Antioxidant and Anti-inflammatory Activities of Broccoli Florets in LPS-stimulated RAW 264.7 Cells

Hwang, Joon-Ho; Lim, Sang-Bin

doi:10.3746/pnf.2014.19.2.089

Cited by 40 publications

(31 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…8 , the hydroxyl radical scavenging activity of GANPs was 2 times higher than that of GA at concentrations ranging between 31.5 and 500 µg/mL with IC 50 values of 56.03 µg/mL and 105.53 µg/mL, respectively ( P < 0.05). This result also confirmed that gallic acid maintained its antioxidant properties that were consistent with other reports 32 . Gallic acid was reported to have cyclooxygenase 2 (COX-2) and nitric oxide synthase (iNOS) inhibitory properties in LPS-induced RAW 264.7 cells modulating the pro-inflammatory cytokines such as IL-17, IFN-γ, and TNF-α 33 .…”

Section: Discussionsupporting

confidence: 93%

Preparation, characterization and therapeutic properties of gum arabic-stabilized gallic acid nanoparticles

Hassani

Azarian

Ibrahim

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Gallic acid (GA) is a natural phenolic compound with therapeutic effects that are often challenged by its rapid metabolism and clearance. Therefore, GA was encapsulated using gum arabic into nanoparticles to increase its bioavailability. The formulated nanoparticles (GANPs) were characterized for physicochemical properties and size and were then evaluated for antioxidant and antihypertensive effects using various established in vitro assays, including 1,1-diphenyl-2-picrylhydrazyl (DPPH), nitric oxide scavenging (NO), β-carotene bleaching and angiotensin-converting enzyme (ACE) inhibitory assays. The GANPs were further evaluated for the in vitro cytotoxicity, cell uptake and cell migration in four types of human cancer cell lines including (MCF-7, MDA-MB231) breast adenocarcinoma, HepG2 hepatocellular cancer, HT-29 colorectal adenocarcinoma, and MCF-10A breast epithelial cell lines. The GANPs demonstrated potent antioxidant effects and have shown promising anti-cancer properties in a dose-dependent manner with a predilection toward HepG2 and MCF7 cancer cells. The uptake of GANPs was successful in the majority of cancer cells with a propensity to accumulate in the nuclear region of the cells. The HepG2 and MCF7 cancer cells also had a significantly higher percentage of apoptosis and were more sensitive to gallic acid nanoparticle treatment in the cell migration assay. This study is the first to confirm the synergistic effects of gum arabic in the encapsulation of gallic acid by increasing the selectivity towards cancer cells and enhancing the antioxidant properties. The formulated nanoparticles also had remarkably low toxicity in normal cells. Based on these findings, GANPs may have promising therapeutic applications towards the development of more effective treatments with a probable targeting precision in cancer cells.

show abstract

Section: Discussionsupporting

confidence: 93%

Preparation, characterization and therapeutic properties of gum arabic-stabilized gallic acid nanoparticles

Hassani

Azarian

Ibrahim

et al. 2020

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Treating macrophages with OBE before the stimulation with LPS partly counteracted such responses by about 30%. These results are similar to findings from recent studies demonstrating anti-inflammatory properties of polyphenols and other plant bioactives in cell cultures [52; 53] and farm animals [54]. Furthermore, our in vivo and in vitro results indicate that inhibition of IL1B production is a key component of the OBE anti-inflammatory action.…”

Section: Discussionsupporting

confidence: 92%

Olive oil bioactives protect pigs against experimentally-induced chronic inflammation independently of alterations in gut microbiota

