Antioxidant and anti-inflammatory activites of Clerodendrum leaf extracts collected in Thailand

Phosrithong, Narumol; Nuchtavorn, Nantana

doi:10.1016/j.eujim.2015.10.002

Cited by 35 publications

(23 citation statements)

References 17 publications

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“…Each dried root extract (1 g each) of G. collinum was reconstituted in 20 mL of 70% methanol and this extract was used for the determination of total polyphenolic compounds and total flavonoids contents. Total polyphenolic compounds were determined by Folin-Ciocalteau [ 25 , 26 ] method using gallic acid as the reference standard in the concentration range of 0.02 mg/mL to 0.2 mg/mL in water. Amount of total flavonoids were estimated using the procedure adopted by Numonov et al [ 27 ].…”

Section: Methodsmentioning

confidence: 99%

Evaluation of the Antidiabetic Activity and Chemical Composition of Geranium collinum Root Extracts—Computational and Experimental Investigations

et al. 2017

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The root of Geranium collinum Steph is known in Tajik traditional medicine for its hepatoprotective, antioxidant, and anti-inflammatory therapeutic effects. The present study was conducted to evaluate of potential antidiabetic, antioxidant activities, total polyphenolic and flavonoid content from the different extracts (aqueous, aqueous-ethanolic) and individual compounds isolated of the root parts of G. collinum. The 50% aqueous-ethanolic extract possesses potent antidiabetic activity, with IC50 values of 0.10 μg/mL and 0.09 μg/mL for the enzymes protein-tyrosine phosphatase (1B PTP-1B) and α-glucosidase, respectively. Phytochemical investigations of the 50% aqueous-ethanolic extract of G. collinum, led to the isolation of ten pure compounds identified as 3,3′,4,4′-tetra-O-methylellagic acid (1), 3,3′-di-O-methylellagic acid (2), quercetin (3), caffeic acid (4), (+)-catechin (5), (–)-epicatechin (6), (–)-epigallocatechin (7), gallic acid (8), β-sitosterol-3-O-β-d-glucopyranoside (9), and corilagin (10). Their structures were determined based on 1D and 2D NMR and mass spectrometric analyses. Three isolated compounds exhibited strong inhibitory activity against PTP-1B, with IC50 values below 0.9 μg/mL, more effective than the positive control (1.46 μg/mL). Molecular docking analysis suggests polyphenolic compounds such as corilagin, catechin and caffeic acid inhibit PTP-1B and β-sitosterol-3-O-β-d-gluco-pyranoside inhibits α-glucosidase. The experimental results suggest that the biological activity of G. collinum is related to its polyphenol contents. The results are also in agreement with computational investigations. Furthermore, the potent antidiabetic activity of the 50% aqueous-ethanolic extract from G. collinum shows promise for its future application in medicine. To the best of our knowledge, we hereby report, for the first time, the antidiabetic activity of G. collinum.

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Section: Methodsmentioning

confidence: 99%

Evaluation of the Antidiabetic Activity and Chemical Composition of Geranium collinum Root Extracts—Computational and Experimental Investigations

et al. 2017

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“…DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity of extract were measured according to Zou et al [45]. The anti-inflammatory activity was evaluated as described by Phosrithong & Nuchtavorn with slight modifications described by Yıldırım et al [46,47].…”

Section: In Vitro Antioxidant and Anti-inflammatory Activitymentioning

confidence: 99%

The effects of Urtica dioica L. ethanolic extract against urinary calculi in rats

