Our data indicated that PPE treatment significantly attenuated inflammation and injury of the jejunum induced by E. papillata infections.

Antiinflammatory and anti‐cancer activities of pomegranate and its constituent, ellagic acid: Evidence from cellular, animal, and clinical studies

“…Amer et al evaluated the effects of PPE (300 mg/kg) on Eimeria papillata‐induced Coccidiosis in mice. They showed that PPE makes a significant decrement in the levels of ROS generation, iNOS, Bcl‐2, IFN‐γ, TNF‐α, and IL‐1β, while propagates mucin gene MUC2 mRNA level (Amer et al, ).…”

“…Amer et al evaluated the effects of PPE (300 mg/kg) onEimeria papillata-induced Coccidiosis in mice. They showed that PPE makes a significant decrement in the levels of ROS generation, iNOS, Bcl-2, IFN-γ, TNF-α, and IL-1β, while propagates mucin gene MUC2 mRNA level(Amer et al, 2015).Pancreatitis. Minaiyan and coworkers investigated the effects of pomegranate freeze-dried powder (PFDP), hydro-alcoholic pomegranate seeds extract (PSE, 250 and 500 mg/kg, i.p.…”

Simultaneous determination of polyphenols and triterpenes in pomegranate peel based on high‐performance liquid chromatography fingerprint by solvent extraction and ratio blending method in tandem with wavelength switching

“…Evidence shows that pomegranate peel has a higher content of polyphenols compared with seeds, pulp or juice samples and its antioxidant activity is related to total phenolics (Derakhshan et al, ; Gómez‐Caravaca et al, ; Russo et al, ). Modern studies have shown that pomegranate peel exhibits many pharmacological effects, including antioxidant, antibacterial, anti‐inflammatory, promoting wound healing, immunomodulatory, antidiabetic and anticancer activities (Amer, Dkhil, Hikal, & Al‐Quraishy, ; Emam‐Djomeh, Moghaddam, & Yasini Ardakani, ; Labsi, Khelifi, Mezioug, Soufli, & Touil‐Boukoffa, ; Pagliarulo et al, ; Usha et al, ). Besides, studies have shown that the main components of polyphenols and triterpenes in pomegranate peel exhibit anticancer activities (Jaman & Sayeed, ; Lu, Li, Li, & Aisa, ).…”

Antidiarrheal effect of bioactivity‐guided fractions and bioactive components of pomegranate (Punica granatum L.) peels

“…The biological activities of pomegranate peel extracts have been extensively investigated, including normalization of gastrointestinal movements, inhibition of bacterial growth, prevention of obesity, treatment of cardiovascular disease, and acceleration of wound healing . It also possesses other pharmacological properties, such as strong antioxidant, antimicrobial, anticoccidial, anthelmintic, antidiarrheal, antimalarial, and anti‐inflammatory activities . Other studies reported that pomegranate extracts also have immunomodulatory and antidiabetic activities .…”

“…12 It also possesses other pharmacological properties, such as strong antioxidant, 13 antimicrobial, 14 anticoccidial, anthelmintic, 15 antidiarrheal, 16 antimalarial, and anti-inflammatory activities. 17 Other studies reported that pomegranate extracts also have immunomodulatory and antidiabetic activities. 18 Recent studies have shown that pomegranate polyphenols may be anticancer agents.…”