Liehr

Mereu²,

Pastor³

et al. 2017

PLoS ONE

View full text Add to dashboard Cite

Subclinical chronic inflammation (SCI) is associated with impaired animal growth. Previous work has demonstrated that olive-derived plant bioactives exhibit anti-inflammatory properties that could possibly counteract the growth-depressing effects of SCI. To test this hypothesis and define the underlying mechanism, we conducted a 30-day study in which piglets fed an olive-oil bioactive extract (OBE) and their control counterparts (C+) were injected repeatedly during the last 10 days of the study with increasing doses of Escherichia coli lipopolysaccharides (LPS) to induce SCI. A third group of piglets remained untreated throughout the study and served as a negative control (C-). In C+ pigs, SCI increased the circulating concentration of interleukin 1 beta (p < 0.001) and decreased feed ingestion (p < 0.05) and weight gain (p < 0.05). These responses were not observed in OBE animals. Although intestinal inflammation and colonic microbial ecology was not altered by treatments, OBE enhanced ileal mRNA abundance of tight and adherens junctional proteins (p < 0.05) and plasma recovery of mannitol (p < 0.05) compared with C+ and C-. In line with these findings, OBE improved transepithelial electrical resistance (p < 0.01) in TNF-α-challenged Caco-2/TC-7 cells, and repressed the production of inflammatory cytokines (p < 0.05) in LPS-stimulated macrophages. In summary, this work demonstrates that OBE attenuates the suppressing effect of SCI on animal growth through a mechanism that appears to involve improvements in intestinal integrity unrelated to alterations in gut microbial ecology and function.

show abstract

“…The TPC of each extract was determined using Folin‐Ciocalteu's method (Hwang and Lim ). A total of 200 μL of the extract, 800 μL distilled water, and 100 μL 2 M Folin‐Ciocalteu phenolic reagent were combined in a glass vial and mixed by vortexing.…”

Section: Methodsmentioning

confidence: 99%

Formulation Optimization of Antioxidant-Rich Juice Powders Based on Experimental Mixture Design

Kim

Lim

2016

Journal of Food Processing and Preservation

Self Cite

View full text Add to dashboard Cite

This study optimized the mixing ratio of broccoli (BroMP), cabbage (CabMP), and carrot-mixed powders (CarMP) for the development of juice powders containing high total phenolic content (TPC), high antioxidant activities, and preferable sensory properties using a mixture design. TPC and antioxidant activities of juice powders were increased with a higher proportion of BroMP and lower proportions of CabMP and CarMP. However, the overall acceptance was increased with a higher CarMP proportion and lower BroMP and CabMP proportions. The optimal mixing ratio was 67.4% BroMP, 16.7% CabMP, and 15.9% CarMP. At this ratio, the predicted response values of TPC, ABTS radical scavenging activity (IC 50 ), FRAP activity, and overall acceptance were 9.51 mg GAE/g, 5.35 lg/mL, 81.3 mM FSE/g, and 4.62, respectively. The optimized mixed juice powder showed high sums of individual phenolic compounds, with chlorogenic acid (121.16 mg/100 g) and quercetin (71.74 mg/100 g) as the major phenolics. PRACTICAL APPLICATIONSVegetable juice powder containing BroMP, CabMP, and CarMP can be utilized as a functional drink having high antioxidant activities and preferable sensory properties. The optimized juice powder contained higher contents of individual phenolic compounds than those of the broccoli or cabbage powder. Thus, mixed vegetable powders can provide large amounts of diverse phenolic compounds from different vegetable sources. The mixture design approach was found to be a suitable method for optimizing a healthy and delicious juice formulation, and can be effectively applied in other food mixture systems.

show abstract

Antioxidant and Anti-inflammatory Activities of Broccoli Florets in LPS-stimulated RAW 264.7 Cells

Cited by 40 publications

References 30 publications

Preparation, characterization and therapeutic properties of gum arabic-stabilized gallic acid nanoparticles

Preparation, characterization and therapeutic properties of gum arabic-stabilized gallic acid nanoparticles

Olive oil bioactives protect pigs against experimentally-induced chronic inflammation independently of alterations in gut microbiota

Formulation Optimization of Antioxidant-Rich Juice Powders Based on Experimental Mixture Design

Contact Info

Product

Resources

About