KELEŞ¹,

Şen²,

Ertaş³

et al. 2020

jrp

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Nephrolithiasis is common urological problem and stone formation has multiple underlying pathogenetic factors. We investigated the possible preventive and therapeutic effects of Urtica dioica ethanol extract (UD) on ethylene glycol-induced nephrolithiasis model in rats. Sprague-Dawley rats were divided into four groups (n = 10). The control group was given normal drinking water for 8 weeks and was administered vehicle by gastric gavage. Stone formation was induced by adding 0.75% ethylene glycol (EG) to their drinking water. UD (700 mg/kg) was given orally for 8 weeks to the preventive group and for last 4 weeks to the treatment group, respectively. At the end of the experiment, urine, blood samples and kidney tissues were obtained. In 24-hour urine samples, calcium and citrate levels were decreased and oxalate levels were increased in EG group whereas UD treatment groups reversed these parameters back to control levels. In addition, serum levels of creatinine and urea were increased in EG group, while UD significantly reduced these parameters. Malondialdehyde, 8-hydroxydeoxyguanosine and tumor necrosis alpha levels, and caspase-3 and N-acetyl-β-glucosaminidase activities were elevated in EG group and showed a decrease in UD treated groups. Glutathione level was decreased in EG group, whereas it was increased in UD preventive group. Histological examination showed an improvement in UD treated groups. Our results suggest that UD is effective both in prevention and treatment for kidney stones. The mechanism underlying this effect may be the antioxidant effect of UD and the effect on the concentration of stone-forming components in the urine.

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“…The anti-inflammatory activity was evaluated as described by Phosrithong and Nuchtavorn with slight modifications described by Yıldırım et al (Phosrithong & Nuchtavorn, 2016;Yıldırım, Şen, Doğan, & Bitiş, 2019). Ten microlitres at different concentrations of the extract (5000-9.77 µg/ml) or standard indomethacine (250-0.49 µg/ml) was added to a 20-μl ethanol, 20-μl pure water, and 25-μl sodium borate buffer solution (0.1 M, pH 9) followed by the addition of 25 μl of a type V soybean lipoxygenase solution in a buffer (pH 9, 20,000 U/ml).…”

Section: In Vitro Antioxidant and Antiinflammatory Activitymentioning

confidence: 99%

Myrtus communisleaf extract protects against cerulein‐induced acute pancreatitis in rats

et al. 2019

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In this study, the aim was to examine the potential protective effects of Myrtus communis subsp. communis leaf ethanol extract (MC) treatment against acute pancreatitis (AP) in rats. Thirty‐two rats were grouped as the saline‐pretreated control (C), MC‐pretreated control (MC), saline‐pretreated AP (AP), and MC‐pretreated AP (MC + AP) groups. To induce AP, cerulein was administered (50 µg/kg) two times. The rats were given MC for 14 days before cerulein injection. Six hours after the final cerulein injection, the rats were sacrificed. Pancreatic damage was associated with an increase in the serum activity of lipase and amylase, the pancreatic activity of myeloperoxidase, and the pancreatic level of malondialdehyde, interleukin‐1β, and interleukin‐6. AP also led to a decrease in the pancreatic level of anti‐inflammatory interleukin‐10 and glutathione. Pretreatment with MC before the induction of AP significantly reduced the pancreatic damage observed during the histological examination as well as reversed the biochemical changes evoked by AP. Practical applications Acute pancreatitis is characterized by high mortality (average about 5%; severe cases may reach about 30%). The current treatment for acute pancreatitis is mainly symptomatic. The introduction of herbal drugs may lead to the development of a new strategy in the treatment of this disease. This study revealed that MC reduced pancreatic injury by decreasing pro‐inflammatory cytokines, increasing antioxidant capacity and anti‐inflammatory cytokine, IL‐10. To the authors’ knowledge, this research is the first report showing that MC inhibits the development of AP. This observation suggests that MC may be useful in the prevention and the treatment of AP in clinical settings.

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Antioxidant and anti-inflammatory activites of Clerodendrum leaf extracts collected in Thailand

Cited by 35 publications

References 17 publications

Evaluation of the Antidiabetic Activity and Chemical Composition of Geranium collinum Root Extracts—Computational and Experimental Investigations

Evaluation of the Antidiabetic Activity and Chemical Composition of Geranium collinum Root Extracts—Computational and Experimental Investigations

The effects of Urtica dioica L. ethanolic extract against urinary calculi in rats

Myrtus communisleaf extract protects against cerulein‐induced acute pancreatitis in rats

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